Team:TUDelft/Main-Parts

When constructing our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts we synthesised contain the coding sequence, RBS, promoter and terminators, if applicable. Therefore, these parts are composite. The different basic parts comprising the composite parts are documented separately, and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. These basic parts were generated by using PCR and restriction ligation on the synthesised composite parts. We created a part collection comprised of 4 composite parts encoding for different Tardigrade Proteins (TDPs) and the 4 basic parts of these genes, all with elaborate characterisation so the user may choose the best protein for their project accordingly. For the composite parts of the TDPs we designed a new, strong RBS. This part does not exist separately. Additionally, we include the documentation of a basic part encoding the gene for Cas13a, derived from the plasmid pC011 from the authors of Gootenberg et al. 2017. This BioBrick does not exist separately. Finally, we submitted a composite part containing a nonsense spacer with flanking double repeats (with constitutive promoter), appropriate for use for Cas13a. This part is designed so that the user can easily change the spacer according to the sequence they wish to target. Find more info on this cool part on its page in the registry! We also documented this as a basic part without a promoter. This part does not exist separately. Our favourite basic part is BBa_K2306003 and our favourite composite part is BBa_K2306008.

Improved BioBrick / Further Characterisation

In our project, we added to the characterisation of an existing Biobrick, the TolR, periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TorA-GFP composite part was based on the BioBrick created by NTU Trontheim with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.

All Parts

Fave Part Name Type Description Designer Length
BBa_K2306000 Coding TorA-GFP fusion Gabriella Tany 846
BBa_K2306001 Composite TorA-GFP fusion (LacI promoter) Gabriella Tany 1212
BBa_K2306002 Coding Secretory-abundant heat soluble protein 68234 (SAHS 68234) Guillermo Serena Ruiz 525

♥

 BBa_K2306003 Coding Secretory-abundant heat soluble protein 33020 (SAHS 33020) Guillermo Serena Ruiz 509
BBa_K2306005 Coding Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) Guillermo Serena Ruiz 684
BBa_K2306006 Coding Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) Guillermo Serena Ruiz 681
BBa_K2306007 Composite Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator) Guillermo Serena Ruiz 768
♥ BBa_K2306008 Composite Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator) Guillermo Serena Ruiz 752
BBa_K2306010 Composite Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator Guillermo Serena Ruiz 927
BBa_K2306011 Composite Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator) Guillermo Serena Ruiz 924
BBa_K2306012 Coding Cas13a Jeroen Jacques 3920
BBa_K2306013 Composite Cas13a spacer with flanking double repeats, constitutive promoter Jeroen Jacques and Jasper Veerman 134
BBa_K2306014 RBS RBS from bacteriophage T7 gene 10 and Shine Dalgarno Guillermo Serena Ruiz 30
BBa_K2306015 Other Cas13a spacer with flanking double repeats Jeroen Jacques and Jasper Veerman 99

Basic Parts

Fave Part Name Type Description Designer Size (bp)
BBa_K2306002 Basic Secretory-abundant heat soluble protein (SAHS) 68234 Guillermo Serena Ruiz 525
♥ BBa_K2306003 Basic Secretory-abundant heat soluble protein (SAHS) 33020 Guillermo Serena Ruiz 509
BBa_K2306005 Basic Cytosolic-abundant heat soluble protein (CAHS) 106094 Guillermo Serena Ruiz 684
BBa_K2306006 Basic Cytosolic-abundant heat soluble protein (CAHS) 94205 Guillermo Serena Ruiz 681
BBa_K23006000 Basic TorA GFP Gabriella Tany 681
BBa_K2306012 Basic Cas13a Jeroen Jacques 3456
BBa_K2306014 RBS RBS from bacteriophage T7 gene 10 and Shine Dalgarno Guillermo Serena Ruiz 30
BBa_K2306015 Basic Spacer with flanking double repeats for Cas13a Jeroen Jacques, Jasper Veerman 99

Composite Parts

Fave Part Name Type Description Designer Size (bp)
BBa_K2306007 Composite Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 833
♥ BBa_K2306008 Composite Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 817
BBa_K2306010 Composite Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 992
BBa_K2306011 Composite Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 989
BBa_K2306001 Composite TorA GFP fusion with inducible LacI promoter, RBS and double terminator Gabriella Tany 681
BBa_K2306015 Composite Cas13a spacer with flanking double repeats, constitutive promoter Jeroen Jacques, Jasper Veerman 134

Part Collection

Fave Part Name Type Description Designer Size (bp)
BBa_K2306007 Composite Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 833
♥ BBa_K2306008 Composite Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 817
BBa_K2306010 Composite Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 992
BBa_K2306011 Composite Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator Guillermo Serena Ruiz 989
BBa_K2306002 Basic Secretory-abundant heat soluble protein (SAHS) 68234 Guillermo Serena Ruiz 525
♥ BBa_K2306003 Basic Secretory-abundant heat soluble protein (SAHS) 33020 Guillermo Serena Ruiz 509
BBa_K2306005 Basic Cytosolic-abundant heat soluble protein (CAHS) 106094 Guillermo Serena Ruiz 684
BBa_K2306006 Basic Cytosolic-abundant heat soluble protein (CAHS) 94205 Guillermo Serena Ruiz 681