Team:XJTLU-CHINA/Protocols Ligation

Ligation

Procedure:

1. Add 2μl digested plasmid backbone (25ng).
2. Add equimolar digested fragment.
3. Add 1μl T4 DNA ligase buffer.
4. Add 0.5μl T4 DNA ligase.
5. Add ddH₂O to 10ul volume.
6. Ligate at 16℃ for 30 minutes. Heat kill at 80℃ for 20 minutes.
7. Transform 1-2μl of the product.

Collaborators and Supporters

Location

Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123

Social

Get in touch

igem@xjtlu.edu.cn

XJTLU-CHINA iGEM 2017