Team:Mingdao/InterLab

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Interlab

In order to meet the requirement of bronze medal, our team conducted the Interlab Study experiments, including transformation, serial dilution and florescence measurement, etc. Throughout the Interlab Study, we not only learned the concept of accurate measurement but also improved our experiment skills, which is a great preparation for us before our project get started!

Lab Environment

 
 

Material

  • Positive Control (BBa_I20270)
  • Negative Control (BBa_R0040)
  • Test Device 1 (BBa_J364000)
  • Test Device 2 (BBa_J364001)
  • Test Device 3 (BBa_J364002)
  • Test Device 4 (BBa_J364003)
  • Test Device 5 (BBa_J364004)
  • Test Device 6 (BBa_J364005)
  • 1ml LUDOX
  • ddH20
  • 96 well plate
  • fluorescein
  • 10ml 1xPBS
  • Plate Reader: Synergy H1 Hybrid Multi-Mode Reader
  • Media: Luria Bertani
  • 50ml colonial tube

    • Result

    OD600 Reference Point Measurement

       Calculation for correction factor between Abs600 and OD600

    Fluorescein standard curve

    • Gain:   45
    • Emission wavelength:    488nm
    • Excitation wavelength:    518nm
    • Optics:    top

    Result

    OD600 Reference Point Measurement

       Calculation for correction factor between Abs600 and OD600
       Plot of Fluorescein Standard Curve
       Plot of Fluorescein Standard Curve

    - Cell Measurement -

    Plot of Raw Abs600 measurement


    Plot of Raw Fluorescence measurement

    Experiment Photos

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