Difference between revisions of "Template:Example"

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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"><head>
 
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 
<meta name="generator" content="MediaWiki 1.16.5">
 
<link rel="shortcut icon" href="/favicon.ico">
 
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2014.igem.org (en)">
 
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:LMU-Munich&amp;action=creativecommons" rel="meta">
 
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/">
 
<link rel="alternate" type="application/atom+xml" title="2014.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom"> <title>Team:LMU-Munich - 2014.igem.org</title>
 
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@import "/wiki/skins/igem/main.css?270";
 
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<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270">
 
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<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
 
 
<script type="text/javascript" async="" src="http://www.google-analytics.com/plugins/ua/linkid.js"></script><script async="" src="//www.google-analytics.com/analytics.js"></script><script>
 
var skin="igem",
 
stylepath="/wiki/skins",
 
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 
wgArticlePath="/$1",
 
wgScriptPath="/wiki",
 
wgScriptExtension=".php",
 
wgScript="/wiki/index.php",
 
wgVariantArticlePath=false,
 
wgActionPaths={},
 
wgServer="http://2014.igem.org",
 
wgCanonicalNamespace="",
 
wgCanonicalSpecialPageName=false,
 
wgNamespaceNumber=0,
 
wgPageName="Team:LMU-Munich",
 
wgTitle="Team:LMU-Munich",
 
wgAction="view",
 
wgArticleId=1189,
 
wgIsArticle=true,
 
wgUserName=null,
 
wgUserGroups=null,
 
wgUserLanguage="en",
 
wgContentLanguage="en",
 
wgBreakFrames=false,
 
wgCurRevisionId=403769,
 
wgVersion="1.16.5",
 
wgEnableAPI=true,
 
wgEnableWriteAPI=true,
 
wgSeparatorTransformTable=["", ""],
 
wgDigitTransformTable=["", ""],
 
wgMainPageTitle="Main Page",
 
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2014.igem.org", "5": "2014.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 
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wgSiteName="2014.igem.org",
 
wgCategories=[],
 
wgMWSuggestTemplate="http://2014.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 
wgDBname="2014_igem_org",
 
wgSearchNamespaces=[0],
 
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 
wgRestrictionEdit=[],
 
wgRestrictionMove=[];
 
</script>               
 
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 
<!-- Head Scripts -->
 
<script src="/wiki/skins/common/ajax.js?270"></script>
 
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 
<!-- jQuery Javascript -->
 
<script type="text/javascript" src="/common/jquery-latest.min.js"></script>
 
<script type="text/javascript" src="/common/tablesorter/jquery.tablesorter.min.js"></script>
 
        <link rel="stylesheet" type="text/css" href="/common/tablesorter/themes/groupparts/style.css">
 
        <link rel="stylesheet" type="text/css" href="/common/table_styles.css">
 
        <link rel="stylesheet" type="text/css" href="/forum/forum_styles.css">
 
<script type="text/javascript" src="/forum/forum_scripts.js"></script>
 
<style type="text/css">.MathJax_Hover_Frame {border-radius: .25em; -webkit-border-radius: .25em; -moz-border-radius: .25em; -khtml-border-radius: .25em; box-shadow: 0px 0px 15px #83A; -webkit-box-shadow: 0px 0px 15px #83A; -moz-box-shadow: 0px 0px 15px #83A; -khtml-box-shadow: 0px 0px 15px #83A; border: 1px solid #A6D ! important; display: inline-block; position: absolute}
 
.MathJax_Menu_Button .MathJax_Hover_Arrow {position: absolute; cursor: pointer; display: inline-block; border: 2px solid #AAA; border-radius: 4px; -webkit-border-radius: 4px; -moz-border-radius: 4px; -khtml-border-radius: 4px; font-family: 'Courier New',Courier; font-size: 9px; color: #F0F0F0}
 
.MathJax_Menu_Button .MathJax_Hover_Arrow span {display: block; background-color: #AAA; border: 1px solid; border-radius: 3px; line-height: 0; padding: 4px}
 
.MathJax_Hover_Arrow:hover {color: white!important; border: 2px solid #CCC!important}
 
.MathJax_Hover_Arrow:hover span {background-color: #CCC!important}
 
</style><style type="text/css">#MathJax_About {position: fixed; left: 50%; width: auto; text-align: center; border: 3px outset; padding: 1em 2em; background-color: #DDDDDD; color: black; cursor: default; font-family: message-box; font-size: 120%; font-style: normal; text-indent: 0; text-transform: none; line-height: normal; letter-spacing: normal; word-spacing: normal; word-wrap: normal; white-space: nowrap; float: none; z-index: 201; border-radius: 15px; -webkit-border-radius: 15px; -moz-border-radius: 15px; -khtml-border-radius: 15px; box-shadow: 0px 10px 20px #808080; -webkit-box-shadow: 0px 10px 20px #808080; -moz-box-shadow: 0px 10px 20px #808080; -khtml-box-shadow: 0px 10px 20px #808080; filter: progid:DXImageTransform.Microsoft.dropshadow(OffX=2, OffY=2, Color='gray', Positive='true')}
 
#MathJax_About.MathJax_MousePost {outline: none}
 
.MathJax_Menu {position: absolute; background-color: white; color: black; width: auto; padding: 2px; border: 1px solid #CCCCCC; margin: 0; cursor: default; font: menu; text-align: left; text-indent: 0; text-transform: none; line-height: normal; letter-spacing: normal; word-spacing: normal; word-wrap: normal; white-space: nowrap; float: none; z-index: 201; box-shadow: 0px 10px 20px #808080; -webkit-box-shadow: 0px 10px 20px #808080; -moz-box-shadow: 0px 10px 20px #808080; -khtml-box-shadow: 0px 10px 20px #808080; filter: progid:DXImageTransform.Microsoft.dropshadow(OffX=2, OffY=2, Color='gray', Positive='true')}
 
.MathJax_MenuItem {padding: 2px 2em; background: transparent}
 
.MathJax_MenuArrow {position: absolute; right: .5em; padding-top: .25em; color: #666666; font-size: .75em}
 
.MathJax_MenuActive .MathJax_MenuArrow {color: white}
 
.MathJax_MenuArrow.RTL {left: .5em; right: auto}
 
.MathJax_MenuCheck {position: absolute; left: .7em}
 
.MathJax_MenuCheck.RTL {right: .7em; left: auto}
 
.MathJax_MenuRadioCheck {position: absolute; left: 1em}
 
.MathJax_MenuRadioCheck.RTL {right: 1em; left: auto}
 
.MathJax_MenuLabel {padding: 2px 2em 4px 1.33em; font-style: italic}
 
.MathJax_MenuRule {border-top: 1px solid #CCCCCC; margin: 4px 1px 0px}
 
.MathJax_MenuDisabled {color: GrayText}
 
.MathJax_MenuActive {background-color: Highlight; color: HighlightText}
 
.MathJax_MenuDisabled:focus, .MathJax_MenuLabel:focus {background-color: #E8E8E8}
 
.MathJax_ContextMenu:focus {outline: none}
 
.MathJax_ContextMenu .MathJax_MenuItem:focus {outline: none}
 
#MathJax_AboutClose {top: .2em; right: .2em}
 
.MathJax_Menu .MathJax_MenuClose {top: -10px; left: -10px}
 
.MathJax_MenuClose {position: absolute; cursor: pointer; display: inline-block; border: 2px solid #AAA; border-radius: 18px; -webkit-border-radius: 18px; -moz-border-radius: 18px; -khtml-border-radius: 18px; font-family: 'Courier New',Courier; font-size: 24px; color: #F0F0F0}
 
.MathJax_MenuClose span {display: block; background-color: #AAA; border: 1.5px solid; border-radius: 18px; -webkit-border-radius: 18px; -moz-border-radius: 18px; -khtml-border-radius: 18px; line-height: 0; padding: 8px 0 6px}
 
.MathJax_MenuClose:hover {color: white!important; border: 2px solid #CCC!important}
 
.MathJax_MenuClose:hover span {background-color: #CCC!important}
 
.MathJax_MenuClose:hover:focus {outline: none}
 
</style><style type="text/css">.MathJax_Preview .MJXf-math {color: inherit!important}
 
</style><style type="text/css">.MJX_Assistive_MathML {position: absolute!important; top: 0; left: 0; clip: rect(1px, 1px, 1px, 1px); padding: 1px 0 0 0!important; border: 0!important; height: 1px!important; width: 1px!important; overflow: hidden!important; display: block!important; -webkit-touch-callout: none; -webkit-user-select: none; -khtml-user-select: none; -moz-user-select: none; -ms-user-select: none; user-select: none}
 
.MJX_Assistive_MathML.MJX_Assistive_MathML_Block {width: 100%!important}
 
</style><style type="text/css">#MathJax_Zoom {position: absolute; background-color: #F0F0F0; overflow: auto; display: block; z-index: 301; padding: .5em; border: 1px solid black; margin: 0; font-weight: normal; font-style: normal; text-align: left; text-indent: 0; text-transform: none; line-height: normal; letter-spacing: normal; word-spacing: normal; word-wrap: normal; white-space: nowrap; float: none; -webkit-box-sizing: content-box; -moz-box-sizing: content-box; box-sizing: content-box; box-shadow: 5px 5px 15px #AAAAAA; -webkit-box-shadow: 5px 5px 15px #AAAAAA; -moz-box-shadow: 5px 5px 15px #AAAAAA; -khtml-box-shadow: 5px 5px 15px #AAAAAA; filter: progid:DXImageTransform.Microsoft.dropshadow(OffX=2, OffY=2, Color='gray', Positive='true')}
 
#MathJax_ZoomOverlay {position: absolute; left: 0; top: 0; z-index: 300; display: inline-block; width: 100%; height: 100%; border: 0; padding: 0; margin: 0; background-color: white; opacity: 0; filter: alpha(opacity=0)}
 
#MathJax_ZoomFrame {position: relative; display: inline-block; height: 0; width: 0}
 
#MathJax_ZoomEventTrap {position: absolute; left: 0; top: 0; z-index: 302; display: inline-block; border: 0; padding: 0; margin: 0; background-color: white; opacity: 0; filter: alpha(opacity=0)}
 
</style><style type="text/css">.MathJax_Preview {color: #888}
 
#MathJax_Message {position: fixed; left: 1em; bottom: 1.5em; background-color: #E6E6E6; border: 1px solid #959595; margin: 0px; padding: 2px 8px; z-index: 102; color: black; font-size: 80%; width: auto; white-space: nowrap}
 
#MathJax_MSIE_Frame {position: absolute; top: 0; left: 0; width: 0px; z-index: 101; border: 0px; margin: 0px; padding: 0px}
 
.MathJax_Error {color: #CC0000; font-style: italic}
 
</style><style type="text/css">.MJXp-script {font-size: .8em}
 
.MJXp-right {-webkit-transform-origin: right; -moz-transform-origin: right; -ms-transform-origin: right; -o-transform-origin: right; transform-origin: right}
 
.MJXp-bold {font-weight: bold}
 
.MJXp-italic {font-style: italic}
 
.MJXp-scr {font-family: MathJax_Script,'Times New Roman',Times,STIXGeneral,serif}
 
.MJXp-frak {font-family: MathJax_Fraktur,'Times New Roman',Times,STIXGeneral,serif}
 
.MJXp-sf {font-family: MathJax_SansSerif,'Times New Roman',Times,STIXGeneral,serif}
 
.MJXp-cal {font-family: MathJax_Caligraphic,'Times New Roman',Times,STIXGeneral,serif}
 
.MJXp-mono {font-family: MathJax_Typewriter,'Times New Roman',Times,STIXGeneral,serif}
 
.MJXp-largeop {font-size: 150%}
 
.MJXp-largeop.MJXp-int {vertical-align: -.2em}
 
.MJXp-math {display: inline-block; line-height: 1.2; text-indent: 0; font-family: 'Times New Roman',Times,STIXGeneral,serif; white-space: nowrap; border-collapse: collapse}
 
.MJXp-display {display: block; text-align: center; margin: 1em 0}
 
.MJXp-math span {display: inline-block}
 
.MJXp-box {display: block!important; text-align: center}
 
.MJXp-box:after {content: " "}
 
.MJXp-rule {display: block!important; margin-top: .1em}
 
.MJXp-char {display: block!important}
 
.MJXp-mo {margin: 0 .15em}
 
.MJXp-mfrac {margin: 0 .125em; vertical-align: .25em}
 
.MJXp-denom {display: inline-table!important; width: 100%}
 
.MJXp-denom > * {display: table-row!important}
 
.MJXp-surd {vertical-align: top}
 
.MJXp-surd > * {display: block!important}
 
.MJXp-script-box > *  {display: table!important; height: 50%}
 
.MJXp-script-box > * > * {display: table-cell!important; vertical-align: top}
 
.MJXp-script-box > *:last-child > * {vertical-align: bottom}
 
.MJXp-script-box > * > * > * {display: block!important}
 
.MJXp-mphantom {visibility: hidden}
 
.MJXp-munderover {display: inline-table!important}
 
.MJXp-over {display: inline-block!important; text-align: center}
 
.MJXp-over > * {display: block!important}
 
.MJXp-munderover > * {display: table-row!important}
 
.MJXp-mtable {vertical-align: .25em; margin: 0 .125em}
 
.MJXp-mtable > * {display: inline-table!important; vertical-align: middle}
 
.MJXp-mtr {display: table-row!important}
 
.MJXp-mtd {display: table-cell!important; text-align: center; padding: .5em 0 0 .5em}
 
.MJXp-mtr > .MJXp-mtd:first-child {padding-left: 0}
 
.MJXp-mtr:first-child > .MJXp-mtd {padding-top: 0}
 
.MJXp-mlabeledtr {display: table-row!important}
 
.MJXp-mlabeledtr > .MJXp-mtd:first-child {padding-left: 0}
 
.MJXp-mlabeledtr:first-child > .MJXp-mtd {padding-top: 0}
 
.MJXp-merror {background-color: #FFFF88; color: #CC0000; border: 1px solid #CC0000; padding: 1px 3px; font-style: normal; font-size: 90%}
 
.MJXp-scale0 {-webkit-transform: scaleX(.0); -moz-transform: scaleX(.0); -ms-transform: scaleX(.0); -o-transform: scaleX(.0); transform: scaleX(.0)}
 
.MJXp-scale1 {-webkit-transform: scaleX(.1); -moz-transform: scaleX(.1); -ms-transform: scaleX(.1); -o-transform: scaleX(.1); transform: scaleX(.1)}
 
.MJXp-scale2 {-webkit-transform: scaleX(.2); -moz-transform: scaleX(.2); -ms-transform: scaleX(.2); -o-transform: scaleX(.2); transform: scaleX(.2)}
 
.MJXp-scale3 {-webkit-transform: scaleX(.3); -moz-transform: scaleX(.3); -ms-transform: scaleX(.3); -o-transform: scaleX(.3); transform: scaleX(.3)}
 
.MJXp-scale4 {-webkit-transform: scaleX(.4); -moz-transform: scaleX(.4); -ms-transform: scaleX(.4); -o-transform: scaleX(.4); transform: scaleX(.4)}
 
.MJXp-scale5 {-webkit-transform: scaleX(.5); -moz-transform: scaleX(.5); -ms-transform: scaleX(.5); -o-transform: scaleX(.5); transform: scaleX(.5)}
 
.MJXp-scale6 {-webkit-transform: scaleX(.6); -moz-transform: scaleX(.6); -ms-transform: scaleX(.6); -o-transform: scaleX(.6); transform: scaleX(.6)}
 
.MJXp-scale7 {-webkit-transform: scaleX(.7); -moz-transform: scaleX(.7); -ms-transform: scaleX(.7); -o-transform: scaleX(.7); transform: scaleX(.7)}
 
.MJXp-scale8 {-webkit-transform: scaleX(.8); -moz-transform: scaleX(.8); -ms-transform: scaleX(.8); -o-transform: scaleX(.8); transform: scaleX(.8)}
 
.MJXp-scale9 {-webkit-transform: scaleX(.9); -moz-transform: scaleX(.9); -ms-transform: scaleX(.9); -o-transform: scaleX(.9); transform: scaleX(.9)}
 
.MathJax_PHTML .noError {vertical-align: ; font-size: 90%; text-align: left; color: black; padding: 1px 3px; border: 1px solid}
 
</style></head>
 
  
<body class="mediawiki  ltr ns-0 ns-subject page-Team_LMU-Munich"><div id="MathJax_Message" style="display: none;"></div>
 
  <div id="globalWrapper">
 
 
      <!-- close top-section-->
 
    <div id="content">
 
<a name="top" id="top" target="_blank"></a>
 
<h1 class="firstHeading">Team:LMU-Munich</h1>
 
<div id="bodyContent">
 
<h3 id="siteSub" class="noprint">From 2014.igem.org</h3>
 
<div id="contentSub"></div>
 
<!--
 
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
 
<!-- start content -->
 
<p>
 
 
<style type="text/css">
 
 
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
 
 
.firstHeading {
 
width: 975px;
 
margin: 0px auto;
 
padding-top: 100px;
 
margin-bottom: 20px;
 
font-family: Georgia, Times, "Times New Roman", serif;
 
}
 
 
h1, h2, h3, h4, h5 { font-family: Georgia, Times, "Times New Roman", serif;}
 
 
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
 
background-color: #383838;
 
border: 0 none;
 
height: 14px;
 
z-index: 100;
 
top: 0;
 
position: fixed;
 
width: 975px;
 
left: 50%;
 
margin-left: -487px;
 
}
 
 
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
 
background-color: #383838;
 
height: 14px;
 
display: block;
 
z-index: 10;
 
position: fixed;
 
width: 100%;
 
top: 0;
 
}
 
 
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
 
color: #727272;
 
text-decoration: none;
 
background-color: transparent;
 
}
 
 
body {
 
background-color: #fff;
 
}
 
 
#globalWrapper, #content { /*-- changes default wiki settings --*/
 
width: 100%;
 
height: 100%;
 
border: 0px;
 
background-color: transparent;
 
margin: 0px;
 
padding: 0px;
 
}
 
 
html, body, .wrapper { /*-- changes default wiki settings --*/
 
width: 100%;
 
height: 100%;
 
background-color: transparent;
 
}
 
 
#contentcontainer { /*-- creates container for all content on page --*/
 
font-family: Arial, Helvetica, sans-serif;
 
font-weight: normal;
 
font-size: 14px;
 
color: #414141;
 
width: 960px;
 
margin-left: auto;
 
margin-right: auto;
 
background-color: #transparent;
 
margin-top: 0px;
 
}
 
 
.sidemenu, .sidemenu li {
 
list-style-type: none;
 
list-style-image: none;
 
font-family: verdana;
 
text-decoration: none;
 
color:#000;
 
font-size: 14px;
 
}
 
 
.sidemenu li {display: block;}
 
 
.sidemenu a {
 
text-decoration:none;
 
color: #000;
 
display: block;
 
width: 180px;
 
height: 100%;
 
padding: 3px 5px;
 
transition: .25s ease-in-out;
 
-moz-transition: .25s ease-in-out;
 
-webkit-transition: .25s ease-in-out;
 
}
 
 
.sidemenu a.greyout { color: #909090; }
 
 
.sidemenu a:hover {
 
color:#666;
 
background-color: #d9f5aa;
 
}
 
 
.sub-sidemenu {font-size: 10px; margin-bottom: 20px; color: #666; display: none;}
 
.sub-sidemenu a {color: #666;}
 
 
div.underconst
 
{
 
padding:10px;
 
border-radius:5px;
 
background-color:pink;
 
text-align:center;
 
}
 
 
a.anchor{display: block; position: relative; top: -80px; visibility: hidden;}
 
 
.contentpara {margin-bottom: 30px;}
 
 
span.email{font-family: monospace; font-weight: normal;}
 
 
fieldset {border: 1px solid #337f53;}
 
 
.greyout {color: #A0A0A0;}
 
.highlightme {background-color: #FFFF00;}
 
 
#alertContainer { margin-bottom: 10px; width: 936px;}
 
#annContainer {margin-left: 8px;}
 
#newsContainer {border: 1px solid #ccc;}
 
 
.newsTitle {
 
display: block;
 
color: #414141;
 
font-size: 25px; 
 
        font-family: Georgia, Times, "Times New Roman", serif;
 
padding: 10px 15px 5px 10px;
 
border-bottom: 1px solid #ccc;
 
margin-bottom: 0px;
 
}
 
 
.newsItem {
 
border-bottom: 1px solid #ccc;
 
display: block;
 
padding: 5px 15px 0px 10px;
 
margin-bottom: 0px;
 
}
 
 
.newsItem h3 {
 
width: auto;
 
display: inline;
 
font-size: 14px;
 
font-family: Arial, Helvetica, sans-serif;
 
padding: 0px;
 
margin: 0px;
 
}
 
 
.newsItem img {
 
float: right;
 
clear: right;
 
width: 80px;
 
padding-left: 15px;
 
display: inline-block;
 
}
 
 
.newsItem .newsDate {
 
font-style: italic;
 
font-size: 14px;
 
display: inline-block;
 
color: #999;
 
float: right;
 
padding: 0px 0px 10px 15px;
 
}
 
 
.newsItem p, .newsItem ul, .newsItem li {
 
padding: 0px;
 
margin-left: 20px;
 
font-size: 12px;
 
line-height: 1.2;
 
}
 
 
.newsItem p {margin-top: 10px;}
 
 
.annItem {
 
border: 2px solid #414141;
 
display: block;
 
padding: 0px 15px 10px 10px;
 
margin-bottom: 18px;
 
position: relative;
 
height: 160px;
 
vertical-align: middle;
 
}
 
 
.annItem .annCentered {
 
display: table-cell;
 
vertical-align: middle;
 
height: 160px;
 
}
 
 
.annCentered h3 {
 
padding: 0px;
 
display: block;
 
margin-left: auto;
 
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<h1> <span class="mw-headline" id="Abstract">Abstract</span></h1>
 
<p>Increasing bacterial resistance to classical antibiotics remains a serious threat and urges the development of novel pathogen-killing strategies. Exploiting bacterial communication mechanisms such as quorum sensing is a promising strategy to specifically target certain pathogens. The major aim of this project is the introduction of a genetic circuit enabling <i>Bacillus subtilis</i> to actively detect, attach to, and eventually kill <i>Staphylococcus aureus</i> and <i>Streptococcus pneumoniae</i>.
 
</p><p>Initially, we will introduce the autoinducer-sensing two-component systems of <i>S. aureus</i> and <i>S. pneumoniae</i> into <i>B. subtilis</i>.  to create a pathogen-detecting strain. By utilizing quorum sensing-dependent promoters, we will then trigger pathogen-killing strategies like the production of antimicrobial peptides or biofilm degradation. As a safety measure a delayed suicide-switch guarantees non-persistence of genetically modified <i>B. subtilis</i> in the absence of pathogens. We envision the use of BaKillus as a smart, cheap and simple-to-use medical device for diagnostics and targeted treatment of multiresistant super bugs.
 
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<h2> <span class="mw-headline" id="Hi_there.21"> Hi there! </span></h2>
 
<p>Welcome to our Wiki! I'm BaKillus, the pathogen-hunting microbe, and I'll guide you on this tour through our project. If you want to learn more about a specific step, you can simply close the tour and come back to it anytime you like. So let's start!
 
 
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<h2> <span class="mw-headline" id="What.27s_the_problem.3F"> What's the problem? </span></h2>
 
<p>First of all, what am I doing here? The problem is, pathogenic bacteria all around the world are becoming more and more  resistant against antimicrobial drugs. One major reason for the trend is the inappropriate use of drugs. With my BaKillus super powers, I want to reduce this misuse and thus do my part to save global health.
 
 
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<p></p>
 
<h2> <span class="mw-headline" id="Sensing_of_pathogens"> Sensing of pathogens </span></h2>
 
<p>To combat the pathogenic bacteria, I simply eavesdrop on their communication. Bacteria talk with each other via quorum sensing systems, which I use to detect them and trigger my responses.
 
 
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<h2> <span class="mw-headline" id="Adhesion"> Adhesion </span></h2>
 
<p>The more specific and effective I can use my powers, the lower the danger is of provoking new resistance development. So I catch pathogens whenever I get hold of them and stick to them until my work is done.
 
 
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<h2> <span class="mw-headline" id="Killing"> Killing </span></h2>
 
<p>Talking about my work - killing pathogens is finally what I am made for. In response to quorum sensing molecules of the pathogens, I export a range of antimicrobial substances leading to dissipation of biofilms and the killing of the targeted bacteria.
 
 
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<h2> <span class="mw-headline" id="Suicide_switch"> Suicide switch </span></h2>
 
<p>When the job is done and all the bad guys are finished, you don't need a super hero anymore. So after fulfilling my work I say goodbye to the world by activating my suicide switch.
 
 
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<h2> <span class="mw-headline" id="Application"> Application </span></h2>
 
<p>Of course I'm not only a fictional hero, but a very real one. In two different prototypes, I could be used for diagnosis or treatment of pathogen-caused diseases. However, there is still a whole lot of regulational and economical questions that have to be answered before.
 
 
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<h2> <span class="mw-headline" id="See_you.21"> See you! </span></h2>
 
<p>So now you know my short story - and it is time for me to return to my fight for a safer world. Feel free to take a closer look on my <a href="http://2014.igem.org/Team:LMU-Munich/Project/Bakillus" class="external text" rel="nofollow">super powers</a>, the <a href="http://2014.igem.org/Team:LMU-Munich/Notebook/Timeline" class="external text" rel="nofollow">process of my development</a> or the plans for a <a href="http://2014.igem.org/Team:LMU-Munich/Application" class="external text" rel="nofollow">medical application</a>.
 
 
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Revision as of 23:59, 28 September 2017