Please choose a team
2.- About our Lab
This part of the form is for you to tell us about your laboratory space and your safety equipment. If you need help, please consult your faculty advisor or laboratory manager. You can also read the
Safety page and the Risk Groups page for basic information about different types of biology labs.
1. Please upload a photo or two of your lab to the iGEM 2017 server (include your team name in the file name), preferably showing the relevant safety features and paste the link here:
2. What is the Safety Level of your lab?
[Help about Risk Groups and Safety Levels]
3 Which work areas do you use to handle biological materials? Please check all that apply.
4. Have your team members received any safety training yet?
5. Please briefly describe the topics that you learned about (or will learn about) in your safety training.
6. Who provided your safety training? Did you get training from multiple people?
7. Who is responsible for the safety of biology labs at your institution? What are the guidelines for laboratory biosafety? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
8. In your country / region, what are the laws and regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
9. Are there any areas where you have encountered uncertainties concerning institutional, regional, national or international rules and regulations and whether they are relevant to your lab and/or work?
10. Do you think the design of current regulations is sufficient to ensure safe and ethical practices?
(If not, how else could you approach the design? We’re interested in your ideas for strategies that could be used to promote safe and ethical practices as it becomes easier to engineer biology (i.e. monitoring people or information, building safety into the design of equipment, etc). Can you think of any useful examples from other fields?)
3.- About our Project
This part of the form is for you to tell us about your primary project idea, what organisms and parts you will use and what your project will do. We know that iGEM teams often change project topics during the summer. When you change your primary project idea, please update this form to tell us about your new idea!
If you are still working on multiple project ideas by June 30, you may choose one to describe on this form, or you may tell us about other ideas in the "Further Comments" section.
11. How will your project work?
Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)
Good example answers:
"Our bacteria will be engineered to interact with human cells. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."
"Our algae will receive gasses high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2 from the gas."
Bad example answers (not enough detail):
"We are engineering E. coli to cure liver cancer."
"Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."
12. How would your project be used in the real world?
Imagine that your project was fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes and expand in the comments section. (Note: iGEM teams
should not release modified organisms into the natural environment.)
13. What safety or ethical risks would be involved with such a use?
14. Does your project include the use of gene drives?
15. Does your project include the use of animals?
16. Does your project include the use of anti-microbial resistance factors? For more information see the
Safety Policy page and the White List.
Parts and organisms not on the Whitelist
17. Does your project include parts or organisms not on the
18. What is your chassis organism?
Check all species you are genetically modifying in your project.
Other organisms (or parts from them)
19. Besides your chassis, do you plan to use any other organisms (or parts from them)?
What organisms, and what experiments will you do? Please explain briefly. Please include the names of species / cell lines / strains.
This includes the origins of parts taken from other organisms, even if they are being synthesized rather than isolated from nature – you need not include any parts already in the registry.
"Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."
"We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."
"Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."
“We are interested in a RNA-binding protein expressed in Kluyveromyces lactis. We have found the sequence in a paper and will have is synthesized by a provider.”
20. Are there parts of your project which you think may have ethical, safety or security concerns that are not fully covered by current rules and standards?
21. Who have you worked with to resolve any uncertainties or gaps in how you ensure the safety of your project and how difficult have they been to contact?
Beyond this point, no more is required for the June 30th deadline. No need to click submit or fill in more information
4.- New Parts
This part of the of the form is for you to tell us about the parts you have developed during your project. It summarises information that might already have been submitted through check-in forms.
Please visit this
page to download a blank copy of the spreadsheet for this question. (If you need a CSV version instead of XLSX, visit this page.)
Complete the spreadsheet. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.
You may omit non-protein-coding parts (except if they are known virulence factors – you should undertake a literature search to determine if they are), and you may omit parts that were already in the Registry if you are using them without significant modifications. For more information on virulence factors see the Safety Policy page and the White List. Please contact the Safety Committee by emailing safety AT igem DOT org
Click here to show/hide instructions for completing the spreadsheet
Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".
Species name (including strain): For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.
Risk Group: Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)
Risk Group Source: Cite the source from which you obtained the Risk Group information. See Risk Group Guide for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.
Disease risk to humans?: Does this organism cause any disease in humans? If yes, what disease does it cause?
Part number/name: For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.
Natural function of part: For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.
How did you acquire it?: Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)
How will you use it?: Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")
Notes: Use this column to give any additional information that is necessary.
[File: -- Please do not change the "Destination Filename"! Upload Spreadsheet TeamName Safety2017 Spreadsheet.xls]
You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.
Click here to VIEW your spreadsheet
5.- Sign Off
Only a team Instructor or PI may submit the Safety Form.
Instructors/PIs, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Final Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.
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