Difference between revisions of "Team:ZJU-China/Parts"
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<a href="#" class="dropdown-toggle link" data-toggle="dropdown">Overview<b class="caret"></b></a> | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Overview<b class="caret"></b></a> | ||
<ul class="dropdown-menu "> | <ul class="dropdown-menu "> | ||
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/Overview"> | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Overview">Description</a></li> |
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Demonstrate">Demonstrate</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Applied_Design">Applied Design</a></li> | ||
<li><a href="https://2017.igem.org/Team:ZJU-China/Achievements">Achievements</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/Achievements">Achievements</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Improve">Improve Parts</a></li> | ||
<li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">InterLab</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">InterLab</a></li> | ||
| − | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
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<a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a> | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a> | ||
<ul class="dropdown-menu "> | <ul class="dropdown-menu "> | ||
| − | |||
<li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li> | ||
<li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li> | ||
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/st">Signal Transduction</a></li> | + | <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/st">Chemical Signal Transduction</a></li> |
| − | <li><a | + | <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/mt">Medium Wave Transduction</a></li> |
| − | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/Downstream">Downstream</a></li> | |
| − | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/conclusion">Conclusions</a></li> | |
| − | + | ||
<li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Protocols">Protocols</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li class="m_nav_item dropdown" > | <li class="m_nav_item dropdown" > | ||
| − | <a href="#" class="dropdown-toggle link" data-toggle="dropdown"> | + | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Model<b class="caret"></b></a> |
<ul class="dropdown-menu "> | <ul class="dropdown-menu "> | ||
<!--<li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>--> | <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>--> | ||
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/Model"> | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Model">VOC analysis</a></li> |
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/Model/ | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Model/Coculture">Coculture</a></li> |
</ul> | </ul> | ||
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<ul class="dropdown-menu "> | <ul class="dropdown-menu "> | ||
<li><a href="https://2017.igem.org/Team:ZJU-China/Parts">All Parts</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/Parts">All Parts</a></li> | ||
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/ | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Basic Parts</a></li> |
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/ | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Composite Parts</a></li> |
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/ | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Part Collection</a></li> |
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware">Hardware</a></li> | + | <li class="m_nav_item dropdown" > |
| + | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Hardware<b class="caret"></b></a> | ||
| + | <ul class="dropdown-menu "> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware">Overview</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Device">Device</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Improvements">Improvements</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/MediumWave">Medium Wave</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
<li class="m_nav_item dropdown" > | <li class="m_nav_item dropdown" > | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<li class="m_nav_item dropdown"> | <li class="m_nav_item dropdown"> | ||
<a href="#" class="dropdown-toggle link" data-toggle="dropdown">HP<b class="caret"></b></a> | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">HP<b class="caret"></b></a> | ||
<ul class="dropdown-menu "> | <ul class="dropdown-menu "> | ||
| + | |||
<li><a href="https://2017.igem.org/Team:ZJU-China/Human_Practices">Summary</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/Human_Practices">Summary</a></li> | ||
<li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li> | <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li> | ||
| − | <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold</a></li> | + | <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold Integrated</a></li> |
| + | <li><a href="https://2017.igem.org/Team:ZJU-China/Engagement">Engagement</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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</ul> | </ul> | ||
</div> | </div> | ||
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</div> | </div> | ||
| + | <p></p> | ||
| − | + | <div style="width: 100%" class="container zjuContent"> | |
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<div class="col-md-3"></div> | <div class="col-md-3"></div> | ||
<div class="col-md-9" role="main"> | <div class="col-md-9" role="main"> | ||
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<div style="height: 50px;"></div> | <div style="height: 50px;"></div> | ||
| − | <h1 id="introduction" class="page-header ArticleHead GreenAH">Parts | + | <h1 id="introduction" class="page-header ArticleHead GreenAH">Parts</h1> |
<p class="PP">Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.</p> | <p class="PP">Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.</p> | ||
<p class="PP">We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.</p> | <p class="PP">We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.</p> | ||
| Line 533: | Line 530: | ||
<!-- Table --> | <!-- Table --> | ||
| − | <table id="partTable" class="table | + | <table id="partTable" class="table"> |
<tr> | <tr> | ||
| − | <th>Name</th> | + | <th class="yellowTable">Name</th> |
| − | <th>Type</th> | + | <th class="yellowTable">Type</th> |
| − | <th>Description</th> | + | <th class="yellowTable">Description</th> |
| − | <th>Designer</th> | + | <th class="yellowTable">Designer</th> |
| − | <th>Length(bp)</th> | + | <th class="yellowTable">Length(bp)</th> |
| − | <th>Submitted</th> | + | <th class="yellowTable">Submitted</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207000</th> | + | <th class="grayTable">BBa_K2207000</th> |
<td>Device</td> | <td>Device</td> | ||
<td>H3-RP27 expression system</td> | <td>H3-RP27 expression system</td> | ||
| Line 552: | Line 549: | ||
<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
| − | <th>BBa_K2207001</th> | + | <tr> |
| + | <th class="grayTable">BBa_K2207001</th> | ||
<td>Promoters</td> | <td>Promoters</td> | ||
<td>Hex promoter</td> | <td>Hex promoter</td> | ||
| Line 558: | Line 556: | ||
<td>3317</td> | <td>3317</td> | ||
<td></td> | <td></td> | ||
| − | + | </tr> | |
<tr> | <tr> | ||
| − | <th>BBa_K2207002</th> | + | <th class="grayTable">BBa_K2207002</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>Ech42 promoter</td> | <td>Ech42 promoter</td> | ||
| Line 569: | Line 567: | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207003</th> | + | <th class="grayTable">BBa_K2207003</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System I SOD-Tubulin dobule promoter</td> | <td>Trichoderma HR System I SOD-Tubulin dobule promoter</td> | ||
| Line 579: | Line 577: | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207004</th> | + | <th class="grayTable">BBa_K2207004</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System II Ech42-H3 double promoter</td> | <td>Trichoderma HR System II Ech42-H3 double promoter</td> | ||
| Line 589: | Line 587: | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207005</th> | + | <th class="grayTable">BBa_K2207005</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System III L1-ADH1-RP27-L2</td> | <td>Trichoderma HR System III L1-ADH1-RP27-L2</td> | ||
| Line 598: | Line 596: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207006</th> | + | <th class="grayTable">BBa_K2207006</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System IV L3-ADH1-RP27-L4</td> | <td>Trichoderma HR System IV L3-ADH1-RP27-L4</td> | ||
| Line 608: | Line 606: | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207007</th> | + | <th class="grayTable">BBa_K2207007</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System V Homologous Binding SiteA</td> | <td>Trichoderma HR System V Homologous Binding SiteA</td> | ||
| Line 617: | Line 615: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207008</th> | + | <th class="grayTable">BBa_K2207008</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System VI Homologous Binding SiteB</td> | <td>Trichoderma HR System VI Homologous Binding SiteB</td> | ||
| Line 627: | Line 625: | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207009</th> | + | <th class="grayTable">BBa_K2207009</th> |
<td>Coding Sequences</td> | <td>Coding Sequences</td> | ||
<td>PhlF transcription factor</td> | <td>PhlF transcription factor</td> | ||
| Line 636: | Line 634: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207010</th> | + | <th class="grayTable">BBa_K2207010</th> |
<td>Other</td> | <td>Other</td> | ||
<td>Phl Operator</td> | <td>Phl Operator</td> | ||
| Line 646: | Line 644: | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207011</th> | + | <th class="grayTable">BBa_K2207011</th> |
<td>Protein Generators</td> | <td>Protein Generators</td> | ||
<td>Phl Operator(BBa_K2207010) mRFP</td> | <td>Phl Operator(BBa_K2207010) mRFP</td> | ||
| Line 655: | Line 653: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207012</th> | + | <th class="grayTable">BBa_K2207012</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>DAPG sensor(PhlOF)</td> | <td>DAPG sensor(PhlOF)</td> | ||
| Line 664: | Line 662: | ||
<tr> | <tr> | ||
| − | <th> | + | <th class="grayTable">BBa_K2207998</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>2,4-DAPG PhlABCD Cluster</td> | <td>2,4-DAPG PhlABCD Cluster</td> | ||
<td>Junmin Qian</td> | <td>Junmin Qian</td> | ||
<td>4332</td> | <td>4332</td> | ||
| − | <td> | + | <td> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207014</th> | + | <th class="grayTable">BBa_K2207014</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlAC enzyme</td> | <td>PhlAC enzyme</td> | ||
<td>Junmin Qian</td> | <td>Junmin Qian</td> | ||
<td>5025</td> | <td>5025</td> | ||
| − | <td></td> | + | <td> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207015</th> | + | <th class="grayTable">BBa_K2207015</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlBD enzyme</td> | <td>PhlBD enzyme</td> | ||
| Line 690: | Line 688: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207016</th> | + | <th class="grayTable">BBa_K2207016</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlAB enzyme</td> | <td>PhlAB enzyme</td> | ||
| Line 698: | Line 696: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207017</th> | + | <th class="grayTable">BBa_K2207017</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlCD enzyme</td> | <td>PhlCD enzyme</td> | ||
| Line 706: | Line 704: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207018</th> | + | <th class="grayTable">BBa_K2207018</th> |
<td>Devices</td> | <td>Devices</td> | ||
<td>GPD-CYC1 expression system</td> | <td>GPD-CYC1 expression system</td> | ||
| Line 714: | Line 712: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207019</th> | + | <th class="grayTable">BBa_K2207019</th> |
<td>Translational units</td> | <td>Translational units</td> | ||
<td>TRPV1-Ferritin</td> | <td>TRPV1-Ferritin</td> | ||
| Line 722: | Line 720: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207020</th> | + | <th class="grayTable">BBa_K2207020</th> |
<td>Translational units</td> | <td>Translational units</td> | ||
<td>Ferritin</td> | <td>Ferritin</td> | ||
| Line 731: | Line 729: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207021</th> | + | <th class="grayTable">BBa_K2207021</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>Calcineurin-dependent response element (CDRE)</td> | <td>Calcineurin-dependent response element (CDRE)</td> | ||
| Line 738: | Line 736: | ||
<td>✔</td> | <td>✔</td> | ||
</tr> | </tr> | ||
| + | |||
<tr> | <tr> | ||
| − | <th>BBa_K2207022</th> | + | <th class="grayTable">BBa_K2207022</th> |
<td>Protein Generators</td> | <td>Protein Generators</td> | ||
| − | <td>CDRE(BBa_K2207021) mrfp</td> | + | <td>CDRE(BBa_K2207021) oligoA-mrfp</td> |
<td>Shisheng Li</td> | <td>Shisheng Li</td> | ||
<td>1186</td> | <td>1186</td> | ||
<td>✔</td> | <td>✔</td> | ||
</tr> | </tr> | ||
| + | |||
<tr> | <tr> | ||
| − | <th>BBa_K2207023</th> | + | <th class="grayTable">BBa_K2207023</th> |
<td>Coding Sequences</td> | <td>Coding Sequences</td> | ||
<td>Mature serine protein from Paecilomyces lilacinus</td> | <td>Mature serine protein from Paecilomyces lilacinus</td> | ||
| Line 755: | Line 755: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207024</th> | + | <th class="grayTable">BBa_K2207024</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant1</td> | <td>T7-mutant1</td> | ||
| Line 764: | Line 764: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207025</th> | + | <th class="grayTable">BBa_K2207025</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant2</td> | <td>T7-mutant2</td> | ||
| Line 772: | Line 772: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207026</th> | + | <th class="grayTable">BBa_K2207026</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant3</td> | <td>T7-mutant3</td> | ||
| Line 780: | Line 780: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207027</th> | + | <th class="grayTable">BBa_K2207027</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant4</td> | <td>T7-mutant4</td> | ||
| Line 788: | Line 788: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207028</th> | + | <th class="grayTable">BBa_K2207028</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant5</td> | <td>T7-mutant5</td> | ||
| Line 796: | Line 796: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207029</th> | + | <th class="grayTable">BBa_K2207029</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant6</td> | <td>T7-mutant6</td> | ||
| Line 804: | Line 804: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207030</th> | + | <th class="grayTable">BBa_K2207030</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant7</td> | <td>T7-mutant7</td> | ||
| Line 812: | Line 812: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <th>BBa_K2207031</th> | + | <th class="grayTable">BBa_K2207031</th> |
<td>Coding Sequences</td> | <td>Coding Sequences</td> | ||
<td>PhlE efflux pump</td> | <td>PhlE efflux pump</td> | ||
| − | <td> | + | <td>Junming Qian</td> |
<td>1272</td> | <td>1272</td> | ||
<td>✔</td> | <td>✔</td> | ||
</tr> | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <th class="grayTable">BBa_K2207997</th> | ||
| + | <td>Composite Parts</td> | ||
| + | <td>2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator</td> | ||
| + | <td>Junming Qian</td> | ||
| + | <td>1272</td> | ||
| + | <td>✔</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <th class="grayTable">BBa_K2207036</th> | ||
| + | <td>Coding Sequencess</td> | ||
| + | <td>oligoA-mRFP</td> | ||
| + | <td>Shisheng Li</td> | ||
| + | <td>712</td> | ||
| + | <td>✔</td> | ||
| + | </tr> | ||
</table> | </table> | ||
| Line 825: | Line 843: | ||
<h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3> | <h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3> | ||
<p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p> | <p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p> | ||
| − | + | <p class="PP">We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Project/tp#overview">Click here</a> to see how we constructed these systems.</p> | |
| + | |||
| + | <h3 class="H3Head">Our calcium ion response promoter.</h3> | ||
| + | <p class="PP Retract"><strong>BBa_K2207021</strong></p> | ||
| + | <p class="PP">We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Click here</a> to see how we use this part.</p> | ||
| + | |||
| − | + | <h2 id="BestCompositeParts" class="H2Head">Best Composite Parts</h2> | |
<p class="PP Retract"><strong>BBa_K2207013</strong></p> | <p class="PP Retract"><strong>BBa_K2207013</strong></p> | ||
| − | + | <p class="PP">We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Click here</a> to see our fantastic work.</p> | |
<!--配DAPG基因簇图--> | <!--配DAPG基因簇图--> | ||
<h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2> | <h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2> | ||
<p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p> | <p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p> | ||
| − | <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.</p> | + | <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Click here</a> to see more details.</p> |
<!--配IP结果处理图--> | <!--配IP结果处理图--> | ||
| Line 844: | Line 867: | ||
<div class="col-md-3 disappear-on-top" role="complementary"> | <div class="col-md-3 disappear-on-top" role="complementary"> | ||
<nav style="position: fixed; top: 100px ; left:50px;" class="bs-docs-sidebar hidden-print hidden-xs hidden-sm"> | <nav style="position: fixed; top: 100px ; left:50px;" class="bs-docs-sidebar hidden-print hidden-xs hidden-sm"> | ||
| − | <ul class="nav bs-docs-sidenav"> | + | <ul class="nav bs-docs-sidenav shorterli"> |
<li><a href="#introduction">Introduction</a></li> | <li><a href="#introduction">Introduction</a></li> | ||
Latest revision as of 15:18, 1 November 2017
Parts
Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.
We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.
| Name | Type | Description | Designer | Length(bp) | Submitted |
|---|---|---|---|---|---|
| BBa_K2207000 | Device | H3-RP27 expression system | Qianjin Jiang | 2644 | |
| BBa_K2207001 | Promoters | Hex promoter | Junbo Yang | 3317 | |
| BBa_K2207002 | Promoters | Ech42 promoter | Junbo Yang | 3352 | |
| BBa_K2207003 | Composite Parts | Trichoderma HR System I SOD-Tubulin dobule promoter | Yihe Zhang | 3155 | ✔ |
| BBa_K2207004 | Composite Parts | Trichoderma HR System II Ech42-H3 double promoter | Yihe Zhang | 4845 | ✔ |
| BBa_K2207005 | Composite Parts | Trichoderma HR System III L1-ADH1-RP27-L2 | Yihe Zhang | 903 | ✔ |
| BBa_K2207006 | Composite Parts | Trichoderma HR System IV L3-ADH1-RP27-L4 | Yihe Zhang | 903 | ✔ |
| BBa_K2207007 | Composite Parts | Trichoderma HR System V Homologous Binding SiteA | Yihe Zhang | 1676 | ✔ |
| BBa_K2207008 | Composite Parts | Trichoderma HR System VI Homologous Binding SiteB | Yihe Zhang | 1650 | ✔ |
| BBa_K2207009 | Coding Sequences | PhlF transcription factor | Qianjin Jiang | 627 | ✔ |
| BBa_K2207010 | Other | Phl Operator | Yuxing Chen | 825 | ✔ |
| BBa_K2207011 | Protein Generators | Phl Operator(BBa_K2207010) mRFP | Yuxing Chen | 1545 | |
| BBa_K2207012 | Composite Parts | DAPG sensor(PhlOF) | Qianjin Jiang | 1450 | |
| BBa_K2207998 | Composite Parts | 2,4-DAPG PhlABCD Cluster | Junmin Qian | 4332 | |
| BBa_K2207014 | Composite Parts | PhlAC enzyme | Junmin Qian | 5025 | |
| BBa_K2207015 | Composite Parts | PhlBD enzyme | Junmin Qian | 4212 | |
| BBa_K2207016 | Composite Parts | PhlAB enzyme | Junmin Qian | 5604 | |
| BBa_K2207017 | Composite Parts | PhlCD enzyme | Junmin Qian | 4646 | |
| BBa_K2207018 | Devices | GPD-CYC1 expression system | Shisheng Li | 382 | |
| BBa_K2207019 | Translational units | TRPV1-Ferritin | Cheng Shen | 4853 | |
| BBa_K2207020 | Translational units | Ferritin | Cheng Shen | 1173 | |
| BBa_K2207021 | Promoters | Calcineurin-dependent response element (CDRE) | Shisheng Li | 474 | ✔ |
| BBa_K2207022 | Protein Generators | CDRE(BBa_K2207021) oligoA-mrfp | Shisheng Li | 1186 | ✔ |
| BBa_K2207023 | Coding Sequences | Mature serine protein from Paecilomyces lilacinus | Zifan Xie | 882 | ✔ |
| BBa_K2207024 | Promoters | T7-mutant1 | Yuxing Chen | 42 | ✔ |
| BBa_K2207025 | Promoters | T7-mutant2 | Yuxing Chen | 42 | ✔ |
| BBa_K2207026 | Promoters | T7-mutant3 | Yuxing Chen | 42 | ✔ |
| BBa_K2207027 | Promoters | T7-mutant4 | Yuxing Chen | 42 | ✔ |
| BBa_K2207028 | Promoters | T7-mutant5 | Yuxing Chen | 42 | ✔ |
| BBa_K2207029 | Promoters | T7-mutant6 | Yuxing Chen | 42 | ✔ |
| BBa_K2207030 | Promoters | T7-mutant7 | Yuxing Chen | 42 | ✔ |
| BBa_K2207031 | Coding Sequences | PhlE efflux pump | Junming Qian | 1272 | ✔ |
| BBa_K2207997 | Composite Parts | 2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator | Junming Qian | 1272 | ✔ |
| BBa_K2207036 | Coding Sequencess | oligoA-mRFP | Shisheng Li | 712 | ✔ |
Favorite Parts!
Our new Basic Parts in Trichoderma spp.
BBa_K2207003 ~ BBa_K2207008
We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.
Our calcium ion response promoter.
BBa_K2207021
We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. Click here to see how we use this part.
Best Composite Parts
BBa_K2207013
We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.
Improve T7 promoters
BBa_K2207024 ~ BBa_K2207030
T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.
