Team:iTesla-SoundBio/Notebook
Notebook
Date: 5/28/2017
- Amplified pcbA1 with normal primers
- MEA, JA
Date: 6/04/2017
- Ran gel for future purification on Gibson PCRs conducted last training session, and isolated pcbA1 DNA
- KZ, MEA, JJW
Date: 6/23/2017
- Amplified genes pcbA4 and pcbA5 using PCR
- RAN, JA
Date: 6/26/2017
- PcbA5 & pcbA4 PCR product Gel Analyzation & Purification, Restriction Digest of pET-29B
- RAN, JA, MEA, JJW
Date: 6/30/2017
- Ran analyzation and purification gels on digested pET-29B plasmid
- RAN, JA, MEA, JJW
Date: 7/01/2017
- Conducted gel extraction on the pET-29B plasmid and pcbA4 gblock that were caught in previous gel electrophoresis slices
- JJW, MEA
Date: 7/08/2017
- Used a nanodrop to measure purity and concentration of DNA in digested pET-29B and pcbA4 samples that underwent gel purification
- KZ
Date: 7/10/2017
- pcbA4 pET-29B Gibson Assembly MEA, JA, JJW, KZ
Date: 7/13/2017 Transforming Plasmids into Purchased Competent Cells
- MEA, JJW, KZ
Date: 7/16/2017
- Inoculating Transformed Purchased Cells into LB+kan Broth
- ZM, MEA
- Transforming pcbA4 Plasmids into Self-Made Competent Cells
- KZ, MEA
Date: 7/18/2017
- Inoculating Transformed Self-Made Cells and pET-29B Plasmids
- JA, MEA, KZ
Date: 7/23/2017
- Miniprep of Inoculated Cells and Nanodrop Verification
- MEA, JA, KZ
Date: 7/25/2017
- Digesting pET-29B Plasmids
- KZ
Date: 7/30/2017
- Gel Purifying Digested pET-29B and Inoculating Transformed Cells
- MEA, KZ
Date: 7/31/2017
- Gel Extraction of Digested pET-29B and Nanodrop Verification
- MEA, KZ
Date: 8/1/2017
- Gibson Assembly pcbA4 gene (insert) into digested pET-29B plasmid (vector)
- KZ
Date: 8/5/2017
- Transformation of Gibson Assemblied pcbA4 Plasmids into Self Made Competent Cells
- MEA
Date: 8/8/2017
- Creating Carb plates
- JJW
Date: 8/9/2017
- PCR to amplify pcbA4 geneblock
- JJW
Date: 8/11/2017
- Colony PCR on transformed e.coli cells containing pcbA4
- KZ
Date: 8/12/2017
- Gel analysis on our plasmids that have underwent colony PCR
- KZ, RAN
Date: 8/14/2017
- Gel electrophoresis on PCRed pcbA4 gblock and colony PCR trial #7 and #8
- JJW
Date: 8/19/2017
- Prepping DH5 alpha Cells for Transformation
- JJW, MEA
Date: 8/20/2017
- Gibson Assembly and Transformation of Positive Control
- KZ
Date: 8/30/2017
- Amplifying Geneblock with PCR and Gel Analyzation of PCR
- MEA, KZ
Date: 8/31/2017
- Digesting pET-29B Plasmids and Gel Analysis
- MEA, KZ
Date: 9/10/2017
- Amplify all three PCB geneblocks through PCR and purify the PCRed solutions through PCR purification
- MEA, SA, JJW, KZ
Date: 9/12/2017
- Pet-29b and Biobrick Backbone Miniprep, Analytical Gel on PCR geneblocks and vectors
- JA, MEA
Date: 9/15/2017
- Restriction Digest on PCRed A1, 4, 5 and Miniprepped Vectors/ PCR on A4/5 Promoter and A5 gblock
- MEA, JJW, MS
Date: 9/20/2017
- PCR Purify and Gel Analyze pcbA5 and A4/5 Promoter, Gel Purify Digested Tubes
- MEA, JA, AC, JJW, KZ
Date: 9/27/2017
- Gel Extracting Biobrick Ingredients and Digesting Promoter and pcbA5
- MEA, JA, CL, JJW, KZ
Date: 9/29/2017
- Gel Purify and Gel Extract A4/5 Promoter, pcbA5, and pSB1C3
- JJW, MEA, SA
Date: 10/4/2017
- Ligation and Transformation of bb and e Backbones and gblocks
- JJW, JA, MEA
Date: 10/8/2017
- Miniprep of Transformed RFP Backbone Colonies
- JJW, KT, MS
Date: 10/11/2017
- Restriction Digest on RFP and Running Purification Gel, Preparing Mini Preps for Sequencing
- MEA, CA, AC, JJW, JA
Date: 10/13/2017
- Gel Extraction of RFP, PCR Promoter and AmilGFP with primers, Making Antibiotic Plates
- JJW, MEA
Date: 10/17/2017
- New PCR on A5 and Promoter Inserts
- JA
Date: 10/18/2017
- Ligation and Transformation of Plan A, Digest and Gel Purification of Plan B, Off-the-cuff Digest of Promoter and RFP
- JJW, JA, CL, MEA
Date: 10/20/2017
- Miniprepping Successfuly Transformed Colonies
- JJW, MEA, JA, SA
Date: 10/21/2017
- Preparing Miniprep Samples for Sequencing
- JA, JJW
