Difference between revisions of "Team:ZJU-China/Human Practices"

 
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.Retract{
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                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Overview<b class="caret"></b></a>
 
                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Overview<b class="caret"></b></a>
 
                           <ul class="dropdown-menu ">
 
                           <ul class="dropdown-menu ">
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Overview">Project Description</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Overview">Description</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Demonstrate">Demonstrate</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Applied_Design">Applied Design</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Achievements">Achievements</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Achievements">Achievements</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Improve">Improve Parts</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">InterLab</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">InterLab</a></li>
                              <li><a href="https://2017.igem.org/Team:ZJU-China/ImproveParts">Improve Parts</a></li>
+
 
 
                           </ul>
 
                           </ul>
 
                       </li>
 
                       </li>
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                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a>
 
                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a>
 
                           <ul class="dropdown-menu ">
 
                           <ul class="dropdown-menu ">
                              <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Project">Project Home</a></li>-->
 
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/st">Signal Transduction</a></li>
+
                               <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/st">Chemical Signal Transduction</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/ms">Mat Synthesis</a></li>
+
                               <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/mt">Medium Wave Transduction</a></li>
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Project/conclusion">Conclusion</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/Downstream">Downstream</a></li>
                               <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Project/improvement">Improvement</a></li>-->
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Project/conclusion">Conclusions</a></li>
                               <!--<li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">Interlab</a></li>-->
+
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Protocols">Protocols</a></li>
 
                           </ul>
 
                           </ul>
 
                       </li>
 
                       </li>
  
 
                       <li class="m_nav_item dropdown" >
 
                       <li class="m_nav_item dropdown" >
                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Modelling<b class="caret"></b></a>
+
                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Model<b class="caret"></b></a>
 
                           <ul class="dropdown-menu ">
 
                           <ul class="dropdown-menu ">
 
                               <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>-->
 
                               <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>-->
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Model">Coculture</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Model">VOC analysis</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Model/VOC">VOC analysis</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Model/Coculture">Coculture</a></li>
  
 
                           </ul>
 
                           </ul>
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                           <ul class="dropdown-menu ">
 
                           <ul class="dropdown-menu ">
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Parts">All Parts</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Parts">All Parts</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Parts/Basic">Basic Parts</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Basic Parts</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Parts/Composite">Composite Parts</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Composite Parts</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Parts/Collection">Parts Collection</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Part Collection</a></li>
 +
 
 +
 
 +
 
 +
 
 
                           </ul>
 
                           </ul>
 
                       </li>
 
                       </li>
  
                       <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware">Hardware</a></li>
+
                       <li class="m_nav_item dropdown" >
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Hardware<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware">Overview</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Device">Device</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Improvements">Improvements</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/MediumWave">Medium Wave</a></li>
 +
                          </ul>
 +
                      </li>
  
 
                       <li class="m_nav_item dropdown" >
 
                       <li class="m_nav_item dropdown" >
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                           </ul>
 
                           </ul>
 
                       </li>
 
                       </li>
 
                      <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Collaborations">Collaborations</a></li>
 
  
 
                       <li class="m_nav_item dropdown">
 
                       <li class="m_nav_item dropdown">
 
                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">HP<b class="caret"></b></a>
 
                           <a href="#" class="dropdown-toggle link" data-toggle="dropdown">HP<b class="caret"></b></a>
 
                           <ul class="dropdown-menu ">
 
                           <ul class="dropdown-menu ">
 +
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Human_Practices">Summary</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/Human_Practices">Summary</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li>
 
                               <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li>
                               <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold</a></li>
+
                               <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold Integrated</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Engagement">Engagement</a></li>
 
                           </ul>
 
                           </ul>
 
                       </li>
 
                       </li>
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                           </ul>
 
                           </ul>
 
                       </li>
 
                       </li>
 
 
                   </ul>
 
                   </ul>
 
               </div>
 
               </div>
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<div style="margin:0 auto;z-index:50;">
 
<div style="margin:0 auto;z-index:50;">
   <img src="https://static.igem.org/mediawiki/2017/b/be/ZJUChina_interlab_banner.jpg" width="100%">
+
   <img src="https://static.igem.org/mediawiki/2017/1/1b/ZJU_China_HPPublic_248.jpg" width="100%">
 
   <!-- <img src="images/HP_temp/banner.jpg" width="100%"> -->
 
   <!-- <img src="images/HP_temp/banner.jpg" width="100%"> -->
 
</div>
 
</div>
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<div class="timelineLight tl">
 
<div class="timelineLight tl">
 
     <div class="item" data-id="23/05/2017" data-description="Software">
 
     <div class="item" data-id="23/05/2017" data-description="Software">
       <img class="con_borderImage" src="images/hp/timeline/1.jpg" alt="" />
+
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/3/3c/ZJU_China_HP_wx.png" alt="" />
 
       <h2 class="timelineHead">MAY, 23</h2>
 
       <h2 class="timelineHead">MAY, 23</h2>
 
       <span>When your team is on the stage of brainstorming, are you bothered with looking for the former iGEM teams' information? Take out your smart phone and try our Mini Program.
 
       <span>When your team is on the stage of brainstorming, are you bothered with looking for the former iGEM teams' information? Take out your smart phone and try our Mini Program.
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       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/3/33/ZJU_China_HP_high.jpg" alt="" />
 
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/3/33/ZJU_China_HP_high.jpg" alt="" />
 
       <h2 class="timelineHead">MAY, 28</h2>
 
       <h2 class="timelineHead">MAY, 28</h2>
       <span>"We just built up a iGEM high school team, how shall we start?"
+
       <span>"We just built up a iGEM high school team, how shall we start?"<br>
 +
"Don't worry! We can share our experience with you."
 +
 
  
 
</span>
 
</span>
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     <div class="item" data-id="04/07/2017" data-description="Lorem ipsum dolor sit amet, consectetur adipiscing elit...">
+
     <div class="item" data-id="04/07/2017" data-description="Design">
       <img class="con_borderImage" src="images/hp/timeline/3.jpg" alt="" />
+
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/c/cf/ZJU_China_HP_zbsmall.jpg" alt="" />
 
       <h2 class="timelineHead">JULY, 4</h2>
 
       <h2 class="timelineHead">JULY, 4</h2>
       <span>ZHOU BIAN...</span>
+
       <span>After determining our logo, we designed some derivative products such as postcard, key chain, badge, notebook, stickers, etc. We sincerely hope that these gifts can get everyone's favor during intercollegiate communication.</span>
 
     </div>
 
     </div>
  
  
 
     <div class="item" data-id="09/07/2017" data-description="The hall in ZJSTM">
 
     <div class="item" data-id="09/07/2017" data-description="The hall in ZJSTM">
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/f/f6/ZJU_China_HP_kxy.jpg" alt="" />
+
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/2/28/ZJU_China_HP_kjg.jpg" alt="" />
 
       <h2 class="timelineHead">JULY, 9</h2>
 
       <h2 class="timelineHead">JULY, 9</h2>
       <span>We held a "garden party" in the Zhejiang Science and Technology Museum, the children who joined in it said: "I've learned lots of scientific knowledge in this activity." "I found it interesting. Give the thumbs up."</span>
+
       <span>We held a "garden party" in the Zhejiang Science and Technology Museum. The children who joined in it said: "I've learned lots of scientific knowledge in this activity." "I found it interesting. Give the thumbs up."</span>
 
     </div>
 
     </div>
 
      
 
      
 
      
 
      
     <div class="item" data-id="13/07/2017" data-description="yao jian ju">
+
     <div class="item" data-id="13/07/2017" data-description="State Food and Drug Administration">
 
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/1/13/ZJU_China_HP_yjj.jpg" alt="" />
 
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/1/13/ZJU_China_HP_yjj.jpg" alt="" />
 
       <h2 class="timelineHead">JULY, 13</h2>
 
       <h2 class="timelineHead">JULY, 13</h2>
       <span>yaojianju</span>
+
       <span>"I do believe that such cutting edge technology can do great benefits to people once it grows fully mature. But we have to make sure it's safe before we take a closer look at its effectiveness," one of the officials said.</span>
 
     </div>
 
     </div>
 
      
 
      
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     <div class="item" data-id="19/07/2017" data-description="National Demonstration Center for Experimental Biology Education in ZJU">
 
     <div class="item" data-id="19/07/2017" data-description="National Demonstration Center for Experimental Biology Education in ZJU">
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/2/28/ZJU_China_HP_kjg.jpg" alt="" />
+
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/f/f6/ZJU_China_HP_kxy.jpg" alt="" />
 
       <h2 class="timelineHead">JULY, 19</h2>
 
       <h2 class="timelineHead">JULY, 19</h2>
       <span>"Wow, What is this? How shall I use it?"
+
       <span>National Demonstration Center for Experimental Biology Education in ZJU<br>"Wow, What is this? How shall I use it?"<br>"Welcome to our lab, let me guide you!"</span>
"Welcome to our lab, let me guide you!"
+
</span>
+
 
     </div>
 
     </div>
 
      
 
      
 
     <div class="item" data-id="20/07/2017" data-description="Software">
 
     <div class="item" data-id="20/07/2017" data-description="Software">
       <img class="con_borderImage" src="images/hp/timeline/7.jpg" alt="" />
+
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/1/13/ZJU_China_HP_Game.png" alt="" />
 
       <h2 class="timelineHead">JULY, 20</h2>
 
       <h2 class="timelineHead">JULY, 20</h2>
       <span>Can Trichoderma defend your plant from dangerous? It depends on you.  
+
       <span>Can Trichoderma defend your plant from dangerous? It depends on you. Play our computer game and see who's the winner.</span>
Play our computer game and see who's the winner.
+
</span>
+
 
     </div>
 
     </div>
 
      
 
      
     <div class="item" data-id="23/08/2017" data-description="yan cao gong si">
+
     <div class="item" data-id="23/08/2017" data-description="Hunan Province">
 
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/5/59/ZJU_China_HP_ycgs.jpg" alt="" />
 
       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/5/59/ZJU_China_HP_ycgs.jpg" alt="" />
 
       <h2 class="timelineHead">AUGUST, 23</h2>
 
       <h2 class="timelineHead">AUGUST, 23</h2>
       <span>yan cao gong si</span>
+
       <span>By visiting the tobacco field as well as communicating with the officials in the tobacco company, we got useful suggestions and inspirations. Then we designed our device and modified our "wetlab" work.</span>
 +
    </div>
 +
 
 +
    <div class="item" data-id="25/08/2017" data-description="Northeastern University">
 +
        <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/5/5d/ZJU_China_HP_DBDX.jpg" alt="" />
 +
        <h2 class="timelineHead">AUGUST, 25</h2>
 +
        <span>We had a freewheeling talk with the iGEM team of Northeastern University, because of this talk,  we later had opportunities to collaborate with them in experiments.</span>
 
     </div>
 
     </div>
 
      
 
      
 
     <div class="item" data-id="26/08/2017" data-description="CCiC">
 
     <div class="item" data-id="26/08/2017" data-description="CCiC">
       <img class="con_borderImage" src="images/hp/timeline/9.jpg" alt="" />
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       <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/5/52/ZJU_China_HP_CCIC.jpg" alt="" />
 
       <h2 class="timelineHead">AUGHST, 26</h2>
 
       <h2 class="timelineHead">AUGHST, 26</h2>
       <span>CCiC</span>
+
       <span>Many suggestions that we received from other teams are of value, so we further improved our design after coming back. Besides, we built close cooperation with UESTC-China since we both used the tobacco.</span>
 +
    </div>
 +
 
 +
    <div class="item" data-id="07/10/2017" data-description="BTGEM Summit">
 +
        <img class="con_borderImage" src="https://static.igem.org/mediawiki/2017/d/db/ZJU_China_HP_BTGEM.jpg" alt="" />
 +
        <h2 class="timelineHead">OCTOBER, 7</h2>
 +
        <span>We trained ourselves through telling others our story, and they fed back what they were interested in or what they could not figure out, and all these information helped us discover many defects in our presentation.</span>
 
     </div>
 
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   <h1 id="introduction" class="page-header ArticleHead GreenAH">Human Practice</h1>
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   <h1 id="summary" class="page-header ArticleHead GreenAH">Human Practice Summary</h1>
  <p class="PP">The InterLab study is developed by the Measurement Committee. Since synthetic biology, also been called engineering biology, is based on engineering thinking and methods, it values the reliability and repeatability of measurements. The InterLab study, involving teams and labs around the world,takes green fluorescent protein (gfp) as an example after one strict, detailed protocol to ensure the common, comparable units for measuring, which hopes to test the robustness by comparing the results from different labs.</p>
+
          <p class="PP">We engaged with stakeholders as well as experts,and did field study etc., which as a whole helped us to design and improve our project.</p>
  <p class="PP">This is the Fourth Measurement InterLab and our team participates in it. The InterLab this year tries to establish a GFP measurement protocol based on engineering principles, find out the similarity between fluorescence measured around the world and make gene expression more precise and reliable through tests of some RBS devices (BCDs). We are provided with materials for standard curves together with positive control, negative control and devices with different promoters and RBSs. Strictly following the requirements and protocol, we have obtained the relevant datas.</p>
+
          <p class="PP">We consider the safety problem and interviewed the officials about bio-safety legislation. New designs and changes occurred after we made a trip to the tobacco company. Also, we made a lot of connection with public, especially teenagers, through activities like garden party, open house day, project-related computer game and so on. What's more, we joined in some meetings with other participants.</p>
            
+
          <p class="PP">We saw the current condition and problem, and made dialogues with professors and common people to got useful advice. Then, we made new things(like our VOC device) and confirmed one direction of our project. Through the project process, human practice helped us throw light on public issues and make efforts to solve the practical problem.</p>
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              <br><br>
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          <a class="CuteButton GreenCB" href="https://2017.igem.org/Team:ZJU-China/HP/Silver">More HP About Silver...</a>
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          <br><br><br>
 +
          <a class="CuteButton YellowCB" href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">More HP About Gold...</a>
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       <li><a href="#introduction">Introduction</a></li>
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       <li><a href="#summary">Summary</a></li>
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        <a href="#methods">Methods&Materials</a>
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          <ul class="nav">
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            <li><a href="#transformation">Transformation</a></li>
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            <li><a href="#colonies">Colonies Selection</a></li>
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            <li><a href="#calibration">Calibration</a></li>
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            <li><a href="#cellmeasurement">Cell Measurement</a></li>
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        <a href="#results">Results</a>
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            <li><a href="#OD600">OD600</a></li>
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            <li><a href="#fluorescein">Fluorescein</a></li>
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            <li><a href="#discussion">Discussion</a></li>
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            <li><a href="#reflection">Reflection</a></li>
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        <a href="#feedback">Feedback</a>
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          <li><a href="#advantages">Advantages</a></li>
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          <li><a href="#improvements">Improvements</a></li>
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          <!--  <i class="fa fa-arrow-up nav_icon"></i> -->
    ↑ UP ↑
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Latest revision as of 02:09, 2 November 2017

HP

MAY, 23

When your team is on the stage of brainstorming, are you bothered with looking for the former iGEM teams' information? Take out your smart phone and try our Mini Program.

MAY, 28

"We just built up a iGEM high school team, how shall we start?"
"Don't worry! We can share our experience with you."

JULY, 4

After determining our logo, we designed some derivative products such as postcard, key chain, badge, notebook, stickers, etc. We sincerely hope that these gifts can get everyone's favor during intercollegiate communication.

JULY, 9

We held a "garden party" in the Zhejiang Science and Technology Museum. The children who joined in it said: "I've learned lots of scientific knowledge in this activity." "I found it interesting. Give the thumbs up."

JULY, 13

"I do believe that such cutting edge technology can do great benefits to people once it grows fully mature. But we have to make sure it's safe before we take a closer look at its effectiveness," one of the officials said.

JULY, 19

National Demonstration Center for Experimental Biology Education in ZJU
"Wow, What is this? How shall I use it?"
"Welcome to our lab, let me guide you!"

JULY, 20

Can Trichoderma defend your plant from dangerous? It depends on you. Play our computer game and see who's the winner.

AUGUST, 23

By visiting the tobacco field as well as communicating with the officials in the tobacco company, we got useful suggestions and inspirations. Then we designed our device and modified our "wetlab" work.

AUGUST, 25

We had a freewheeling talk with the iGEM team of Northeastern University, because of this talk, we later had opportunities to collaborate with them in experiments.

AUGHST, 26

Many suggestions that we received from other teams are of value, so we further improved our design after coming back. Besides, we built close cooperation with UESTC-China since we both used the tobacco.

OCTOBER, 7

We trained ourselves through telling others our story, and they fed back what they were interested in or what they could not figure out, and all these information helped us discover many defects in our presentation.

Human Practice Summary

We engaged with stakeholders as well as experts,and did field study etc., which as a whole helped us to design and improve our project.

We consider the safety problem and interviewed the officials about bio-safety legislation. New designs and changes occurred after we made a trip to the tobacco company. Also, we made a lot of connection with public, especially teenagers, through activities like garden party, open house day, project-related computer game and so on. What's more, we joined in some meetings with other participants.

We saw the current condition and problem, and made dialogues with professors and common people to got useful advice. Then, we made new things(like our VOC device) and confirmed one direction of our project. Through the project process, human practice helped us throw light on public issues and make efforts to solve the practical problem.