Team:SCUT-China B

2017 SCUT_China_B IGEM

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Abstract

Awarded GOLD medal at the 2017 MIT

Nominated for:

Best Wiki

Best Presentation

Best Education & Public Engagement

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We were working in a new lab that some of it is still being fitting up. Workers used scaffold to install facilities high on the ceiling. Suddenly I came up with an interesting idea that what if the scaffold is construct by protain. Then I realized that scaffold is such a regular network that we could carry something on it in the order we like. If the “something” were enzymes of co-catalyze system, we got a perfectly uniform distributed immobilized enzyme of co-catalysis system. If the “something” were cells the scaffold can provide a 3D microenvironment for them and we got a scaffold for tissue engineering.

So now how do we construct the scaffold. We knew that there was many protein self-assembly system but most of them are assembled irregularly. But just like constructing a real scaffold we can use different linkage to link our materials altogether forming a network. After research on literatures, we noticed two widely used self-assembly system in hydrogel: spy system and coiled coil system and our next problem is how to combine these two kinds of linkage

Synthetic biology has long been working hard on gene circuits and switches ignoring another important biomacromolecule proteins. The modularization of proteins is more difficult than DNA, because of the complex interaction between different parts on their structure and function. But as technology developing, we are learning more and more about the relationship between protein’s structure and function. Now we can combine different proteins with special amino acid sequence like GS linker without hurting its function. So now it is time to get down to the modularization of proteins with which we will be able to design proteins itself for new function. For so long the combination of genes are rely on soldered dot of special restriction Enzyme cutting site. These soldered dots might influence to the function of protein “bricks” after combination. But now with assembly PCR and overlap PCR we can link gene sequences traceless and the proteins they expressed as well. Therefore, we have a way to combine to system that have been widely used in hydrogel to construct our “scaffold”.

Since the proteins in the system are hydrophilic molecules our scaffold is actually a hydrogel.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.

The only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.