Difference between revisions of "Team:NKU China"
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<div id="header">DESCRIPTION</div> | <div id="header">DESCRIPTION</div> | ||
| − | In the process of oil recovering by water injection method, it will form large water channels in the bottom. <br/> | + | In the process of oil recovering by water injection method, it will form large water channels in the bottom. <br/><br/> |
Water mainly flows through these channels, which stops brushing the oil hidden in the minor channel branches in soil, so that the remaining oil will be trapped into the branches. </br> | Water mainly flows through these channels, which stops brushing the oil hidden in the minor channel branches in soil, so that the remaining oil will be trapped into the branches. </br> | ||
Our project is aiming to engineer Enterobacter sp. <i>FY-07</i> (which is separated from oilfield produced water and able to produce cellulose naturally), so as to controllably produce rhamnolipid and cellulose.</br> | Our project is aiming to engineer Enterobacter sp. <i>FY-07</i> (which is separated from oilfield produced water and able to produce cellulose naturally), so as to controllably produce rhamnolipid and cellulose.</br> | ||
Rhamnolipid is a biosurfactant with the ability of emulsifying oil, so the oil can be easily washed out. | Rhamnolipid is a biosurfactant with the ability of emulsifying oil, so the oil can be easily washed out. | ||
| − | Cellulose can clog large water channels, helping water enter into the gaps of soil and wash out the oil within them.</br> | + | Cellulose can clog large water channels, helping water enter into the gaps of soil and wash out the oil within them.</br><br/> |
Our project is aimed at improving oil recovery rate in the oil harvesting process using this kind of bacteria. | Our project is aimed at improving oil recovery rate in the oil harvesting process using this kind of bacteria. | ||
| − | <br/> | + | <br/><br/> |
<div class="sub-header">PROCESS ACHIEVED</div> | <div class="sub-header">PROCESS ACHIEVED</div> | ||
1. We have cloned rhamnolipid synthesis gene (<i>rhIABC</i>) in Pseudomonas aeruginosa, and transformed it into <i>Escherichia coli BL21</i> and successfully achieved the expression of <i>rhIABC</i> in <i>E.coli BL21</i>. | 1. We have cloned rhamnolipid synthesis gene (<i>rhIABC</i>) in Pseudomonas aeruginosa, and transformed it into <i>Escherichia coli BL21</i> and successfully achieved the expression of <i>rhIABC</i> in <i>E.coli BL21</i>. | ||
| − | <br/> | + | <br/><br/> |
2. We have transformed rhIABC into <i>FY-07</i> and successfully fulfilled the co-production of rhamnolipid and cellulose. | 2. We have transformed rhIABC into <i>FY-07</i> and successfully fulfilled the co-production of rhamnolipid and cellulose. | ||
| − | <br/> | + | <br/><br/> |
3. Based on <i>E.coli</i> fimbriae control system (<i>E.coli</i> fimbriae (Fim) phase variation system), we have finished the switch design, to whose both ends GFP and RFP have been linked. This modified switch has been transformed into <i>BL21</i> and proved to be feasible. | 3. Based on <i>E.coli</i> fimbriae control system (<i>E.coli</i> fimbriae (Fim) phase variation system), we have finished the switch design, to whose both ends GFP and RFP have been linked. This modified switch has been transformed into <i>BL21</i> and proved to be feasible. | ||
| − | <br/> | + | <br/><br/> |
<div class="sub-header">FUTURE PLAN</div> | <div class="sub-header">FUTURE PLAN</div> | ||
1. Finish the construction of plasmid. | 1. Finish the construction of plasmid. | ||
| − | <br/> | + | <br/><br/> |
2. Introduce molecular switch and rhamnolipid synthase into <i>BscA</i> gene knock out <i>FY-07</i> strain. | 2. Introduce molecular switch and rhamnolipid synthase into <i>BscA</i> gene knock out <i>FY-07</i> strain. | ||
| − | <br/> | + | <br/><br/> |
3. Achieve controllable expression of rhamnolipid and cellulose in <i>FY-07</i> strain. | 3. Achieve controllable expression of rhamnolipid and cellulose in <i>FY-07</i> strain. | ||
| − | <br/> | + | <br/><br/> |
4. Introduce suicide system and ensure the controllability. | 4. Introduce suicide system and ensure the controllability. | ||
| − | <br/> | + | <br/><br/> |
<div class="sub-header">COMMUNICATION AND CORPORATIONS</div> | <div class="sub-header">COMMUNICATION AND CORPORATIONS</div> | ||
1. We expect to communicate with the practitioners in oil industry so as to know more about applying petroleum microorganisms into oil production. Please contact us if you are a practitioner in oil industry who has some knowledge in this field. | 1. We expect to communicate with the practitioners in oil industry so as to know more about applying petroleum microorganisms into oil production. Please contact us if you are a practitioner in oil industry who has some knowledge in this field. | ||
| − | <br/> | + | <br/><br/> |
2. We are looking for a suitable suicide system so that the bacteria will not survive in the environment for a long time. Please contact us if you have some recommendations. | 2. We are looking for a suitable suicide system so that the bacteria will not survive in the environment for a long time. Please contact us if you have some recommendations. | ||
| − | <br/> | + | <br/><br/> |
3. Please do not hesitate to contact us if you are interested in our project. We are looking forward to communicating with you! | 3. Please do not hesitate to contact us if you are interested in our project. We are looking forward to communicating with you! | ||
| − | <br/> | + | <br/><br/> |
</div> | </div> | ||
</body> | </body> | ||
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color:#FFFFFF; | color:#FFFFFF; | ||
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#header{ | #header{ | ||
Revision as of 13:03, 12 September 2017
DESCRIPTION
In the process of oil recovering by water injection method, it will form large water channels in the bottom. Water mainly flows through these channels, which stops brushing the oil hidden in the minor channel branches in soil, so that the remaining oil will be trapped into the branches. Our project is aiming to engineer Enterobacter sp. FY-07 (which is separated from oilfield produced water and able to produce cellulose naturally), so as to controllably produce rhamnolipid and cellulose. Rhamnolipid is a biosurfactant with the ability of emulsifying oil, so the oil can be easily washed out. Cellulose can clog large water channels, helping water enter into the gaps of soil and wash out the oil within them.
Our project is aimed at improving oil recovery rate in the oil harvesting process using this kind of bacteria.
PROCESS ACHIEVED
1. We have cloned rhamnolipid synthesis gene (rhIABC) in Pseudomonas aeruginosa, and transformed it into Escherichia coli BL21 and successfully achieved the expression of rhIABC in E.coli BL21.
2. We have transformed rhIABC into FY-07 and successfully fulfilled the co-production of rhamnolipid and cellulose.
3. Based on E.coli fimbriae control system (E.coli fimbriae (Fim) phase variation system), we have finished the switch design, to whose both ends GFP and RFP have been linked. This modified switch has been transformed into BL21 and proved to be feasible.
FUTURE PLAN
1. Finish the construction of plasmid.
2. Introduce molecular switch and rhamnolipid synthase into BscA gene knock out FY-07 strain.
3. Achieve controllable expression of rhamnolipid and cellulose in FY-07 strain.
4. Introduce suicide system and ensure the controllability.
COMMUNICATION AND CORPORATIONS
1. We expect to communicate with the practitioners in oil industry so as to know more about applying petroleum microorganisms into oil production. Please contact us if you are a practitioner in oil industry who has some knowledge in this field.
2. We are looking for a suitable suicide system so that the bacteria will not survive in the environment for a long time. Please contact us if you have some recommendations.
3. Please do not hesitate to contact us if you are interested in our project. We are looking forward to communicating with you!
