Difference between revisions of "Team:Munich/Hardware"

Line 130: Line 130:
 
<tr><td colspan=2 align=right valign=center height=10>
 
<tr><td colspan=2 align=right valign=center height=10>
 
<font size=2>Date</font>
 
<font size=2>Date</font>
 +
</td></tr>
 +
<tr><td colspan=2 align=left valign=center>
 +
<font size=7 color=#444444><b>Abstract</b></font>
 +
</td></tr>
 +
<tr><td align=center valign=center>
 +
<p style="text-align:justify">
 +
<font size=3 color=#444444>
 +
The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.
 +
</font>
 +
</p>
 +
</font>
 +
</td><td align=center valign=center>
 +
<img src="placeholder.jpg" width="420">
 +
</td></tr>
 +
<tr><td align=center valign=center>
 +
<p style="text-align:justify">
 +
<font size=3 color=#444444>
 +
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
 +
</font>
 +
</p>
 +
</td><td align=center valign=center>
 +
<p style="text-align:justify">
 +
<font size=3 color=#444444>
 +
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 +
</font>
 +
</p>
 
</td></tr>
 
</td></tr>
 
<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
 
<tr><td class="no-padding" colspan=2 align=right valign=center height=10>

Revision as of 14:31, 14 September 2017


Date
Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.

Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.