Difference between revisions of "Team:Kyoto/Experiments"
| Line 48: | Line 48: | ||
<li><a href="#PCR">4-6 PCR</a></li> | <li><a href="#PCR">4-6 PCR</a></li> | ||
<li><a href="#Sequencing">4-7 Sequencing</a></li> | <li><a href="#Sequencing">4-7 Sequencing</a></li> | ||
| − | <li><a href="# | + | <li><a href="#RTPCR">4-8 RT-PCR</a></li> |
<li><a href="#Western>4-9 Basic protocol of Western blotting</a></li> | <li><a href="#Western>4-9 Basic protocol of Western blotting</a></li> | ||
<li><a href="#Western2">4-10 Western blotting(Membrane fraction)</a></li> | <li><a href="#Western2">4-10 Western blotting(Membrane fraction)</a></li> | ||
| Line 69: | Line 69: | ||
<h3><a name="#Materials">Materials</a></h3> | <h3><a name="#Materials">Materials</a></h3> | ||
<table> | <table> | ||
| − | < | + | <h4><a name="#Kit">Kit</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
| Line 84: | Line 84: | ||
</table> | </table> | ||
<table> | <table> | ||
| − | < | + | <h4><a name="#Enzyme">Restriction Enzyme</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
| Line 97: | Line 97: | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Polymerase"></a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Ligase">DNA Ligase</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Marker">Marker</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Organism"></a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Antibiotics">Antibiotics</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Equipment">Equipment</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Backbones">Backbones</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#Buffer">Buffer</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table> | ||
| + | <h4><a name="#SDSPAGE">SDSPAGE/Western blotting</a></h4> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Supplier</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
<h3><a name="#Methods">Methods</a></h3> | <h3><a name="#Methods">Methods</a></h3> | ||
| + | <h4><a name="#Miniprep">Miniprep</a></h4> | ||
| + | <h4><a name="#Gel">Gel Extraction</a></h4> | ||
| + | <h4><a name="#Digestion">Restriction Enzyme Digestion</a></h4> | ||
| + | <h4><a name="#Ligation">Ligation</a></h4> | ||
| + | <h4><a name="#Transformation">Transformation</a></h4> | ||
| + | <h4><a name="#PCR">PCR</a></h4> | ||
| + | <h4><a name="#Sequencing">Sequenicing</a></h4> | ||
| + | <h4><a name="#RTPCR">RT-PCR</a></h4> | ||
| + | <h4><a name="#Western">Basic protcol of Western blotting</a></h4> | ||
| + | <h4><a name="#western2">Western blotting(Membrane fraction)</a></h4> | ||
<footer> | <footer> | ||
<div id="footer_logos"> | <div id="footer_logos"> | ||
Revision as of 12:25, 7 September 2017
Material&Methods
- Parts
- Primer list
- Materials
- 3-1 Kit
- 3-2 Restricton Enzyme
- 3-3 Polymerase
- 3-4 DNA ligase
- 3-5 Marker
- 3-6 Organism
- 3-7 Antibiotics
- 3-8 Equipment
- 3-9 Backborns
- 3-10 Buffer
- 3-11 SDSPAGE/Western blotting /!-----If something should be added, list it from here-----!/
- Methods
- 4-1 Miniprep
- 4-2 Gel Extraction
- 4-3 Restrict Enzyme Digestion
- 4-4 Ligation
- 4-5 Transformation
- 4-6 PCR
- 4-7 Sequencing
- 4-8 RT-PCR
- 4-10 Western blotting(Membrane fraction) /!------If something should be added, list it-------!/
Parts
Primer list
| Primer name | Sequence | Length | Tm | GC% | Designer | Manufacturer |
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Materials
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