Difference between revisions of "Team:ZJU-China/Parts"

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}
  
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.blueTable
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.grayTable
 
{
 
{
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     background-color: #979797 !important;
 +
    color: black !important;
 
}
 
}
  
 
.yellowTable
 
.yellowTable
 
{
 
{
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     color: #CD9B1D;
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   <!-- Table -->
 
   <!-- Table -->
  
−
   <table id="partTable" class="table tableNice">
+
   <table id="partTable" class="table">
 
   <tr>
 
   <tr>
−
<th>Name</th>
+
<th class="yellowTable">Name</th>
−
<th>Type</th>
+
<th class="yellowTable">Type</th>
−
<th>Description</th>
+
<th class="yellowTable">Description</th>
−
<th>Designer</th>
+
<th class="yellowTable">Designer</th>
−
<th>Length(bp)</th>
+
<th class="yellowTable">Length(bp)</th>
−
<th>Submitted</th>
+
<th class="yellowTable">Submitted</th>
  
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
−
     <th>BBa_K2207000</th>
+
     <th class="grayTable">BBa_K2207000</th>
 
     <td>Device</td>
 
     <td>Device</td>
 
     <td>H3-RP27 expression system</td>
 
     <td>H3-RP27 expression system</td>
Line 545: Line 547:
 
</tr>
 
</tr>
 
       <tr>
 
       <tr>
−
       <th>BBa_K2207001</th>
+
       <th class="grayTable">BBa_K2207001</th>
 
       <td>Promoters</td>
 
       <td>Promoters</td>
 
       <td>Hex promoter</td>
 
       <td>Hex promoter</td>
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       </tr>
 
       </tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207002</th>
+
     <th class="grayTable">BBa_K2207002</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>Ech42 promoter</td>
 
     <td>Ech42 promoter</td>
Line 562: Line 564:
  
 
<tr>
 
<tr>
−
     <th>BBa_K2207003</th>
+
     <th class="grayTable">BBa_K2207003</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>Trichoderma HR System I SOD-Tubulin dobule promoter</td>
 
     <td>Trichoderma HR System I SOD-Tubulin dobule promoter</td>
Line 572: Line 574:
  
 
<tr>
 
<tr>
−
     <th>BBa_K2207004</th>
+
     <th class="grayTable">BBa_K2207004</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>Trichoderma HR System II Ech42-H3 double promoter</td>
 
     <td>Trichoderma HR System II Ech42-H3 double promoter</td>
Line 582: Line 584:
  
 
<tr>
 
<tr>
−
     <th>BBa_K2207005</th>
+
     <th class="grayTable">BBa_K2207005</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>Trichoderma HR System III L1-ADH1-RP27-L2</td>
 
     <td>Trichoderma HR System III L1-ADH1-RP27-L2</td>
Line 591: Line 593:
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207006</th>
+
     <th class="grayTable">BBa_K2207006</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>Trichoderma HR System IV L3-ADH1-RP27-L4</td>
 
     <td>Trichoderma HR System IV L3-ADH1-RP27-L4</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207007</th>
+
     <th class="grayTable">BBa_K2207007</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>Trichoderma HR System V Homologous Binding SiteA</td>
 
     <td>Trichoderma HR System V Homologous Binding SiteA</td>
Line 610: Line 612:
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207008</th>
+
     <th class="grayTable">BBa_K2207008</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>Trichoderma HR System VI Homologous Binding SiteB</td>
 
     <td>Trichoderma HR System VI Homologous Binding SiteB</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207009</th>
+
     <th class="grayTable">BBa_K2207009</th>
 
     <td>Coding Sequences</td>
 
     <td>Coding Sequences</td>
 
     <td>PhlF transcription factor</td>
 
     <td>PhlF transcription factor</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207010</th>
+
     <th class="grayTable">BBa_K2207010</th>
 
     <td>Other</td>
 
     <td>Other</td>
 
     <td>Phl Operator</td>
 
     <td>Phl Operator</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207011</th>
+
     <th class="grayTable">BBa_K2207011</th>
 
     <td>Protein Generators</td>
 
     <td>Protein Generators</td>
 
     <td>Phl Operator(BBa_K2207010) mRFP</td>
 
     <td>Phl Operator(BBa_K2207010) mRFP</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207012</th>
+
     <th class="grayTable">BBa_K2207012</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>DAPG sensor(PhlOF)</td>
 
     <td>DAPG sensor(PhlOF)</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207998</th>
+
     <th class="grayTable">BBa_K2207998</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>2,4-DAPG PhlABCD Cluster</td>
 
     <td>2,4-DAPG PhlABCD Cluster</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207014</th>
+
     <th class="grayTable">BBa_K2207014</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>PhlAC enzyme</td>
 
     <td>PhlAC enzyme</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207015</th>
+
     <th class="grayTable">BBa_K2207015</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>PhlBD enzyme</td>
 
     <td>PhlBD enzyme</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207016</th>
+
     <th class="grayTable">BBa_K2207016</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>PhlAB enzyme</td>
 
     <td>PhlAB enzyme</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207017</th>
+
     <th class="grayTable">BBa_K2207017</th>
 
     <td>Composite Parts</td>
 
     <td>Composite Parts</td>
 
     <td>PhlCD enzyme</td>
 
     <td>PhlCD enzyme</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207018</th>
+
     <th class="grayTable">BBa_K2207018</th>
 
     <td>Devices</td>
 
     <td>Devices</td>
 
     <td>GPD-CYC1 expression system</td>
 
     <td>GPD-CYC1 expression system</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207019</th>
+
     <th class="grayTable">BBa_K2207019</th>
 
     <td>Translational units</td>
 
     <td>Translational units</td>
 
     <td>TRPV1-Ferritin</td>
 
     <td>TRPV1-Ferritin</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207020</th>
+
     <th class="grayTable">BBa_K2207020</th>
 
     <td>Translational units</td>
 
     <td>Translational units</td>
 
     <td>Ferritin</td>
 
     <td>Ferritin</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207021</th>
+
     <th class="grayTable">BBa_K2207021</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>Calcineurin-dependent response element (CDRE)</td>
 
     <td>Calcineurin-dependent response element (CDRE)</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207022</th>
+
     <th class="grayTable">BBa_K2207022</th>
 
     <td>Protein Generators</td>
 
     <td>Protein Generators</td>
 
     <td>CDRE(BBa_K2207021) oligoA-mrfp</td>
 
     <td>CDRE(BBa_K2207021) oligoA-mrfp</td>
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<tr>
 
<tr>
−
     <th>BBa_K2207023</th>
+
     <th class="grayTable">BBa_K2207023</th>
 
     <td>Coding Sequences</td>
 
     <td>Coding Sequences</td>
 
     <td>Mature serine protein from Paecilomyces lilacinus</td>
 
     <td>Mature serine protein from Paecilomyces lilacinus</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207024</th>
+
     <th class="grayTable">BBa_K2207024</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant1</td>
 
     <td>T7-mutant1</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207025</th>
+
     <th class="grayTable">BBa_K2207025</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant2</td>
 
     <td>T7-mutant2</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207026</th>
+
     <th class="grayTable">BBa_K2207026</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant3</td>
 
     <td>T7-mutant3</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207027</th>
+
     <th class="grayTable">BBa_K2207027</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant4</td>
 
     <td>T7-mutant4</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207028</th>
+
     <th class="grayTable">BBa_K2207028</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant5</td>
 
     <td>T7-mutant5</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207029</th>
+
     <th class="grayTable">BBa_K2207029</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant6</td>
 
     <td>T7-mutant6</td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <th>BBa_K2207030</th>
+
     <th class="grayTable">BBa_K2207030</th>
 
     <td>Promoters</td>
 
     <td>Promoters</td>
 
     <td>T7-mutant7</td>
 
     <td>T7-mutant7</td>
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</tr>
 
</tr>
 
   <tr>
 
   <tr>
−
       <th>BBa_K2207031</th>
+
       <th class="grayTable">BBa_K2207031</th>
 
       <td>Coding Sequences</td>
 
       <td>Coding Sequences</td>
 
       <td>PhlE efflux pump</td>
 
       <td>PhlE efflux pump</td>
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       <tr>
 
       <tr>
−
           <th>BBa_K2207997</th>
+
           <th class="grayTable">BBa_K2207997</th>
 
           <td>Composite Parts</td>
 
           <td>Composite Parts</td>
 
           <td>2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator</td>
 
           <td>2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator</td>
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       <tr>
 
       <tr>
−
           <th>BBa_K2207036</th>
+
           <th class="grayTable">BBa_K2207036</th>
 
           <td>Coding Sequencess</td>
 
           <td>Coding Sequencess</td>
 
           <td>oligoA-mRFP</td>
 
           <td>oligoA-mRFP</td>
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           <h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3>
 
           <h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3>
 
           <p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p>
 
           <p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p>
−
           <p class="PP">We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate  these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) <a class="city" href="https://2017.igem.org/Team:ZJU-China/Project/tp#overview">Click here</a> to see how we constructed these systems.</p>
+
           <p class="PP">We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate  these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Project/tp#overview">Click here</a> to see how we constructed these systems.</p>
  
 
           <h3 class="H3Head">Our calcium ion response  promoter.</h3>
 
           <h3 class="H3Head">Our calcium ion response  promoter.</h3>
 
               <p class="PP Retract"><strong>BBa_K2207021</strong></p>
 
               <p class="PP Retract"><strong>BBa_K2207021</strong></p>
−
           <p class="PP">We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin.</p>
+
           <p class="PP">We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Click here</a> to see how we use this part.</p>
  
  
 
           <h2 id="BestCompositeParts" class="H2Head">Best Composite Parts</h2>
 
           <h2 id="BestCompositeParts" class="H2Head">Best Composite Parts</h2>
 
           <p class="PP Retract"><strong>BBa_K2207013</strong></p>
 
           <p class="PP Retract"><strong>BBa_K2207013</strong></p>
−
  <p class="PP">We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.</p>
+
          <p class="PP">We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Click here</a> to see our fantastic work.</p>
 
   <!--配DAPG基因簇图-->
 
   <!--配DAPG基因簇图-->
  
 
   <h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2>
 
   <h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2>
 
           <p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p>
 
           <p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p>
−
           <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.</p>
+
           <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Click here</a> to see more details.</p>
 
       <!--配IP结果处理图-->
 
       <!--配IP结果处理图-->
  

Revision as of 01:54, 29 October 2017

Parts

Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.

We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.

Name Type Description Designer Length(bp) Submitted
BBa_K2207000 Device H3-RP27 expression system Qianjin Jiang 2644
BBa_K2207001 Promoters Hex promoter Junbo Yang 3317
BBa_K2207002 Promoters Ech42 promoter Junbo Yang 3352
BBa_K2207003 Composite Parts Trichoderma HR System I SOD-Tubulin dobule promoter Yihe Zhang 3155 ✔
BBa_K2207004 Composite Parts Trichoderma HR System II Ech42-H3 double promoter Yihe Zhang 4845 ✔
BBa_K2207005 Composite Parts Trichoderma HR System III L1-ADH1-RP27-L2 Yihe Zhang 903 ✔
BBa_K2207006 Composite Parts Trichoderma HR System IV L3-ADH1-RP27-L4 Yihe Zhang 903 ✔
BBa_K2207007 Composite Parts Trichoderma HR System V Homologous Binding SiteA Yihe Zhang 1676 ✔
BBa_K2207008 Composite Parts Trichoderma HR System VI Homologous Binding SiteB Yihe Zhang 1650 ✔
BBa_K2207009 Coding Sequences PhlF transcription factor Qianjin Jiang 627 ✔
BBa_K2207010 Other Phl Operator Yuxing Chen 825 ✔
BBa_K2207011 Protein Generators Phl Operator(BBa_K2207010) mRFP Yuxing Chen 1545
BBa_K2207012 Composite Parts DAPG sensor(PhlOF) Qianjin Jiang 1450
BBa_K2207998 Composite Parts 2,4-DAPG PhlABCD Cluster Junmin Qian 4332
BBa_K2207014 Composite Parts PhlAC enzyme Junmin Qian 5025
BBa_K2207015 Composite Parts PhlBD enzyme Junmin Qian 4212
BBa_K2207016 Composite Parts PhlAB enzyme Junmin Qian 5604
BBa_K2207017 Composite Parts PhlCD enzyme Junmin Qian 4646
BBa_K2207018 Devices GPD-CYC1 expression system Shisheng Li 382
BBa_K2207019 Translational units TRPV1-Ferritin Cheng Shen 4853
BBa_K2207020 Translational units Ferritin Cheng Shen 1173
BBa_K2207021 Promoters Calcineurin-dependent response element (CDRE) Shisheng Li 474 ✔
BBa_K2207022 Protein Generators CDRE(BBa_K2207021) oligoA-mrfp Shisheng Li 1186 ✔
BBa_K2207023 Coding Sequences Mature serine protein from Paecilomyces lilacinus Zifan Xie 882 ✔
BBa_K2207024 Promoters T7-mutant1 Yuxing Chen 42 ✔
BBa_K2207025 Promoters T7-mutant2 Yuxing Chen 42 ✔
BBa_K2207026 Promoters T7-mutant3 Yuxing Chen 42 ✔
BBa_K2207027 Promoters T7-mutant4 Yuxing Chen 42 ✔
BBa_K2207028 Promoters T7-mutant5 Yuxing Chen 42 ✔
BBa_K2207029 Promoters T7-mutant6 Yuxing Chen 42 ✔
BBa_K2207030 Promoters T7-mutant7 Yuxing Chen 42 ✔
BBa_K2207031 Coding Sequences PhlE efflux pump Junming Qian 1272 ✔
BBa_K2207997 Composite Parts 2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator Junming Qian 1272 ✔
BBa_K2207036 Coding Sequencess oligoA-mRFP Shisheng Li 712 ✔

Favorite Parts!

Our new Basic Parts in Trichoderma spp.

BBa_K2207003 ~ BBa_K2207008

We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.

Our calcium ion response promoter.

BBa_K2207021

We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. Click here to see how we use this part.

Best Composite Parts

BBa_K2207013

We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.

Improve T7 promoters

BBa_K2207024 ~ BBa_K2207030

T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.


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