Difference between revisions of "Team:Aix-Marseille/Parts"

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==Basic Parts==
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During the summer, several basic parts were constructed.
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'''We create basic part for any of our approach : '''
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*Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - BBa_K2255004
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*Multi-Tag (Rfc25) - BBa_K2255003
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'''We design 3 parts for the quorum sensing approach :'''
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*Enoyl-CoA hydratase (Rfc10) - BBa_K2255000
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*Acyl-CoA isomerase  (Rfc10) - BBa_K2255001
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*Thioesterase (Rfc10) - BBa_K2255002
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'''We design a part for the disruption of the biofilm :'''
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*EPS-Depolymerase (Rfc25) - BBa_K2255006
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'''Finally, we design some basic part for the phage-like particle approach :'''
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*Domain 3 of p3 from M13 (Rfc25) - BBa_K2255005
 +
*Signal sequence of p3 from M13 (Rfc25) - BBa_K2255007
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The collection part use for the creation of phage-like particle, those part correspond to the domaine 1 and 2 of the p3 protein. They allow the phage-like particle to target the specific bacterium. To test the specificity of our phage-like particle and to target other pathogenic bacterium we design a large scale of attachment protein :
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*p3_E.coli (Rfc25) - BBa_K2255008
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*p3_ N.gonorrhoeae (Rfc25) - BBa_K2255009
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*p3_P.aeruginosa (Rfc25) - BBa_K2255010
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*p3_R.solanacearum_RSM1 (Rfc25) - BBa_K2255011
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*p3_R.solanacearum_RSS1 (Rfc25) - BBa_K2255012
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*p3_V.Cholerae_CTXΦ (Rfc25) - BBa_K2255013
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*p3_V.Cholerae_fs2 (Rfc25) - BBa_K2255014
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*p3_V.Cholerea_VGJΦ (Rfc25) - BBa_K2255015
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*p3_X.campestris (Rfc25) - BBa_K2255016
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*p3_X.fastidiosa (Rfc25) - BBa_K2255017
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*p3_X.fuscans (Rfc25) - BBa_K2255018

Revision as of 13:53, 18 September 2017

Parts

Contents

Basic Parts

During the summer, several basic parts were constructed.

We create basic part for any of our approach :

  • Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - BBa_K2255004
  • Multi-Tag (Rfc25) - BBa_K2255003

We design 3 parts for the quorum sensing approach :

  • Enoyl-CoA hydratase (Rfc10) - BBa_K2255000
  • Acyl-CoA isomerase (Rfc10) - BBa_K2255001
  • Thioesterase (Rfc10) - BBa_K2255002

We design a part for the disruption of the biofilm :

  • EPS-Depolymerase (Rfc25) - BBa_K2255006

Finally, we design some basic part for the phage-like particle approach :

  • Domain 3 of p3 from M13 (Rfc25) - BBa_K2255005
  • Signal sequence of p3 from M13 (Rfc25) - BBa_K2255007

The collection part use for the creation of phage-like particle, those part correspond to the domaine 1 and 2 of the p3 protein. They allow the phage-like particle to target the specific bacterium. To test the specificity of our phage-like particle and to target other pathogenic bacterium we design a large scale of attachment protein :

  • p3_E.coli (Rfc25) - BBa_K2255008
  • p3_ N.gonorrhoeae (Rfc25) - BBa_K2255009
  • p3_P.aeruginosa (Rfc25) - BBa_K2255010
  • p3_R.solanacearum_RSM1 (Rfc25) - BBa_K2255011
  • p3_R.solanacearum_RSS1 (Rfc25) - BBa_K2255012
  • p3_V.Cholerae_CTXΦ (Rfc25) - BBa_K2255013
  • p3_V.Cholerae_fs2 (Rfc25) - BBa_K2255014
  • p3_V.Cholerea_VGJΦ (Rfc25) - BBa_K2255015
  • p3_X.campestris (Rfc25) - BBa_K2255016
  • p3_X.fastidiosa (Rfc25) - BBa_K2255017
  • p3_X.fuscans (Rfc25) - BBa_K2255018