(Prototype team page) |
|||
Line 1: | Line 1: | ||
− | |||
<html> | <html> | ||
− | < | + | <head> |
+ | <link rel="stylesheet" type="text/css" href=""> | ||
+ | </head> | ||
− | < | + | <body> |
− | < | + | <header> |
+ | <div id="nav-wrapper"> | ||
+ | <ul class="nv"> | ||
+ | <li id="nv0"><a href="https://2017.igem.org/Team:Kyoto">Home</a></li> | ||
+ | <li id="nv1"><a href="https://2017.igem.org/team:Kyoto/Project">Project</a></li> | ||
+ | <li id="nv2"><a href="https://2017.igem.org/Team:Kyoto/Experiments">Experiments</a></li> | ||
+ | <li id="nv3"><a href="https://2017.igem.org/Team:Kyoto/Achievements">Achievements</a></li> | ||
+ | <li id="nv4"><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li> | ||
+ | <li id="nv5"><a href="https://2017.igem.org/Team:Kyoto/Practice">Practice</a></li> | ||
+ | <li id="nv6"><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a></li> | ||
+ | <li id="nv7"><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </header> | ||
+ | <h1>Material&Methods</h1> | ||
+ | <ol> | ||
+ | <li><a href="#Parts">Parts</a></li> | ||
+ | <li><a href="#Primer">Primer list</a></li> | ||
+ | <li><a href="#Materials">Materials</a></li> | ||
+ | <ul> | ||
+ | <li><a href="#Kit">3-1 Kit</a></li> | ||
+ | <li><a href="#Enzyme">3-2 Restricton Enzyme</a></li> | ||
+ | <li><a href="#Polymerase">3-3 Polymerase</a></li> | ||
+ | <li><a href="#Ligase">3-4 DNA ligase</a></li> | ||
+ | <li><a href="#Marker">3-5 Marker</a></li> | ||
+ | <li><a href="#Organism">3-6 Organism</a></li> | ||
+ | <li><a href="#Antibiotics">3-7 Antibiotics</a></li> | ||
+ | <li><a href="#Equipment">3-8 Equipment</a></li> | ||
+ | <li><a href="#backbones">3-9 Backborns</a></li> | ||
+ | <li><a href="#Buffer">3-10 Buffer</a></li> | ||
+ | <li><a href="#SDSPAGE">3-11 SDSPAGE/Western blotting</a></li> | ||
+ | /!-----If something should be added, list it from here-----!/ | ||
+ | </ul> | ||
+ | <li><a href="#Methods">Methods</a></li> | ||
+ | <ul> | ||
+ | <li><a href="#Miniprep">4-1 Miniprep</a></li> | ||
+ | <li><a href="#Gel">4-2 Gel Extraction</a></li> | ||
+ | <li><a href="#Digestion">4-3 Restrict Enzyme Digestion</a></li> | ||
+ | <li><a href="#Ligation">4-4 Ligation</a></li> | ||
+ | <li><a href="#Transformation">4-5 Transformation</a></li> | ||
+ | <li><a href="#PCR">4-6 PCR</a></li> | ||
+ | <li><a href="#Sequencing">4-7 Sequencing</a></li> | ||
+ | <li><a href="#RT-PCR">4-8 RT-PCR</a></li> | ||
+ | <li><a href="#Western>4-9 Basic protocol of Western blotting</a></li> | ||
+ | <li><a href="#Western2">4-10 Western blotting(Membrane fraction)</a></li> | ||
+ | /!------If something should be added, list it-------!/ | ||
+ | </ul> | ||
+ | </li> | ||
+ | <h3><a name="#Parts">Parts</a></h3> | ||
+ | <h3><a name="#Primer">Primer list</a></h3> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Primer name</th> | ||
+ | <th>Sequence</th> | ||
+ | <th>Length</th> | ||
+ | <th>Tm</th> | ||
+ | <th>GC%</th> | ||
+ | <th>Designer</th> | ||
+ | <th>Manufacturer</th> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <h3><a name="#Materials">Materials</a></h3> | ||
+ | <table> | ||
+ | <caption><a name="#Kit">Kit</caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Name</th> | ||
+ | <th>Supplier</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <table> | ||
+ | <caption><a name="#Enzyme">Restriction Enzyme</caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Name</th> | ||
+ | <th>Supplier</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | <h3><a name="#Methods">Methods</a></h3> | ||
+ | <footer> | ||
+ | <div id="footer_logos"> | ||
+ | <img src="" width="" height=""> | ||
+ | </div> | ||
+ | <div id="footer_contact"> | ||
+ | <img src="" width="" height=""> | ||
+ | </div> | ||
+ | <div id="footer_social"> | ||
+ | <a href="mailto:igemkyoto@gmail.com"><img src=""></a> | ||
+ | <a href="https://twitter.com/igemkyoto"><img src=""></a> | ||
+ | <a href="https://www.facebook.com/IgemKyoto/"><img src=""></a> | ||
+ | </div> | ||
+ | </footer> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</html> | </html> |
Revision as of 10:28, 7 September 2017
Material&Methods
- Parts
- Primer list
- Materials
- 3-1 Kit
- 3-2 Restricton Enzyme
- 3-3 Polymerase
- 3-4 DNA ligase
- 3-5 Marker
- 3-6 Organism
- 3-7 Antibiotics
- 3-8 Equipment
- 3-9 Backborns
- 3-10 Buffer
- 3-11 SDSPAGE/Western blotting /!-----If something should be added, list it from here-----!/
- Methods
- 4-1 Miniprep
- 4-2 Gel Extraction
- 4-3 Restrict Enzyme Digestion
- 4-4 Ligation
- 4-5 Transformation
- 4-6 PCR
- 4-7 Sequencing
- 4-8 RT-PCR
- 4-10 Western blotting(Membrane fraction) /!------If something should be added, list it-------!/
Parts
Primer list
Primer name | Sequence | Length | Tm | GC% | Designer | Manufacturer |
---|
Materials
Name | Supplier |
---|---|
Name | Supplier |
---|---|