Difference between revisions of "Team:Kyoto/Experiments"
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<h3><a name="#Parts">Parts</a></h3> | <h3><a name="#Parts">Parts</a></h3> | ||
<h3><a name="#Primer">Primer list</a></h3> | <h3><a name="#Primer">Primer list</a></h3> | ||
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<th>Primer name</th> | <th>Primer name</th> | ||
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<h3><a name="#Materials">Materials</a></h3> | <h3><a name="#Materials">Materials</a></h3> | ||
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<h5><a name="#Kit">Kit</a></h5> | <h5><a name="#Kit">Kit</a></h5> | ||
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<h4><a name="#Enzyme">Restriction Enzyme</a></h4> | <h4><a name="#Enzyme">Restriction Enzyme</a></h4> | ||
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<h4><a name="#Polymerase"></a></h4> | <h4><a name="#Polymerase"></a></h4> | ||
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<h4><a name="#Ligase">DNA Ligase</a></h4> | <h4><a name="#Ligase">DNA Ligase</a></h4> | ||
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<h4><a name="#Marker">Marker</a></h4> | <h4><a name="#Marker">Marker</a></h4> | ||
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<h4><a name="#Organism"></a></h4> | <h4><a name="#Organism"></a></h4> | ||
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<h4><a name="#Antibiotics">Antibiotics</a></h4> | <h4><a name="#Antibiotics">Antibiotics</a></h4> | ||
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<h4><a name="#Equipment">Equipment</a></h4> | <h4><a name="#Equipment">Equipment</a></h4> | ||
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<h4><a name="#Backbones">Backbones</a></h4> | <h4><a name="#Backbones">Backbones</a></h4> | ||
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<h4><a name="#Buffer">Buffer</a></h4> | <h4><a name="#Buffer">Buffer</a></h4> | ||
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<h4><a name="#SDSPAGE">SDSPAGE/Western blotting</a></h4> | <h4><a name="#SDSPAGE">SDSPAGE/Western blotting</a></h4> | ||
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Revision as of 11:42, 18 September 2017
Material&Methods
- Parts
- Primer list
- Materials
- 3-1 Kit
- 3-2 Restricton Enzyme
- 3-3 Polymerase
- 3-4 DNA ligase
- 3-5 Marker
- 3-6 Organism
- 3-7 Antibiotics
- 3-8 Equipment
- 3-9 Backborns
- 3-10 Buffer
- 3-11 SDSPAGE/Western blotting
- Methods
- 4-1 Miniprep
- 4-2 Gel Extraction
- 4-3 Restrict Enzyme Digestion
- 4-4 Ligation
- 4-5 Transformation
- 4-6 PCR
- 4-7 Sequencing
- 4-8 RT-PCR
- 4-10 Western blotting(Membrane fraction) /!------If something should be added, list it-------!/
Parts
Primer list
| Primer name | Sequence | Length | Tm | GC% | Designer | Manufacturer |
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Materials
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