Difference between revisions of "Team:Cardiff Wales/compositeparts"

Line 25: Line 25:
 
     <td>pGB-A2</td>
 
     <td>pGB-A2</td>
 
     <td>A constitutive promoter with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A constitutive promoter with our TSH antagonist <b>without</b> the his tags for purification</td>
 +
<td>~length</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 32: Line 33:
 
     <td>pGB-A2</td>
 
     <td>pGB-A2</td>
 
     <td>A constitutive promoter with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A constitutive promoter with our TSH antagonist <b>with</b> the his tags for purification</td>
 +
<td>~length</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 39: Line 41:
 
     <td>pGB-A2</td>
 
     <td>pGB-A2</td>
 
     <td>A constitutive promoter with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A constitutive promoter with Luc+ as a coding sequence to analyse expression levels</td>
 +
<td>~length</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 45: Line 48:
 
     <td>NosT</td>
 
     <td>NosT</td>
 
     <td>pGB-A2</td>
 
     <td>pGB-A2</td>
 +
<td>A constitutive promoter with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant</td>
 +
<td>~length</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 51: Line 56:
 
     <td>NosT</td>
 
     <td>NosT</td>
 
     <td>pGB-A2</td>
 
     <td>pGB-A2</td>
 +
<td>A constitutive promoter with XVE as a coding sequence. This produces a protein product that activates the inducible promoter LexA</td>
 +
<td>~length</td>
 +
  </tr>
 +
<tr>
 +
    <td>LexA</td>
 +
    <td>TSH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter that is induced by XVE with our TSH antagonist <b>without</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>LexA</td>
 +
    <td>TSHH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter that is induced by XVE  with our TSH antagonist <b>with</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>LexA</td>
 +
    <td>Luc+</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter that is induced by XVE  with Luc+ as a coding sequence to analyse expression levels</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>LexA</td>
 +
    <td>Lucx5</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
<td>A promoter that is induced by XVE  with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PDF1</td>
 +
    <td>TSH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by jasmonic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PDF1</td>
 +
    <td>TSHH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by jasmonic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PDF1</td>
 +
    <td>Luc+</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by jasmonic acid with Luc+ as a coding sequence to analyse expression levels</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PR2</td>
 +
    <td>TSH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by salicylic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PR2</td>
 +
    <td>TSHH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by salicylic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PR2</td>
 +
    <td>Luc+</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>GST6</td>
 +
    <td>TSH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by salicylic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>GST6</td>
 +
    <td>TSHH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by salicylic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>WRKY30</td>
 +
    <td>Luc+</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>WRKY30</td>
 +
    <td>TSH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist <b>without</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>WRKY30</td>
 +
    <td>TSHH</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist <b>with</b> the his tags for purification</td>
 +
<td>~length</td>
 +
  </tr>
 +
  <tr>
 +
    <td>WRKY30</td>
 +
    <td>Luc+</td>
 +
    <td>NosT</td>
 +
    <td>pGB-A2</td>
 +
    <td>A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels</td>
 +
<td>~length</td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>

Revision as of 19:54, 2 September 2017




Composite Parts






Promotor Coding sequence Terminator Plasmid Summary Approximate Length
35S TSH NosT pGB-A2 A constitutive promoter with our TSH antagonist without the his tags for purification ~length
35S TSHH NosT pGB-A2 A constitutive promoter with our TSH antagonist with the his tags for purification ~length
35S Luc+ NosT pGB-A2 A constitutive promoter with Luc+ as a coding sequence to analyse expression levels ~length
35S Lucx5 NosT pGB-A2 A constitutive promoter with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant ~length
35S XVE NosT pGB-A2 A constitutive promoter with XVE as a coding sequence. This produces a protein product that activates the inducible promoter LexA ~length
LexA TSH NosT pGB-A2 A promoter that is induced by XVE with our TSH antagonist without the his tags for purification ~length
LexA TSHH NosT pGB-A2 A promoter that is induced by XVE with our TSH antagonist with the his tags for purification ~length
LexA Luc+ NosT pGB-A2 A promoter that is induced by XVE with Luc+ as a coding sequence to analyse expression levels ~length
LexA Lucx5 NosT pGB-A2 A promoter that is induced by XVE with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant ~length
PDF1 TSH NosT pGB-A2 A promoter induced by jasmonic acid with our TSH antagonist without the his tags for purification ~length
PDF1 TSHH NosT pGB-A2 A promoter induced by jasmonic acid with our TSH antagonist with the his tags for purification ~length
PDF1 Luc+ NosT pGB-A2 A promoter induced by jasmonic acid with Luc+ as a coding sequence to analyse expression levels ~length
PR2 TSH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist without the his tags for purification ~length
PR2 TSHH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist with the his tags for purification ~length
PR2 Luc+ NosT pGB-A2 A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels ~length
GST6 TSH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist without the his tags for purification ~length
GST6 TSHH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist with the his tags for purification ~length
WRKY30 Luc+ NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels ~length
WRKY30 TSH NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist without the his tags for purification ~length
WRKY30 TSHH NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist with the his tags for purification ~length
WRKY30 Luc+ NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels ~length