Difference between revisions of "Team:Aix-Marseille/Bacteriophages"
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{{Aix-Marseille|title=Engineering bacteriophages|toc=__TOC__}} | {{Aix-Marseille|title=Engineering bacteriophages|toc=__TOC__}} | ||
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| + | Bacteriophages play a special role in nanoscale cargo-delivery developments, because they can be regarded as naturally occurring nanomaterials. Viral nanoparticles (VNPs), in particular bacteriophages, are attractive options for cargo-delivery as they are biocompatible, biodegradable, and non-infectious to mammals. | ||
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| + | Phage systems, like M13, have been employed in biotechnological applications, most prominently in the identification and maturation of medically-relevant binding molecules through phage display. The application of phages in materials and nanotechnology is mainly due to their nanoscale size and simple life cycles. We choose to use those application in our advantage in order to target Xylella fastidiosa and other pathogenic bacteria. | ||
==M13 phages== | ==M13 phages== | ||
| − | M13 is a filamentous phage that infects E. coli that carry the F-episome. Active infection with M13 does not kill the host cell. | + | M13 is a filamentous phage that infects E. coli that carry the F-episome. It is composed of a circular single-stranded DNA molecule encapsided in a thin flexible tube composed of multiple copies of the same protein. Infection starts when the minor coat protein P3 attaches to F pilus of the bacterium. Active infection with M13 does not kill the host cell. |
[[Team:Aix-Marseille/M13|Read more…]] | [[Team:Aix-Marseille/M13|Read more…]] | ||
Revision as of 14:05, 31 August 2017
Engineering bacteriophages
Bacteriophages play a special role in nanoscale cargo-delivery developments, because they can be regarded as naturally occurring nanomaterials. Viral nanoparticles (VNPs), in particular bacteriophages, are attractive options for cargo-delivery as they are biocompatible, biodegradable, and non-infectious to mammals.
Phage systems, like M13, have been employed in biotechnological applications, most prominently in the identification and maturation of medically-relevant binding molecules through phage display. The application of phages in materials and nanotechnology is mainly due to their nanoscale size and simple life cycles. We choose to use those application in our advantage in order to target Xylella fastidiosa and other pathogenic bacteria.
M13 phages
M13 is a filamentous phage that infects E. coli that carry the F-episome. It is composed of a circular single-stranded DNA molecule encapsided in a thin flexible tube composed of multiple copies of the same protein. Infection starts when the minor coat protein P3 attaches to F pilus of the bacterium. Active infection with M13 does not kill the host cell.
Phages-like particules design
Bacteriophages are capable of expressing their genomes, and generating new copies of themselves. We choose to limit the phage ability to reproduce itself in order to contain it. As it is possible to produce recombinant viruses that express foreign proteins, it is possible to restrain their capacity to reproduce themself. [1]
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome. They have been applied not only as prophylactic and therapeutic vaccines but also as vehicles in drug and gene delivery and, more recently, as tools in nanobiotechnology. [1]
In this project, we want to creat a M13-like phage like particle, in order to inject toxin in the bacterium.
References
