BioSensor Lab Results

As we were unable to transform the construct, therefore, the results for Salt Sensor testing were obtained from iGEM Pitt as a part of the collaboration

1. The results for the RFP fluorescence measured for three replicated of the blank as well as two constructs at excitation 585nm/emission 607nm.

NFkB (negative control)	0mM	50mM	100mM	200mM	400mM	800mM	1600mM
1	326525	322701	324569	324359	329257	424215	447835
2	324213	321232	323679	324437	326850	421070	436049
3	321979	321615	322685	324569	327046	399897	406696

Wildtype	0mM	50mM	100mM	200mM	400mM	800mM	1600mM
1	322379	324207	326546	324794	330924	341689	388728
2	322539	323331	326077	324704	331310	367254	438918
3	322369	323430	326386	324750	330914	353266	381339

Consensus	0mM	50mM	100mM	200mM	400mM	800mM	1600mM
1	326742	326016	325673	329309	337418	377574	378246
2	325087	326368	326416	327722	337804	367197	400206
3	325689	324992	325312	327109	334483	364028	373127

2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs.

		0mM	50mM	100mM	200mM	400mM	800mM	1600mM
Average	NFkB	324239	321849.33	323644.33	324455	327717.7	415060.7	430193.3
	Consensus	325839.33	325792	325800.33	328046.7	336568.3	369599.7	383859.7
	Wildtype	322429	323656	326336.33	324749.3	331049.3	354069.7	402995

NFkB	Consensus	1600.3333	3942.6667	2156	3591.667	8850.667	-45461	-46333.7
NFkB	WildType	-1810	1806.6667	2692	294.3333	3331.667	-60991	-27198.3

3. The normalized fluorescence values for the two constructs at different NaCl concentrations.

NaCl Concentration (mM)	Wildtype	Consensus
0	-1810	1600.33
50	1806.67	3942.67
100	2692	2156
200	294.33	3591.67
400	3331.67	8850.67
800	-60991	-45461
1600	-27198.33	-46333.67

Team:CMUQ/BioSensor Lab R

BioSensor Lab Results