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<h2> Week 4 (24/7/17-28/7/17)</h2> | <h2> Week 4 (24/7/17-28/7/17)</h2> | ||
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+ | <td> <top> <img class="media object pull-left" src="https://static.igem.org/mediawiki/2017/c/c8/Notepadlogo.jpg"> </top> </td> | ||
+ | <td> <h4> Dry Lab </h4> | ||
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<li> Unofficial team meeting took place as some team members still absent. </li> | <li> Unofficial team meeting took place as some team members still absent. </li> | ||
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<h2> Week 5 (31/7/17-4/7/17)</h2> | <h2> Week 5 (31/7/17-4/7/17)</h2> | ||
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+ | <td> <top> <img class="media object pull-left" src="https://static.igem.org/mediawiki/2017/c/c8/Notepadlogo.jpg"> </top> </td> | ||
+ | <td> <h4> Dry Lab </h4> | ||
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<li> </li> | <li> </li> |
Revision as of 14:37, 3 September 2017
Notebook
Week 1 (3/7/17-7/7/17)
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Dry Lab
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Week 2 (10/7/17-14/7/17)
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Dry Lab
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Week 3 (17/7/17-21/7/17)
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Dry Lab
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Wet Lab
- We reviewed the results from the restriction enzyme testing experiment conducted last week.
- We interpreted electrophoresis gel results
- XbaI did not perform well
- SpeI appeared to be contaminated
- We discovered that although 3.1 buffer is ideal for Pst when doing double digest it is ideal to use Cutsmart or 2.1.
- New restriction enzymes are expected this week.
- Conducted PCR of three backbones so we would have more stock available (CAM, Kan, Amp).
- Mike re did our digest test of restriction enzymes. Ran electrophoresis gel of PCR’s and digests. Q5 didn’t work at all and KOD did not work for Kan.
- Mike’s digests worked out.
- The changes he used included Smart cut buffer used across all enzymes and digest extended to 90min.
- The team re did the digest and re did Kan PCR again. Ran gel again. Digests look great but still no success with Kan PCR. Ed has suggested we try again with a lower temp for annealing as it’s possible our anneal temp was too close to the primer melt temp. He has suggested that the CAM and Amp pcr’s were not very successful either considering the main bands were weak and primer dimer bands strong.
- We grew overnight subculture of three colonies from each of three plates- Fer, Hyd1, HydEF. The next day we extracted the plasmid using miniprep (Qiagen). We digested these using EcoRI as well.
Week 4 (24/7/17-28/7/17)
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Dry Lab
Wet Lab
Week 5 (31/7/17-4/7/17)
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