Team:CMUQ/Dspb Lab R
Dspb Lab Results
The following results were obtained for the Dispersin B, which is an improvement on an Existing part:
1. The results for the GFP fluorescence measured for three replicates of the LB (blank) as well as two constructs at excitation 585nm/emission 607nm
| Average | SD | t test | ||||
|---|---|---|---|---|---|---|
| LB | -0.004 | -0.0013 | -0.055 | -0.0201 | 0.030254421 | |
| t=0 supernatant | -0.034 | 0.009 | 0.005 | 0.006666667 | 0.023755701 | 0.328585241 |
| t=16 supernatant | 0.153 | 0.038 | 0.046 | 0.079 | 0.064210591 | 0.050130586 |
2. Averaging and normalizing the fluorescence readings measured from culture before pelleting the cells and then after pelleting the cells.
| Fluorescence | |||||
|---|---|---|---|---|---|
| pDP12 | -0.033 | 0.02 | 0.036 | 0.007666667 | 0.036115555 |
| cells t=0 | -0.029 | 0.008 | -0.008 | -0.009666667 | 0.018556221 |
| cells t=16 | 0.09 | 0.032 | 0.07 | 0.064 | 0.02946184 |
| supernatant t=0 | 0 | -0.145 | 0.0065 | -0.046166667 | 0.085653858 |
| supernatant t=16 | 0.048 | 0.122 | 0.045 | 0.071666667 | 0.043615746 |
| Norm. cells t=0 | 0.004 | -0.012 | -0.044 | -0.017333333 | 0.024440404 |
| Norm. cells t=16 | 0.123 | 0.012 | 0.034 | 0.056333333 | 0.058773577 |
| Norm. supernatant t=0 | 0.033 | -0.165 | -0.0295 | -0.053833333 | 0.101217999 |
| Norm. supernatant t=16 | 0.081 | 0.102 | 0.009 | 0.064 | 0.048774994 |
