Revision as of 11:32, 18 September 2017

Material&Methods

1. Parts
2. Primer list
3. Materials
• 3-1 Kit
• 3-2 Restricton Enzyme
• 3-3 Polymerase
• 3-4 DNA ligase
• 3-5 Marker
• 3-6 Organism
• 3-7 Antibiotics
• 3-8 Equipment
• 3-9 Backborns
• 3-10 Buffer
• 3-11 SDSPAGE/Western blotting
4. Methods
• 4-1 Miniprep
• 4-2 Gel Extraction
• 4-3 Restrict Enzyme Digestion
• 4-4 Ligation
• 4-5 Transformation
• 4-6 PCR
• 4-7 Sequencing
• 4-8 RT-PCR
• 4-10 Western blotting(Membrane fraction)
/!------If something should be added, list it-------!/

Parts

Primer list

Primer name	Sequence	Length	Tm	GC%	Designer	Manufacturer

Materials

Kit

Name	Supplier

Restriction Enzyme

Name	Supplier

Name	Supplier

DNA Ligase

Name	Supplier

Marker

Name	Supplier

Name	Supplier

Antibiotics

Name	Supplier

Equipment

Name	Supplier

Backbones

Name	Supplier

Buffer

Name	Supplier

SDSPAGE/Western blotting

Name	Supplier

Methods

Miniprep

Gel Extraction

Restriction Enzyme Digestion

Ligation

Transformation

PCR

Sequenicing

RT-PCR

Basic protcol of Western blotting

Western blotting(Membrane fraction)