Difference between revisions of "Team:Kyoto/Experiments"

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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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  <header>
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      <div id="nav-wrapper">
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        <ul class="nv">
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            <li id="nv0"><a href="https://2017.igem.org/Team:Kyoto">Home</a></li>
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            <li id="nv1"><a href="https://2017.igem.org/team:Kyoto/Project">Project</a></li>
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            <li id="nv2"><a href="https://2017.igem.org/Team:Kyoto/Experiments">Experiments</a></li>
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            <li id="nv3"><a href="https://2017.igem.org/Team:Kyoto/Achievements">Achievements</a></li>
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            <li id="nv4"><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li>
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            <li id="nv5"><a href="https://2017.igem.org/Team:Kyoto/Practice">Practice</a></li>
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            <li id="nv6"><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a></li>
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            <li id="nv7"><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li>
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        </ul>
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      </div>
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  </header>
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  <h1>Material&amp;Methods</h1>
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  <ol>
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      <li><a href="#Parts">Parts</a></li>
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      <li><a href="#Primer">Primer list</a></li>
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      <li><a href="#Materials">Materials</a></li>
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        <ul>
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            <li><a href="#Kit">3-1 Kit</a></li>
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            <li><a href="#Enzyme">3-2 Restricton Enzyme</a></li>
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            <li><a href="#Polymerase">3-3 Polymerase</a></li>
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            <li><a href="#Ligase">3-4 DNA ligase</a></li>
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            <li><a href="#Marker">3-5 Marker</a></li>
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            <li><a href="#Organism">3-6 Organism</a></li>
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            <li><a href="#Antibiotics">3-7 Antibiotics</a></li>
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            <li><a href="#Equipment">3-8 Equipment</a></li>
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            <li><a href="#backbones">3-9 Backborns</a></li>
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            <li><a href="#Buffer">3-10 Buffer</a></li>
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            <li><a href="#SDSPAGE">3-11 SDSPAGE/Western blotting</a></li>
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/!-----If something should be added, list it from here-----!/
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        </ul>
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      <li><a href="#Methods">Methods</a></li>
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        <ul>
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            <li><a href="#Miniprep">4-1 Miniprep</a></li>
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            <li><a href="#Gel">4-2 Gel Extraction</a></li>
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            <li><a href="#Digestion">4-3 Restrict Enzyme Digestion</a></li>
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            <li><a href="#Ligation">4-4 Ligation</a></li>
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            <li><a href="#Transformation">4-5 Transformation</a></li>
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            <li><a href="#PCR">4-6 PCR</a></li>
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            <li><a href="#Sequencing">4-7 Sequencing</a></li>
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            <li><a href="#RT-PCR">4-8 RT-PCR</a></li>
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            <li><a href="#Western>4-9 Basic protocol of Western blotting</a></li>
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            <li><a href="#Western2">4-10 Western blotting(Membrane fraction)</a></li>
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            /!------If something should be added, list it-------!/
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        </ul>
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      </li>
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  <h3><a name="#Parts">Parts</a></h3>
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  <h3><a name="#Primer">Primer list</a></h3>
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      <table>
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        <tr>
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            <th>Primer name</th>
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            <th>Sequence</th>
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            <th>Length</th>
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            <th>Tm</th>
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            <th>GC&#037;</th>
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            <th>Designer</th>
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            <th>Manufacturer</th>
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        </tr>
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      </table>
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  <h3><a name="#Materials">Materials</a></h3>
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      <table>
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        <caption><a name="#Kit">Kit</caption>
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        <thead>
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            <tr>
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              <th>Name</th>
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              <th>Supplier</th>
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            </tr>
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        </thead>
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        <tbody>
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            <tr>
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              <td></td>
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              <td></td>
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            </tr>
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        </tbody>
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      </table>
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      <table>
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        <caption><a name="#Enzyme">Restriction Enzyme</caption>
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        <thead>
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            <tr>
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              <th>Name</th>
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              <th>Supplier</th>
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            </tr>
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        </thead>
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        <tbody>
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            <tr>
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              <td></td>
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              <td></td>
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            </tr>
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        </tbody>
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  <h3><a name="#Methods">Methods</a></h3>
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  <footer>
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      <div id="footer_logos">
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        <img src="" width="" height="">
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      </div>
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      <div id="footer_contact">
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        <img src="" width="" height="">
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      </div>
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      <div id="footer_social">
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        <a href="mailto:igemkyoto@gmail.com"><img src=""></a>
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        <a href="https://twitter.com/igemkyoto"><img src=""></a>
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        <a href="https://www.facebook.com/IgemKyoto/"><img src=""></a>
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      </div>
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  </footer>
  
<p>
 
Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
 
</p>
 
 
</div>
 
 
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<h5>What should this page contain?</h5>
 
<ul>
 
<li> Protocols </li>
 
<li> Experiments </li>
 
<li> Documentation of the development of your project </li>
 
</ul>
 
 
</div>
 
 
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<h5>Inspiration</h5>
 
<ul>
 
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
 
<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
 
<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
 
</ul>
 
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Revision as of 10:28, 7 September 2017

Material&Methods

  1. Parts
  2. Primer list
  3. Materials
  4. Methods
  5. Parts

    Primer list

    Primer name Sequence Length Tm GC% Designer Manufacturer

    Materials

    Kit
    Name Supplier

    Methods

    Restriction Enzyme
    Name Supplier