Difference between revisions of "Template:Example"

(Prototype team template page)
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−
<html>
+
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"><head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
<meta name="generator" content="MediaWiki 1.16.5">
 +
<link rel="shortcut icon" href="/favicon.ico">
 +
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2014.igem.org (en)">
 +
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:LMU-Munich&amp;action=creativecommons" rel="meta">
 +
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/">
 +
<link rel="alternate" type="application/atom+xml" title="2014.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom"> <title>Team:LMU-Munich - 2014.igem.org</title>
 +
<style type="text/css" media="screen, projection">/*<![CDATA[*/
 +
@import "/wiki/skins/common/shared.css?270";
 +
@import "/wiki/skins/igem/main.css?270";
 +
/*]]>*/</style>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270">
 +
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
 +
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
 +
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
 +
 +
<script type="text/javascript" async="" src="http://www.google-analytics.com/plugins/ua/linkid.js"></script><script async="" src="//www.google-analytics.com/analytics.js"></script><script>
 +
var skin="igem",
 +
stylepath="/wiki/skins",
 +
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 +
wgArticlePath="/$1",
 +
wgScriptPath="/wiki",
 +
wgScriptExtension=".php",
 +
wgScript="/wiki/index.php",
 +
wgVariantArticlePath=false,
 +
wgActionPaths={},
 +
wgServer="https://2014.igem.org",
 +
wgCanonicalNamespace="",
 +
wgCanonicalSpecialPageName=false,
 +
wgNamespaceNumber=0,
 +
wgPageName="Team:LMU-Munich",
 +
wgTitle="Team:LMU-Munich",
 +
wgAction="view",
 +
wgArticleId=1189,
 +
wgIsArticle=true,
 +
wgUserName=null,
 +
wgUserGroups=null,
 +
wgUserLanguage="en",
 +
wgContentLanguage="en",
 +
wgBreakFrames=false,
 +
wgCurRevisionId=403769,
 +
wgVersion="1.16.5",
 +
wgEnableAPI=true,
 +
wgEnableWriteAPI=true,
 +
wgSeparatorTransformTable=["", ""],
 +
wgDigitTransformTable=["", ""],
 +
wgMainPageTitle="Main Page",
 +
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2014.igem.org", "5": "2014.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 +
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2014.igem.org": 4, "2014.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 +
wgSiteName="2014.igem.org",
 +
wgCategories=[],
 +
wgMWSuggestTemplate="https://2014.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 +
wgDBname="2014_igem_org",
 +
wgSearchNamespaces=[0],
 +
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 +
wgRestrictionEdit=[],
 +
wgRestrictionMove=[];
 +
</script>               
 +
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
 +
<script src="/wiki/skins/common/ajax.js?270"></script>
 +
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 +
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
<!-- jQuery Javascript -->
 +
<script type="text/javascript" src="/common/jquery-latest.min.js"></script>
 +
<script type="text/javascript" src="/common/tablesorter/jquery.tablesorter.min.js"></script>
 +
        <link rel="stylesheet" type="text/css" href="/common/tablesorter/themes/groupparts/style.css">
 +
        <link rel="stylesheet" type="text/css" href="/common/table_styles.css">
 +
        <link rel="stylesheet" type="text/css" href="/forum/forum_styles.css">
 +
<script type="text/javascript" src="/forum/forum_scripts.js"></script>
 +
<style type="text/css">.MathJax_Hover_Frame {border-radius: .25em; -webkit-border-radius: .25em; -moz-border-radius: .25em; -khtml-border-radius: .25em; box-shadow: 0px 0px 15px #83A; -webkit-box-shadow: 0px 0px 15px #83A; -moz-box-shadow: 0px 0px 15px #83A; -khtml-box-shadow: 0px 0px 15px #83A; border: 1px solid #A6D ! important; display: inline-block; position: absolute}
 +
.MathJax_Menu_Button .MathJax_Hover_Arrow {position: absolute; cursor: pointer; display: inline-block; border: 2px solid #AAA; border-radius: 4px; -webkit-border-radius: 4px; -moz-border-radius: 4px; -khtml-border-radius: 4px; font-family: 'Courier New',Courier; font-size: 9px; color: #F0F0F0}
 +
.MathJax_Menu_Button .MathJax_Hover_Arrow span {display: block; background-color: #AAA; border: 1px solid; border-radius: 3px; line-height: 0; padding: 4px}
 +
.MathJax_Hover_Arrow:hover {color: white!important; border: 2px solid #CCC!important}
 +
.MathJax_Hover_Arrow:hover span {background-color: #CCC!important}
 +
</style><style type="text/css">#MathJax_About {position: fixed; left: 50%; width: auto; text-align: center; border: 3px outset; padding: 1em 2em; background-color: #DDDDDD; color: black; cursor: default; font-family: message-box; font-size: 120%; font-style: normal; text-indent: 0; text-transform: none; line-height: normal; letter-spacing: normal; word-spacing: normal; word-wrap: normal; white-space: nowrap; float: none; z-index: 201; border-radius: 15px; -webkit-border-radius: 15px; -moz-border-radius: 15px; -khtml-border-radius: 15px; box-shadow: 0px 10px 20px #808080; -webkit-box-shadow: 0px 10px 20px #808080; -moz-box-shadow: 0px 10px 20px #808080; -khtml-box-shadow: 0px 10px 20px #808080; filter: progid:DXImageTransform.Microsoft.dropshadow(OffX=2, OffY=2, Color='gray', Positive='true')}
 +
#MathJax_About.MathJax_MousePost {outline: none}
 +
.MathJax_Menu {position: absolute; background-color: white; color: black; width: auto; padding: 2px; border: 1px solid #CCCCCC; margin: 0; cursor: default; font: menu; text-align: left; text-indent: 0; text-transform: none; line-height: normal; letter-spacing: normal; word-spacing: normal; word-wrap: normal; white-space: nowrap; float: none; z-index: 201; box-shadow: 0px 10px 20px #808080; -webkit-box-shadow: 0px 10px 20px #808080; -moz-box-shadow: 0px 10px 20px #808080; -khtml-box-shadow: 0px 10px 20px #808080; filter: progid:DXImageTransform.Microsoft.dropshadow(OffX=2, OffY=2, Color='gray', Positive='true')}
 +
.MathJax_MenuItem {padding: 2px 2em; background: transparent}
 +
.MathJax_MenuArrow {position: absolute; right: .5em; padding-top: .25em; color: #666666; font-size: .75em}
 +
.MathJax_MenuActive .MathJax_MenuArrow {color: white}
 +
.MathJax_MenuArrow.RTL {left: .5em; right: auto}
 +
.MathJax_MenuCheck {position: absolute; left: .7em}
 +
.MathJax_MenuCheck.RTL {right: .7em; left: auto}
 +
.MathJax_MenuRadioCheck {position: absolute; left: 1em}
 +
.MathJax_MenuRadioCheck.RTL {right: 1em; left: auto}
 +
.MathJax_MenuLabel {padding: 2px 2em 4px 1.33em; font-style: italic}
 +
.MathJax_MenuRule {border-top: 1px solid #CCCCCC; margin: 4px 1px 0px}
 +
.MathJax_MenuDisabled {color: GrayText}
 +
.MathJax_MenuActive {background-color: Highlight; color: HighlightText}
 +
.MathJax_MenuDisabled:focus, .MathJax_MenuLabel:focus {background-color: #E8E8E8}
 +
.MathJax_ContextMenu:focus {outline: none}
 +
.MathJax_ContextMenu .MathJax_MenuItem:focus {outline: none}
 +
#MathJax_AboutClose {top: .2em; right: .2em}
 +
.MathJax_Menu .MathJax_MenuClose {top: -10px; left: -10px}
 +
.MathJax_MenuClose {position: absolute; cursor: pointer; display: inline-block; border: 2px solid #AAA; border-radius: 18px; -webkit-border-radius: 18px; -moz-border-radius: 18px; -khtml-border-radius: 18px; font-family: 'Courier New',Courier; font-size: 24px; color: #F0F0F0}
 +
.MathJax_MenuClose span {display: block; background-color: #AAA; border: 1.5px solid; border-radius: 18px; -webkit-border-radius: 18px; -moz-border-radius: 18px; -khtml-border-radius: 18px; line-height: 0; padding: 8px 0 6px}
 +
.MathJax_MenuClose:hover {color: white!important; border: 2px solid #CCC!important}
 +
.MathJax_MenuClose:hover span {background-color: #CCC!important}
 +
.MathJax_MenuClose:hover:focus {outline: none}
 +
</style><style type="text/css">.MathJax_Preview .MJXf-math {color: inherit!important}
 +
</style><style type="text/css">.MJX_Assistive_MathML {position: absolute!important; top: 0; left: 0; clip: rect(1px, 1px, 1px, 1px); padding: 1px 0 0 0!important; border: 0!important; height: 1px!important; width: 1px!important; overflow: hidden!important; display: block!important; -webkit-touch-callout: none; -webkit-user-select: none; -khtml-user-select: none; -moz-user-select: none; -ms-user-select: none; user-select: none}
 +
.MJX_Assistive_MathML.MJX_Assistive_MathML_Block {width: 100%!important}
 +
</style><style type="text/css">#MathJax_Zoom {position: absolute; background-color: #F0F0F0; overflow: auto; display: block; z-index: 301; padding: .5em; border: 1px solid black; margin: 0; font-weight: normal; font-style: normal; text-align: left; text-indent: 0; text-transform: none; line-height: normal; letter-spacing: normal; word-spacing: normal; word-wrap: normal; white-space: nowrap; float: none; -webkit-box-sizing: content-box; -moz-box-sizing: content-box; box-sizing: content-box; box-shadow: 5px 5px 15px #AAAAAA; -webkit-box-shadow: 5px 5px 15px #AAAAAA; -moz-box-shadow: 5px 5px 15px #AAAAAA; -khtml-box-shadow: 5px 5px 15px #AAAAAA; filter: progid:DXImageTransform.Microsoft.dropshadow(OffX=2, OffY=2, Color='gray', Positive='true')}
 +
#MathJax_ZoomOverlay {position: absolute; left: 0; top: 0; z-index: 300; display: inline-block; width: 100%; height: 100%; border: 0; padding: 0; margin: 0; background-color: white; opacity: 0; filter: alpha(opacity=0)}
 +
#MathJax_ZoomFrame {position: relative; display: inline-block; height: 0; width: 0}
 +
#MathJax_ZoomEventTrap {position: absolute; left: 0; top: 0; z-index: 302; display: inline-block; border: 0; padding: 0; margin: 0; background-color: white; opacity: 0; filter: alpha(opacity=0)}
 +
</style><style type="text/css">.MathJax_Preview {color: #888}
 +
#MathJax_Message {position: fixed; left: 1em; bottom: 1.5em; background-color: #E6E6E6; border: 1px solid #959595; margin: 0px; padding: 2px 8px; z-index: 102; color: black; font-size: 80%; width: auto; white-space: nowrap}
 +
#MathJax_MSIE_Frame {position: absolute; top: 0; left: 0; width: 0px; z-index: 101; border: 0px; margin: 0px; padding: 0px}
 +
.MathJax_Error {color: #CC0000; font-style: italic}
 +
</style><style type="text/css">.MJXp-script {font-size: .8em}
 +
.MJXp-right {-webkit-transform-origin: right; -moz-transform-origin: right; -ms-transform-origin: right; -o-transform-origin: right; transform-origin: right}
 +
.MJXp-bold {font-weight: bold}
 +
.MJXp-italic {font-style: italic}
 +
.MJXp-scr {font-family: MathJax_Script,'Times New Roman',Times,STIXGeneral,serif}
 +
.MJXp-frak {font-family: MathJax_Fraktur,'Times New Roman',Times,STIXGeneral,serif}
 +
.MJXp-sf {font-family: MathJax_SansSerif,'Times New Roman',Times,STIXGeneral,serif}
 +
.MJXp-cal {font-family: MathJax_Caligraphic,'Times New Roman',Times,STIXGeneral,serif}
 +
.MJXp-mono {font-family: MathJax_Typewriter,'Times New Roman',Times,STIXGeneral,serif}
 +
.MJXp-largeop {font-size: 150%}
 +
.MJXp-largeop.MJXp-int {vertical-align: -.2em}
 +
.MJXp-math {display: inline-block; line-height: 1.2; text-indent: 0; font-family: 'Times New Roman',Times,STIXGeneral,serif; white-space: nowrap; border-collapse: collapse}
 +
.MJXp-display {display: block; text-align: center; margin: 1em 0}
 +
.MJXp-math span {display: inline-block}
 +
.MJXp-box {display: block!important; text-align: center}
 +
.MJXp-box:after {content: " "}
 +
.MJXp-rule {display: block!important; margin-top: .1em}
 +
.MJXp-char {display: block!important}
 +
.MJXp-mo {margin: 0 .15em}
 +
.MJXp-mfrac {margin: 0 .125em; vertical-align: .25em}
 +
.MJXp-denom {display: inline-table!important; width: 100%}
 +
.MJXp-denom > * {display: table-row!important}
 +
.MJXp-surd {vertical-align: top}
 +
.MJXp-surd > * {display: block!important}
 +
.MJXp-script-box > *  {display: table!important; height: 50%}
 +
.MJXp-script-box > * > * {display: table-cell!important; vertical-align: top}
 +
.MJXp-script-box > *:last-child > * {vertical-align: bottom}
 +
.MJXp-script-box > * > * > * {display: block!important}
 +
.MJXp-mphantom {visibility: hidden}
 +
.MJXp-munderover {display: inline-table!important}
 +
.MJXp-over {display: inline-block!important; text-align: center}
 +
.MJXp-over > * {display: block!important}
 +
.MJXp-munderover > * {display: table-row!important}
 +
.MJXp-mtable {vertical-align: .25em; margin: 0 .125em}
 +
.MJXp-mtable > * {display: inline-table!important; vertical-align: middle}
 +
.MJXp-mtr {display: table-row!important}
 +
.MJXp-mtd {display: table-cell!important; text-align: center; padding: .5em 0 0 .5em}
 +
.MJXp-mtr > .MJXp-mtd:first-child {padding-left: 0}
 +
.MJXp-mtr:first-child > .MJXp-mtd {padding-top: 0}
 +
.MJXp-mlabeledtr {display: table-row!important}
 +
.MJXp-mlabeledtr > .MJXp-mtd:first-child {padding-left: 0}
 +
.MJXp-mlabeledtr:first-child > .MJXp-mtd {padding-top: 0}
 +
.MJXp-merror {background-color: #FFFF88; color: #CC0000; border: 1px solid #CC0000; padding: 1px 3px; font-style: normal; font-size: 90%}
 +
.MJXp-scale0 {-webkit-transform: scaleX(.0); -moz-transform: scaleX(.0); -ms-transform: scaleX(.0); -o-transform: scaleX(.0); transform: scaleX(.0)}
 +
.MJXp-scale1 {-webkit-transform: scaleX(.1); -moz-transform: scaleX(.1); -ms-transform: scaleX(.1); -o-transform: scaleX(.1); transform: scaleX(.1)}
 +
.MJXp-scale2 {-webkit-transform: scaleX(.2); -moz-transform: scaleX(.2); -ms-transform: scaleX(.2); -o-transform: scaleX(.2); transform: scaleX(.2)}
 +
.MJXp-scale3 {-webkit-transform: scaleX(.3); -moz-transform: scaleX(.3); -ms-transform: scaleX(.3); -o-transform: scaleX(.3); transform: scaleX(.3)}
 +
.MJXp-scale4 {-webkit-transform: scaleX(.4); -moz-transform: scaleX(.4); -ms-transform: scaleX(.4); -o-transform: scaleX(.4); transform: scaleX(.4)}
 +
.MJXp-scale5 {-webkit-transform: scaleX(.5); -moz-transform: scaleX(.5); -ms-transform: scaleX(.5); -o-transform: scaleX(.5); transform: scaleX(.5)}
 +
.MJXp-scale6 {-webkit-transform: scaleX(.6); -moz-transform: scaleX(.6); -ms-transform: scaleX(.6); -o-transform: scaleX(.6); transform: scaleX(.6)}
 +
.MJXp-scale7 {-webkit-transform: scaleX(.7); -moz-transform: scaleX(.7); -ms-transform: scaleX(.7); -o-transform: scaleX(.7); transform: scaleX(.7)}
 +
.MJXp-scale8 {-webkit-transform: scaleX(.8); -moz-transform: scaleX(.8); -ms-transform: scaleX(.8); -o-transform: scaleX(.8); transform: scaleX(.8)}
 +
.MJXp-scale9 {-webkit-transform: scaleX(.9); -moz-transform: scaleX(.9); -ms-transform: scaleX(.9); -o-transform: scaleX(.9); transform: scaleX(.9)}
 +
.MathJax_PHTML .noError {vertical-align: ; font-size: 90%; text-align: left; color: black; padding: 1px 3px; border: 1px solid}
 +
</style></head>
  
−
<script>
+
<body class="mediawiki  ltr ns-0 ns-subject page-Team_LMU-Munich"><div id="MathJax_Message" style="display: none;"></div>
 +
  <div id="globalWrapper">
  
−
// This is the jquery part of your template.
+
      <!-- close top-section-->
−
// Try not modify any of this code too much since it makes your menu work.
+
    <div id="content">
 +
<a name="top" id="top" target="_blank"></a>
 +
<h1 class="firstHeading">Team:LMU-Munich</h1>
 +
<div id="bodyContent">
 +
<h3 id="siteSub" class="noprint">From 2014.igem.org</h3>
 +
<div id="contentSub"></div>
 +
<!--
 +
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
 +
<!-- start content -->
 +
<p>
  
−
$(document).ready(function() {
+
<style type="text/css">
  
−
$("#HQ_page").attr('id','');
+
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
  
−
// call the functions that control the menu
+
.firstHeading {
−
menu_functionality();  
+
width: 975px;  
−
hide_show_menu();
+
margin: 0px auto;
 +
padding-top: 100px;
 +
margin-bottom: 20px;
 +
font-family: Georgia, Times, "Times New Roman", serif;
 +
}
  
 +
h1, h2, h3, h4, h5 { font-family: Georgia, Times, "Times New Roman", serif;}
  
 +
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
 +
background-color: #383838;
 +
border: 0 none;
 +
height: 14px;
 +
z-index: 100;
 +
top: 0;
 +
position: fixed;
 +
width: 975px;
 +
left: 50%;
 +
margin-left: -487px;
 +
}
  
−
//this function controls the expand and collapse buttons of the menu and changes the +/- symbols
+
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
−
function menu_functionality() {
+
background-color: #383838;
 +
height: 14px;
 +
display: block;
 +
z-index: 10;
 +
position: fixed;
 +
width: 100%;
 +
top: 0;
 +
}
  
−
//when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
+
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
−
$(".menu_button").click(function(){
+
color: #727272;
 +
text-decoration: none;
 +
background-color: transparent;
 +
}
  
−
// add or remove the class "open" , this class holds the "-"
+
body {
−
$(this).children().toggleClass("open");
+
background-color: #fff;
−
// show or hide the submenu
+
}
−
$(this).next('.submenu_wrapper').fadeToggle(400);
+
−
});
+
  
−
// when the screen size is smaller than 800px, the display_menu_control button appears and will show/hide the whole menu
+
#globalWrapper, #content { /*-- changes default wiki settings --*/
−
$("#display_menu_control").click(function(){
+
width: 100%;  
−
$('#menu_content').fadeToggle(400);
+
height: 100%;
−
});
+
border: 0px;
 +
background-color: transparent;
 +
margin: 0px;
 +
padding: 0px;
 +
}
  
−
// call the current page highlight function
+
html, body, .wrapper { /*-- changes default wiki settings --*/
−
highlight_current_page();
+
width: 100%;  
−
}
+
height: 100%;
 +
background-color: transparent;
 +
}
  
 +
#contentcontainer { /*-- creates container for all content on page --*/
 +
font-family: Arial, Helvetica, sans-serif;
 +
font-weight: normal;
 +
font-size: 14px;
 +
color: #414141;
 +
width: 960px;
 +
margin-left: auto;
 +
margin-right: auto;
 +
background-color: #transparent;
 +
margin-top: 0px;
 +
}
  
−
// call the highlight current page function to show it on the menu with a different background color
+
.sidemenu, .sidemenu li {
−
function highlight_current_page() {
+
list-style-type: none;
 +
list-style-image: none;
 +
font-family: verdana;
 +
text-decoration: none;
 +
color:#000;
 +
font-size: 14px;
 +
}
  
−
// select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
+
.sidemenu li {display: block;}
−
$("#"+  wgPageName.substring(wgPageName.lastIndexOf("/")+1, wgPageName.length ) + "_page").addClass("current_page");
+
  
−
// now that the current_page class has been added to a menu item, make the submenu fade in
+
.sidemenu a {
−
$(".current_page").parents(".submenu_wrapper").fadeIn(400);
+
text-decoration:none;  
−
// change the +/- symbol of the corresponding menu button
+
color: #000;
−
$(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
+
display: block;
−
+
width: 180px;
−
}
+
height: 100%;
 +
padding: 3px 5px;
 +
transition: .25s ease-in-out;
 +
-moz-transition: .25s ease-in-out;
 +
-webkit-transition: .25s ease-in-out;
 +
}  
  
 +
.sidemenu a.greyout { color: #909090; }
  
 +
.sidemenu a:hover {
 +
color:#666;
 +
background-color: #d9f5aa;
 +
}
  
−
// allow button on the black menu bar to show/hide the side menu
+
.sub-sidemenu {font-size: 10px; margin-bottom: 20px; color: #666; display: none;}
−
function hide_show_menu() {
+
.sub-sidemenu a {color: #666;}
−
+
−
// in case you preview mode is selected, the menu is hidden for better visibility
+
−
if (window.location.href.indexOf("submit") >= 0) {
+
−
$(".igem_2017_menu_wrapper").hide();
+
−
}
+
  
−
// if the black menu bar has been loaded
+
div.underconst
−
  if (document.getElementById('bars_item')) {
+
{
 +
padding:10px;
 +
border-radius:5px;
 +
background-color:pink;
 +
text-align:center;
 +
}
  
−
// when the "bars_item" has been clicked
+
a.anchor{display: block; position: relative; top: -80px; visibility: hidden;}
−
$("#bars_item").click(function() {
+
−
$("#sideMenu").hide();
+
  
−
// show/hide the menu wrapper
+
.contentpara {margin-bottom: 30px;}
−
$(".igem_2017_menu_wrapper").fadeToggle("100");
+
−
});
+
−
  }  
+
  
−
// because the black menu bars loads at a different time than the rest of the page, this function is set on a time out so it can run again in case it has not been loaded yet
+
span.email{font-family: monospace; font-weight: normal;}
−
else {
+
−
    setTimeout(hide_show_menu, 15);
+
−
}
+
−
}
+
  
 +
fieldset {border: 1px solid #337f53;}
  
−
});
+
.greyout {color: #A0A0A0;}
 +
.highlightme {background-color: #FFFF00;}
  
 +
#alertContainer { margin-bottom: 10px; width: 936px;}
 +
#annContainer {margin-left: 8px;}
 +
#newsContainer {border: 1px solid #ccc;}
  
−
</script>
+
.newsTitle {
 +
display: block;
 +
color: #414141;
 +
font-size: 25px; 
 +
        font-family: Georgia, Times, "Times New Roman", serif;
 +
padding: 10px 15px 5px 10px;
 +
border-bottom: 1px solid #ccc;
 +
margin-bottom: 0px;
 +
}
  
 +
.newsItem {
 +
border-bottom: 1px solid #ccc;
 +
display: block;
 +
padding: 5px 15px 0px 10px;
 +
margin-bottom: 0px;
 +
}
  
 +
.newsItem h3 {
 +
width: auto;
 +
display: inline;
 +
font-size: 14px;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding: 0px;
 +
margin: 0px;
 +
}
  
 +
.newsItem img {
 +
float: right;
 +
clear: right;
 +
width: 80px;
 +
padding-left: 15px;
 +
display: inline-block;
 +
}
  
 +
.newsItem .newsDate {
 +
font-style: italic;
 +
font-size: 14px;
 +
display: inline-block;
 +
color: #999;
 +
float: right;
 +
padding: 0px 0px 10px 15px;
 +
}
  
−
<style>
+
.newsItem p, .newsItem ul, .newsItem li {
 +
padding: 0px;
 +
margin-left: 20px;
 +
font-size: 12px;
 +
line-height: 1.2;
 +
}
  
 +
.newsItem p {margin-top: 10px;}
  
 +
.annItem {
 +
border: 2px solid #414141;
 +
display: block;
 +
padding: 0px 15px 10px 10px;
 +
margin-bottom: 18px;
 +
position: relative;
 +
height: 160px;
 +
vertical-align: middle;
 +
}
  
−
/***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
+
.annItem .annCentered {
−
+
display: table-cell;  
−
/* Clear the default wiki settings */
+
vertical-align: middle;  
−
+
height: 160px;
−
#home_logo, #sideMenu { display:none; }
+
}
−
#sideMenu, #top_title, .patrollink  {display:none;}
+
−
#content { width:100%; padding:0px;  margin-top:-7px; margin-left:0px;}
+
−
body {background-color:white; }
+
−
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
  
−
+
.annCentered h3 {
−
+
padding: 0px;
−
+
display: block;  
−
/**************************************************************** MENU ***************************************************************/
+
margin-left: auto;  
−
/* Wrapper for the menu */
+
margin-right: auto;  
−
.igem_2017_menu_wrapper {  
+
text-align: center;
−
width: 15%;
+
}
−
height:100vh;
+
−
position:fixed;
+
−
right:0%;
+
−
padding:0px;
+
−
float:right;  
+
−
border-left: 1px solid #d3d3d3;  
+
−
background-color:#dddddd;  
+
−
text-align:left;
+
−
font-family:Tahoma, Geneva, sans-serif;
+
−
overflow-y: auto;
+
−
overflow-x: hidden;
+
−
}
+
  
−
/* this hides the scrollbar to keep view consistency */
+
.annItem ul, .annItem li {padding: 0px; margin: 0px;}
−
.igem_2017_menu_wrappe::-webkit-scrollbar {  
+
.annItem li {list-style: none; margin: 5px}
−
display: none;  
+
−
}
+
−
+
−
+
−
/* styling for links in the menu, removes the line under text */
+
−
.igem_2017_menu_wrapper a {
+
−
text-decoration: none;  
+
−
}
+
  
  
−
/* styling for the images in the menu */
 
−
.igem_2017_menu_wrapper img {
 
−
width: 100%;
 
−
}
 
  
−
/* styling for the menu buttons */
+
</style>
−
.igem_2017_menu_wrapper .menu_button {
+
−
width: 100%;
+
−
padding: 10px 0px 10px 15px;
+
−
float:left;
+
−
border-bottom: 1px solid #d3d3d3;
+
−
font-size: 12px;
+
−
font-weight: bold;
+
−
color: #5e5f5f;
+
−
cursor: pointer;
+
−
}
+
  
  
−
.igem_2017_menu_wrapper .menu_bottom_padding {
 
−
width: 100%;
 
−
height: 30px;
 
−
float:left;
 
−
}
 
  
−
.menu_button.direct_to_page {
 
−
padding-left: 36px;
 
−
}
 
  
  
−
.igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
 
−
width:10%;
 
−
float:left;
 
−
}
 
  
−
.igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
 
−
content: "+"; 
 
−
}
 
−
 
−
.open::before {
 
−
content: "-" !important; 
 
−
}
 
−
 
−
 
−
 
−
/* styling for the menu buttons on hover */
 
−
.igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover ,  .submenu_button.current_page:hover {
 
−
background-color: #3399ff;
 
−
text-decoration: none;
 
−
color:#ffffff;
 
−
}
 
  
−
/* styling for the menu button when it is the current page */
+
<!-- NEW JQUERY -->
−
.current_page {
+
<script src="https://code.jquery.com/jquery-1.11.1.min.js"></script>
−
background-color:#7fc1f7  !important;
+
−
color:#5e5f5f !important;
+
−
}
+
  
 +
<!-- MathJax (LaTeX for the web) -->
 +
<script src="https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.1/MathJax.js?config=TeX-AMS-MML_HTMLorMML" id=""></script>
  
−
/* styling for the submenu buttons */
+
<script type="text/javascript" src="http://cdnjs.cloudflare.com/ajax/libs/underscore.js/1.7.0/underscore-min.js"></script>
−
.igem_2017_menu_wrapper .submenu_button {
+
−
width: 100%;
+
−
padding: 10px 0px 10px 34px;
+
−
float:left;
+
−
background-color:#f2f2f2;
+
−
border-bottom: 1px solid #d3d3d3;
+
−
font-size: 12px;
+
−
color: #5e5f5f;
+
−
cursor: pointer;
+
−
}
+
  
−
/* wrapper for the submenu items, they are hidden by default*/
+
<!-- BOOTSTRAP -->
−
.igem_2017_menu_wrapper .submenu_wrapper {
+
<!-- Latest compiled and minified CSS -->
−
display:none;
+
<link href="//maxcdn.bootstrapcdn.com/bootswatch/3.2.0/sandstone/bootstrap.min.css" rel="stylesheet">
−
}
+
<!-- Latest compiled and minified JavaScript -->
 +
<script src="https://2014.igem.org/Team:LMU-Munich/bootstrap.min.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/xscrolly.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/native.history.min.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/ajaxload.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/highcharts.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/highcharts-more.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/LMU_bxslider.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/LMU_slimbox2.js?action=raw&amp;ctype=text/javascript"></script>
 +
<link href="https://2014.igem.org/Team:LMU-Munich/slimbox2.css?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
 +
<link href="https://2014.igem.org/Team:LMU-Munich/bxslider.css?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
 +
<script src="https://2014.igem.org/Team:LMU-Munich/jquery.hoverIntent.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/jquery.scrollTo.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/jquery.tablesorter.js?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2014.igem.org/Team:LMU-Munich/jquery.slicknav.js?action=raw&amp;ctype=text/javascript"></script>
 +
<link href="https://2014.igem.org/Team:LMU-Munich/slicknav.css?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
 +
<script src="https://2014.igem.org/Team:LMU-Munich/iGEM.js?action=raw&amp;ctype=text/javascript"></script>
  
−
/* when the page size is bigger than 800px, this show/hide control is hidden by default */
+
<!-- TOUR STUFF -->
−
.igem_2017_menu_wrapper #display_menu_control {
+
−
display:none;
+
−
text-align:center;
+
−
}
+
−
+
  
−
/***************************************************** CONTENT OF THE PAGE ****************************************************/
+
<script src="https://2014.igem.org/Team:LMU-Munich/iGEM.tour.js?action=raw&amp;ctype=text/javascript"></script>
 +
<link href="https://2014.igem.org/Team:LMU-Munich/iGEM.tour.css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
<!--
 +
<script src="https://preview.c9.io/loxos/igemlmu/offlineVersion/igem-tour.js"></script>
 +
<link href="https://preview.c9.io/loxos/igemlmu/offlineVersion/tour.css" rel="stylesheet">
 +
-->
  
−
/* Wrapper for the content */
+
<!-- MAIN STYLE -->
−
.igem_2017_content_wrapper {
+
<link href="https://2014.igem.org/Team:LMU-Munich/iGEM.css?action=raw&amp;ctype=text/css" rel="stylesheet">
−
width: 81%;
+
−
margin: 2%;
+
−
display:block;
+
−
float:left;
+
−
background-color:white;  
+
−
font-family:Tahoma, Geneva, sans-serif;
+
−
}
+
  
 +
<script>
 +
  (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
 +
  (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
 +
  m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
 +
  })(window,document,'script','//www.google-analytics.com/analytics.js','ga');
  
−
/********************************* HTML STYLING  *********************************/
+
  ga('create', 'UA-55868873-1', 'auto');
 +
  ga('require', 'displayfeatures');
 +
  ga('require', 'linkid', 'linkid.js');
 +
  ga('send', 'pageview');
  
−
/* styling for the titles h1 h2 */
+
</script>
−
.igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
+
−
padding:5px 15px;
+
−
border-bottom: 0px;
+
−
color: #3399ff;
+
−
}
+
  
  
−
/* styling for the titles  h3 h4 h5 h6*/
 
−
.igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
 
−
padding:5px 15px;
 
−
border-bottom:0px;
 
−
color: #000000; 
 
−
}
 
  
 +
</p><p>
 +
</p><div id="slick-navigation"><div class="slicknav_menu hidden-md hidden-lg"><a href="#" aria-haspopup="true" tabindex="0" class="slicknav_btn slicknav_open" style="outline: none;"><span class="slicknav_menutxt">LMU iGEM</span><span class="slicknav_icon"><span class="slicknav_icon-bar"></span><span class="slicknav_icon-bar"></span><span class="slicknav_icon-bar"></span></span></a><div class="slicknav_nav" style="display: block;" aria-hidden="false" role="menu">
 +
        <ul>
 +
            <li class="slicknav_collapsed slicknav_parent"><a href="#" role="menuitem" aria-haspopup="true" tabindex="-1" class="slicknav_item slicknav_row" style="outline: none;">
 +
                <a href="https://2014.igem.org/Team:LMU-Munich/Team">Team</a>
 +
                <span class="slicknav_arrow">►</span></a><ul role="menu" class="slicknav_hidden" style="display: none;" aria-hidden="true">
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Team" role="menuitem" tabindex="-1">Team</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Team/Financing" role="menuitem" tabindex="-1">Financing</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Team/Collaborations" role="menuitem" tabindex="-1">Collaborations</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Team/Attributions" role="menuitem" tabindex="-1">Attributions</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="slicknav_collapsed slicknav_parent"><a href="#" role="menuitem" aria-haspopup="true" tabindex="-1" class="slicknav_item slicknav_row" style="outline: none;">
 +
                <a href="https://2014.igem.org/Team:LMU-Munich/Project">Project</a>
 +
                <span class="slicknav_arrow">►</span></a><ul role="menu" class="slicknav_hidden" style="display: none;" aria-hidden="true">
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/Problem" role="menuitem" tabindex="-1">Problem</a></li>
 +
                    <li class="slicknav_collapsed slicknav_parent"><a href="#" role="menuitem" aria-haspopup="true" tabindex="-1" class="slicknav_item slicknav_row" style="outline: none;">
 +
                        <a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus" tabindex="-1">BaKillus</a>
 +
                        <span class="slicknav_arrow">►</span></a><ul role="menu" aria-hidden="true" class="slicknav_hidden" style="display: none;">
 +
                            <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#sensing" role="menuitem" tabindex="-1">sensing</a></li>
 +
                            <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#adhesion" role="menuitem" tabindex="-1">adhesion</a></li>
 +
                            <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#killing" role="menuitem" tabindex="-1">killing</a></li>
 +
                            <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#suicide" role="menuitem" tabindex="-1">suicide</a></li>
 +
                        </ul>
 +
                    </li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/BioBrickBox" role="menuitem" tabindex="-1"><span class="bacillus">Bacillus</span> BioBrickBox 2.0</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/Modeling" role="menuitem" tabindex="-1">Modeling</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/B_subtilis" class="bacillus" role="menuitem" tabindex="-1">B. subtilis</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="slicknav_collapsed slicknav_parent"><a href="#" role="menuitem" aria-haspopup="true" tabindex="-1" class="slicknav_item slicknav_row" style="outline: none;">
 +
                <a href="https://2014.igem.org/Team:LMU-Munich/Results">Results</a>
 +
                <span class="slicknav_arrow">►</span></a><ul role="menu" class="slicknav_hidden" style="display: none;" aria-hidden="true">
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Results" role="menuitem" tabindex="-1">Results</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Results/Registry" role="menuitem" tabindex="-1">Registry</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Results/Judging" role="menuitem" tabindex="-1">Judging</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="slicknav_collapsed slicknav_parent"><a href="#" role="menuitem" aria-haspopup="true" tabindex="-1" class="slicknav_item slicknav_row" style="outline: none;">
 +
                <a href="https://2014.igem.org/Team:LMU-Munich/Notebook/Protocols">Notebook</a>
 +
                <span class="slicknav_arrow">►</span></a><ul role="menu" class="slicknav_hidden" style="display: none;" aria-hidden="true">
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Notebook/Safety" role="menuitem" tabindex="-1">Safety</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Notebook/Protocols" role="menuitem" tabindex="-1">Protocols</a></li>
 +
                    <!--<li><a href="https://2014.igem.org/Team:LMU-Munich/Notebook/Timeline">Timeline</a></li>-->
 +
                </ul>
 +
            </li>
 +
            <li class="slicknav_collapsed slicknav_parent"><a href="#" role="menuitem" aria-haspopup="true" tabindex="-1" class="slicknav_item slicknav_row" style="outline: none;">
 +
                <a href="https://2014.igem.org/Team:LMU-Munich/Application">Application</a>
 +
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Human_Practice/SynBio" role="menuitem" tabindex="-1">SynBio Day</a></li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Rathenau" role="menuitem" tabindex="-1">Rathenau Instituut</a></li>
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                    <li>
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                        <a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus">BaKillus</a>
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                        <ul>
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                            <li id="inpage-nav-sensing"><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#sensing">sensing</a></li>
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                            <li id="inpage-nav-adhesion"><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#adhesion">adhesion</a></li>
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                        </ul>
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                    </li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/BioBrickBox"><span class="bacillus">Bacillus</span> BioBrickBox 2.0</a></li>
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                <a href="https://2014.igem.org/Team:LMU-Munich/Results">Results</a>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Rathenau">Rathenau Instituut</a></li>
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        </ul>
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                        <ul>
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                            <li id="inpage-nav-sensing"><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#sensing" class="sub-nav-item">sensing</a></li>
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                            <li id="inpage-nav-adhesion"><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#adhesion" class="sub-nav-item">adhesion</a></li>
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                            <li id="inpage-nav-killing"><a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus#killing" class="sub-nav-item">killing</a></li>
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 +
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                    </li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Project/BioBrickBox" class="sub-nav-item"><span class="bacillus">Bacillus</span> BioBrickBox 2.0</a></li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Application/Expertise" class="sub-nav-item">Expertise</a></li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Human_Practice/Meetups" class="sub-nav-item">Meet ups</a></li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Human_Practice/iGEM_School" class="sub-nav-item">iGEM@school</a></li>
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                    <li><a href="https://2014.igem.org/Team:LMU-Munich/Human_Practice/SynBio" class="sub-nav-item">SynBio Day</a></li>
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<div id="home-abstract">
  
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<h1> <span class="mw-headline" id="Abstract">Abstract</span></h1>
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<p>Increasing bacterial resistance to classical antibiotics remains a serious threat and urges the development of novel pathogen-killing strategies. Exploiting bacterial communication mechanisms such as quorum sensing is a promising strategy to specifically target certain pathogens. The major aim of this project is the introduction of a genetic circuit enabling <i>Bacillus subtilis</i> to actively detect, attach to, and eventually kill <i>Staphylococcus aureus</i> and <i>Streptococcus pneumoniae</i>.  
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</p><p>Initially, we will introduce the autoinducer-sensing two-component systems of <i>S. aureus</i> and <i>S. pneumoniae</i> into <i>B. subtilis</i>.  to create a pathogen-detecting strain. By utilizing quorum sensing-dependent promoters, we will then trigger pathogen-killing strategies like the production of antimicrobial peptides or biofilm degradation. As a safety measure a delayed suicide-switch guarantees non-persistence of genetically modified <i>B. subtilis</i> in the absence of pathogens. We envision the use of BaKillus as a smart, cheap and simple-to-use medical device for diagnostics and targeted treatment of multiresistant super bugs.
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 +
<h2> <span class="mw-headline" id="Hi_there.21"> Hi there! </span></h2>
 +
<p>Welcome to our Wiki! I'm BaKillus, the pathogen-hunting microbe, and I'll guide you on this tour through our project. If you want to learn more about a specific step, you can simply close the tour and come back to it anytime you like. So let's start!
  
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<h2> <span class="mw-headline" id="What.27s_the_problem.3F"> What's the problem? </span></h2>
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<p>First of all, what am I doing here? The problem is, pathogenic bacteria all around the world are becoming more and more  resistant against antimicrobial drugs. One major reason for the trend is the inappropriate use of drugs. With my BaKillus super powers, I want to reduce this misuse and thus do my part to save global health.
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<h2> <span class="mw-headline" id="Sensing_of_pathogens"> Sensing of pathogens </span></h2>
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<p>To combat the pathogenic bacteria, I simply eavesdrop on their communication. Bacteria talk with each other via quorum sensing systems, which I use to detect them and trigger my responses.  
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<h2> <span class="mw-headline" id="Adhesion"> Adhesion </span></h2>
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<p>The more specific and effective I can use my powers, the lower the danger is of provoking new resistance development. So I catch pathogens whenever I get hold of them and stick to them until my work is done.
  
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<p></p>
 +
<h2> <span class="mw-headline" id="Killing"> Killing </span></h2>
 +
<p>Talking about my work - killing pathogens is finally what I am made for. In response to quorum sensing molecules of the pathogens, I export a range of antimicrobial substances leading to dissipation of biofilms and the killing of the targeted bacteria.
  
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<p></p>
 +
<h2> <span class="mw-headline" id="Suicide_switch"> Suicide switch </span></h2>
 +
<p>When the job is done and all the bad guys are finished, you don't need a super hero anymore. So after fulfilling my work I say goodbye to the world by activating my suicide switch.
  
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<h2> <span class="mw-headline" id="Application"> Application </span></h2>
 +
<p>Of course I'm not only a fictional hero, but a very real one. In two different prototypes, I could be used for diagnosis or treatment of pathogen-caused diseases. However, there is still a whole lot of regulational and economical questions that have to be answered before.
  
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<h2> <span class="mw-headline" id="See_you.21"> See you! </span></h2>
−
InterLab
+
<p>So now you know my short story - and it is time for me to return to my fight for a safer world. Feel free to take a closer look on my <a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus" class="external text" rel="nofollow">super powers</a>, the <a href="https://2014.igem.org/Team:LMU-Munich/Notebook/Timeline" class="external text" rel="nofollow">process of my development</a> or the plans for a <a href="https://2014.igem.org/Team:LMU-Munich/Application" class="external text" rel="nofollow">medical application</a>.
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<h1>Example</h1>
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Revision as of 23:58, 28 September 2017

Team:LMU-Munich - 2014.igem.org

Team:LMU-Munich

From 2014.igem.org


Abstract

Increasing bacterial resistance to classical antibiotics remains a serious threat and urges the development of novel pathogen-killing strategies. Exploiting bacterial communication mechanisms such as quorum sensing is a promising strategy to specifically target certain pathogens. The major aim of this project is the introduction of a genetic circuit enabling Bacillus subtilis to actively detect, attach to, and eventually kill Staphylococcus aureus and Streptococcus pneumoniae.

Initially, we will introduce the autoinducer-sensing two-component systems of S. aureus and S. pneumoniae into B. subtilis. to create a pathogen-detecting strain. By utilizing quorum sensing-dependent promoters, we will then trigger pathogen-killing strategies like the production of antimicrobial peptides or biofilm degradation. As a safety measure a delayed suicide-switch guarantees non-persistence of genetically modified B. subtilis in the absence of pathogens. We envision the use of BaKillus as a smart, cheap and simple-to-use medical device for diagnostics and targeted treatment of multiresistant super bugs.

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