Difference between revisions of "Team:INSA-UPS France/test/description"

Line 3: Line 3:
 
{{INSA-UPS_France/Header_new}}
 
{{INSA-UPS_France/Header_new}}
  
 +
<html>
  
<html>
 
 
<style>
 
<style>
  
main{
 
  position:relative;
 
  overflow: auto;
 
  height:100%;
 
}
 
  
 
.main_content{
 
.main_content{
Line 38: Line 33:
 
section{
 
section{
 
   background-color: rgba(255,255,255,0.2);
 
   background-color: rgba(255,255,255,0.2);
  padding:3% 10%;
 
 
   text-align: justify;
 
   text-align: justify;
 
   border-radius:20px;
 
   border-radius:20px;
Line 70: Line 64:
 
   position:absolute;
 
   position:absolute;
 
   right:0;
 
   right:0;
   width:400px;
+
   width:800px;
 
   bottom:-25px;
 
   bottom:-25px;
 
}
 
}
/*for now*/
+
 
sup{
+
 
  display: none;
+
}
+
 
</style>
 
</style>
  
 
<!-- C O N T E N T -->
 
<!-- C O N T E N T -->
 
    
 
    
 +
<div class="site">
 +
  <header class="site-header js-sticky is-sticky">
 +
 +
    <div class="container" style="position:relative; ">
 +
    <a href="#" class="site-header__title">
 +
      <img src="logo.png" alt="" style="width:70px;">
 +
    </a>
 +
    <div class="site-header__title">
 +
    Croc'n Cholera <br/>
 +
    <span style="font-size:12pt;">iGEM UPS-INSA Toulouse 2017</span>
 +
    </div>
 +
 +
    <nav class="site-menu" style="text-align: center;">
 +
     
 +
      <div class="site-menu__item no-subnav">
 +
        <a href="index3.html"><i class="fa fa-home"></i></a>
 +
      </div>
 +
      <div class="site-menu__item">
 +
        <div class="cat_name">Team <i class="fa fa-caret-down"></i></div>
 +
        <ul class="subnav">
 +
          <li><a href="members.html">Members</a></li>
 +
          <li><a href="supports.html">Supports</a></li>
 +
          <li><a href="attributions.html">Attributions</a></li>
 +
          <li><a href="funfacts.html">Fun facts</a></li>
 +
          <li><a href="collaborations.html">Collaborations</a></li>
 +
        </ul>
 +
      </div>
 +
      <div class="site-menu__item">
 +
        <div class="cat_name">Project <i class="fa fa-caret-down"></i></div>
 +
        <ul class="subnav">
 +
          <li><a href="description.html">Description</a></li>
 +
          <li><a href="design.html">Design</a></li>
 +
          <li><a href="results.html">Results</a></li>
 +
          <li><a href="parts.html">Parts</a></li>
 +
          <li><a href="entrepreneurship.html">Entrepreneurship</a></li>
 +
        </ul>
 +
      </div>
 +
      <div class="site-menu__item">
 +
        <div class="cat_name">Model <i class="fa fa-caret-down"></i></div>
 +
        <ul class="subnav">
 +
          <li><a href="modeloverview.html">Overview</a></li>
 +
          <li><a href="sbgnrepresentation.html">SBGN Representation</a></li>
 +
          <li><a href="odesystem.html">ODE System</a></li>
 +
          <li><a href="modelresults.html">Results</a></li>
 +
        </ul>
 +
      </div>
 +
      <div class="site-menu__item">
 +
        <div class="cat_name">Notebook <i class="fa fa-caret-down"></i></div>
 +
        <ul class="subnav">
 +
          <li><a href="labbook.html">Lab Book</a></li>
 +
          <li><a href="experiments.html">Experiments</a></li>
 +
          <li><a href="safety.html">Safety</a></li>
 +
        </ul>
 +
      </div>
 +
      <div class="site-menu__item">
 +
        <div class="cat_name">Outreach <i class="fa fa-caret-down"></i></div>
 +
        <ul class="subnav">
 +
          <li><a href="hpoverview.html">Overview</a></li>
 +
          <li><a href="publicengagement.html">Public Engagements</a></li>
 +
          <li><a href="hpsilver.html">Silver</a></li>
 +
          <li><a href="ihp">Integrated Human Practices</a></li>
 +
          <li><a href="press.html">Press</a></li>
 +
        </ul>
 +
      </div>
 +
      <div class="site-menu__item no-subnav">
 +
        <a href="achievements.html"><i class="fa fa-trophy"></i></a>
 +
      </div>
 +
 +
      </nav>
 +
    </div>
 +
  </header>
 +
 +
 
 +
 +
 
   <main class="site-main">
 
   <main class="site-main">
 
   <div class="main_content">
 
   <div class="main_content">
Line 89: Line 156:
 
     <div class="main_title">
 
     <div class="main_title">
 
       <div>
 
       <div>
         <p>Description</p>
+
         <p>Supports</p>
 
       </div>
 
       </div>
       <img src="https://static.igem.org/mediawiki/2017/e/e8/T--INSA-UPS_France--description_croco.png" alt="">
+
       <img src="https://static.igem.org/mediawiki/2017/6/60/T--INSA-UPS_France--Sponsors_thank_you.png" alt="">
 
     </div>
 
     </div>
  
 
     <style>
 
     <style>
    /* ASIDE NAV */
+
       .sponsor{
       .left_container{
+
         width:100%;
         width:25%;
+
        position:absolute;
+
        top:400px;
+
        padding:0;
+
        display:inline-block;
+
        font-family: 'Quicksand', sans-serif;
+
        font-weight: 400;
+
        font-size:16pt;
+
        text-align: right;
+
 
       }
 
       }
 
+
       .sponsor td{
       .aside-nav__item + .aside-nav__item{
+
         text-align: center;
         margin-top:20px;
+
 
       }
 
       }
       .aside-nav__item{
+
       .sponsor p{
         width:100%;
+
         text-align: justify;
         position:relative;
+
         font-size:1.2vw;
 +
        font-family: "Merriweather", serif;
 +
        font-weight: 300;
 
       }
 
       }
       .aside-nav__item:after{
+
       .sponsor a{
         content:'';
+
        text-decoration: none;
         border:solid #3377a8 3px;
+
        color:black;
 +
      }
 +
      .section-left-70{
 +
         width:65%;
 +
        background: rgba(255,255,255,0.2);
 +
         border-radius: 20px;
 +
        padding:20px;   
 +
      }
 +
      .sponsor h1{
 
         position:absolute;
 
         position:absolute;
         right:-30px;
+
         top:-20px;
         top:40%;
+
         font-family: "Quicksand", sans-serif;  
        width:12px;
+
         font-size:2vw;              
        height:12px;
+
        border-radius: 12px;  
+
         margin-left:20px;  
+
 
       }
 
       }
       .aside-nav__item.selected-item:after{
+
       .sponsor h2{
         background:#3377a8;
+
        text-align: center;
 +
        font-family: "Quicksand", sans-serif;
 +
         font-size:2vw;
 +
        margin-bottom:30px;
 
       }
 
       }
       .aside-fixed{
+
 
         position:fixed;
+
       .img-right-30{
         top:100px;
+
         width:30% !important;
        width:22.5%;
+
         vertical-align: middle;
 
       }
 
       }
       .aside-nav__item a, .aside-nav__item a:focus, .aside-nav__item a:visited, .aside-nav__item a:hover{
+
       .logo-sponsor{
         text-decoration: none;
+
         width:300px;
         color:black;
+
         margin-bottom:30px;
 
       }
 
       }
       .aside-nav__item a:hover{
+
     
         font-weight: 700;
+
       .photo-sponsor{
 +
         width:100%;
 
       }
 
       }
 
     </style>
 
     </style>
 
+
     <table class="sponsor" style="border-spacing:20px;">
     <div class="left_container">
+
       <tr>
    <div class="left_container__inside">
+
        <td class="section-100" colspan="2">         
       <div class="aside-nav__item">
+
            <h1>Our institutions</h1>
      <a href="#a1" data-number="1">
+
            <a href="http://www.univ-tlse3.fr/home/home-page-en-379161.kjsp?RH=ACCUEIL"><img src="https://static.igem.org/mediawiki/2017/2/28/T--INSA-UPS_France--Logo_ups.png" class="logo-sponsor" alt=""></a>
         Combine to improve: a synthetic biology scale-up
+
            <p>
      </a>
+
              With its roots dating back to the former University of Toulouse, founded in 1229, <b>Toulouse III &ndash; Paul Sabatier University </b>was officially created in 1969 by merging the former Faculties of Medecine, Pharmacy and Science. Today, the Toulouse III - Paul Sabatier University is one of the leading universities in France, renowned for its scientific influence, the diversity of its laboratories, and the courses it offers in the fields of science, health, sport, technology and engineering.
      </div>
+
            </p>        
      <div class="aside-nav__item">
+
         </td>
      <a href="#a2" data-number="2">
+
       </tr>
        A core: prokaryotic eukaryotic communication
+
       <tr>
      </a>
+
        <td class="section-100" colspan="2">
      </div>
+
            <a href="http://www.insa-toulouse.fr/en/index.html"><img src="https://static.igem.org/mediawiki/2017/2/2d/T--INSA-UPS_France--Logo_insatoulouse.png" alt="" class="logo-sponsor"></a>
      <div class="aside-nav__item">
+
            <p>
      <a href="#a3" data-number="3">
+
              With 14,000 alumni, <b>INSA Toulouse</b> is an international, multidisciplinary, state engineering school recognized for the excellence of its five-year curriculum. Created in 1963, it has made a priority out of developing its international dimension as INSA Toulouse has worked to attract many foreign students and developed international programs. Aside from training engineers, its mission includes developing ongoing training, scientific research as well as its valorization, which is why it offers research masters and PhDs.
         A microbial consortium against cholera
+
            </p>        
      </a>
+
         </td>
       </div>
+
       </tr>
       <div class="aside-nav__item">
+
       <tr>
      <a href="#a4" data-number="4">
+
        <td class="section-100" colspan="2">
        Finding a sensor
+
          <a href="https://www.insa-lyon.fr/en"><img src="https://static.igem.org/mediawiki/2017/1/18/T--INSA-UPS_France--Logo_insalyon.png" alt="" class="logo-sponsor"></a>
      </a>
+
          <p>
      </div>
+
            <b>INSA Lyon</b> is a leading engineering school with strong humanist values that have formed the basis of its business model for more than 50 years. Created in 1957 by philosopher Gaston Berger and Rector Jean Capelle, <b>INSA Lyon</b> is the oldest and largest of the six schools making up the INSA Group. INSA engineers lead careers built around a logic of high adaptability, a pioneering approach to science and technology, and a strong sense of social responsibility. This hallmark of the INSA Lyon model is a living tribute to its two founders. It is to them that we owe the humanist commitment of the INSA schools, and their openness to different origins, cultures and social backgrounds.
      <div class="aside-nav__item">
+
          </p>
      <a href="#a5" data-number="5">
+
        </td>
         Finding an effector
+
      </tr>
      </a>
+
     </table>
       </div>
+
       <div class="aside-nav__item">
+
      <a href="#a6" data-number="6">
+
        Our system
+
      </a>
+
      </div>
+
    </div>
+
     </div>
+
  
 
     <style>
 
     <style>
    section img{
+
      .section-100{
      width:100%;
+
        width:100%;
    }
+
        background: rgba(255,255,255,0.2);
    section h1{
+
        border-radius: 20px;
      font-family: 'Quicksand', sans-serif;
+
        padding:20px;
      font-size:34pt;
+
        text-align: justify;
      margin-top:-60px;
+
        position:relative;
      text-align: right;
+
       
    }
+
       }
    section p{
+
      .sponsor-name{
       font-family: 'Merriweather', serif;
+
        width:30%;
      font-size:14pt;
+
        display: inline-block;
      font-weight: 300;
+
        text-align: center;
       margin-top:20px;
+
       }
    }
+
      .sponsor-name img{
    .right_container{
+
        width:150px;
         width:70%;
+
      }
        margin-left:30%;
+
      @media screen and (max-width: 1200px){
    }
+
         .sponsor td{
    .article_offset{
+
          display:block;
      margin-bottom:20px;
+
          width:100%;
       border: solid 1px transparent;
+
          margin:0 auto;
    }
+
        }
 +
        .img-right-30 img{
 +
          display:none;
 +
        }
 +
       }
 
     </style>
 
     </style>
 
 
   
 
    <div class="right_container">
 
  
    <div class="article_offset" id="a1"></div>
+
    <table class="sponsor" style="border-spacing:20px;">
    <section>
+
        <tr>
      <h1>Combine to improve: a synthetic biology scale-up</h1>
+
          <td class="section-left-70">
      <p>
+
            <h1>Our sponsors</h1>
        Nature offers us a wide variety of tools in order to sense precise chemical or physical parameters. For example, enzymes and receptors are specialized for unique or restrained range of molecule, or even for specific light wavelength.<sup>1,2,3</sup> Obviously, as mankind progressed in the comprehension of life science, he wanted to take advantage from this extreme diversity and specialization. Synthetic biology was born thanks to those expectations in the early of the twentieth century<sup>4</sup> and made quite notable breakthrough. However, most of those works were based on the insertion of a lot of DNA information on one single model, typically <i>E. coli</i>. We observed that all those informations are not always compatible with the use of a unique microbial chassis : although standard microbial models are quite modular, they have a particular membrane, specific pathways and different ways to do protein maturation.
+
            <a href="http://www.lisbp.fr/en/index.html"><img src="https://static.igem.org/mediawiki/2017/3/3e/T--INSA-UPS_France--Logo_lisbp.png"  class="logo-sponsor" alt="" style="width:200px !important;"></a>
      </p>
+
            <h2><a href="http://www.lisbp.fr/en/index.html">LISBP (Laboratoire d'Ing&eacute;nierie des Syst&egrave;mes Biologique et des Proc&eacute;d&eacute;s)</a></h2>          
      <p>
+
            <p>
        Why should we persist to use a single model when we have access to a wide diversity of organisms?
+
              <b>LISBP</b> is multidisciplinary (microbiology, biocatalysis, bioprocesses, separation techniques&hellip;); it works on uniting Systems Biology, Processes and Engineering, and has a triple engagement centred around Research, Training, and Transfer and Exploitation. With the ambitious aim of federating competence &ldquo;from gene to process&rdquo; while combining scientific excellence with economic and social pertinence, LISPB is crossing divisions, applying a multi scale strategy, and building networks in the local and international environment.
      </p>
+
            </p>
      <p>
+
          </td>
        For our iGEM project, we decided to focus on the multi organisms aspect, making communication between prokaryotic and eukaryotic possible.
+
          <td class="img-right-30">
      </p>
+
            <img src="https://static.igem.org/mediawiki/2017/2/23/T--INSA-UPS_France--Sponsors_photo_LISBP.jpg" class="photo-sponsor" alt="">
    </section>
+
          </td>
 +
        </tr>
 +
        <tr>
 +
          <td class="section-left-70">
 +
            <a href="http://www.toulouse-white-biotechnology.com/en/"><img src="https://static.igem.org/mediawiki/2017/5/56/T--INSA-UPS_France--Logo_twb.png" class="logo-sponsor" alt="" style="width:200px !important;"></a>
 +
            <h2><a href="http://www.toulouse-white-biotechnology.com/en/">Toulouse White Biotechnology</a></h2>
 +
            <p>
 +
              <b>Toulouse White Biotechnology (TWB)</b> is a preindustrial demonstrator whose goal is to speed up the development of industrial biotechnology by facilitating exchanges between public research and industry. It contributes to the expansion of a bioeconomy based on the use of renewable carbon in the fields of chemistry, materials and energy.
 +
            </p>
 +
          </td>
 +
          <td class="img-right-30">
 +
            <img src="https://static.igem.org/mediawiki/2017/d/d8/T--INSA-UPS_France--Sponsors_photo_TWB.jpg" class="photo-sponsor" alt="">
 +
          </td>
 +
        </tr>
 +
       
 +
        <tr>
 +
          <td colspan="2" class="section-100">
 +
            <div class="sponsor-name">
 +
              <a href="http://fondation.insa-lyon.fr/"><img src="https://static.igem.org/mediawiki/2017/6/6d/T--INSA-UPS_France--Logo_fondationinsal.png" class="logo-sponsor" alt=""></a>
 +
            </div>
 +
            <div class="sponsor-name">
 +
              <a href="https://fr-fr.facebook.com/insaadnconceptclub/"><img src="https://static.igem.org/mediawiki/2017/b/b7/T--INSA-UPS_France--Logo_ia2c.png" class="logo-sponsor" alt=""></a>
 +
            </div>
 +
            <div class="sponsor-name">
 +
              <a href="https://fr-fr.facebook.com/insaadnconceptclub/http://www.biomerieux.com/en"><img src="https://static.igem.org/mediawiki/2017/8/83/T--INSA-UPS_France--Logo_biomerieux.png" class="logo-sponsor" alt=""></a>
 +
            </div>
 +
            <p>
 +
              By creating its partnership foundation in 2009, with the support of France's largest companies, <b>INSA Lyon</b> demonstrates the quality of relations it has developed over the years with its economic partners, and positions itself clearly in a strategy of corporate cooperation. From 2010, the INSA Lyon foundation and one of his main economic partners, the Biomerieux compagny, allows us to give life to our projects and to place us among the best competitors on the international scene of synthetic biology. So it is together again with theses unshakeable partners that we hope to reach the top of the podium in 2017.
 +
            </p>
 +
          </td>
 +
        </tr>
  
   
+
        <tr>
    <div class="article_offset" id="a2"></div>
+
          <td class="section-left-70">
    <section>
+
            <a href="http://www.sunwaterlife.com/en/"><img src="https://static.igem.org/mediawiki/2017/0/09/T--INSA-UPS_France--Logo_sunwaterlife.png"  class="logo-sponsor" alt="" style="width:350px !important;"></a>
      <h1>A core: prokaryotic-eukaryotic communication
+
            <p>
</h1>
+
              <b>Sunwaterlife</b> is a French start-up located in Toulouse, designer of an innovative water purification system. From a contaminated or salted water source, its sun power supplied system can produce up to 3000 liters of drinking water per day. With the size of a suitcase, it is an eco-friendly self-sustained solution, able to provide drinking water for schools, clinics, villages or in case of sanitary emergency in developing countries.
      <p>
+
            </p>
        Our first challenge was to design an eukaryote-prokaryote communication system, so we can have a standardized way of communication, with modulable input and output. This system answers to some of the synthetic biology current challenges<sup>8</sup> that are: device modularization and standardisation with the core, using the diversity of living systems and so, using the right cellular chassis, with the right genetics elements.
+
          </td>
      </p>
+
          <td class="img-right-30">
      <img src="https://static.igem.org/mediawiki/2017/b/b3/T--INSA-UPS_France--description_sense-effect.png" alt="">
+
            <img src="https://static.igem.org/mediawiki/2017/3/30/T--INSA-UPS_France--Sponsors_photo_SWL.jpg" class="photo-sponsor" alt="">
      <p class="left-p">
+
          </td>
        Team SCUT<sup>26</sup> already described a binding-receptor system built on <i>Saccharomyces cerevisiae</i><sup>18</sup>, based on the Odr-10 receptor, a G Protein Coupled Receptor isolated from Caenorhabditis elegans<sup>19</sup>. The pathway is engaged by the activation of Odr-10 and has been shown to start the mating cascade which ends with  the activation of the pFUS1 promoter by Ste12<sup>20</sup>. After a discussion with Diethard Mattanovich , the supervisor of iGEM Team Boku Vienna, it appears from his transcriptomic data that the mating pathway including Ste12 was also present on Pichia pastoris, a yeast gaining more and more interest for being a robust tool for protein and metabolite production.<sup>33</sup> Once we found a molecule factory, we needed to find a way to induce it, whenever the bacteria sensor we were using. Checking the metabolism of diacetyl on KEGG Pathway, we identified that a simple enzyme, the acetolactate synthase (ALS), processes to produce the diacetyl from pyruvate, a ubiquitous metabolite.
+
        </tr>
      </p>
+
      <img class="right-img" src="https://static.igem.org/mediawiki/2017/2/24/T--INSA-UPS_France--Description-communicate.png" alt="">
+
      <img class="left-img" src="https://static.igem.org/mediawiki/2017/9/91/T--INSA-UPS_France--description_diacetyl.png" alt="">
+
      <p class="right-p">
+
        As this mechanism relies on diacetyl detection for pFUS1 activation, we needed  the sensing module to produce this molecule. Online tools predict that the missing enzyme to catalyse the production of this molecule is the acetolactate synthase (ALS). Indeed, this enzyme catalyses acetolactate production, which is then oxidized into diacetyl. Thus we decided to add the gene responsible for ALS production in the genetic construction we put in the sensing module.
+
      </p>
+
    </section>
+
  
 +
        <tr>
 +
          <td colspan="2" class="section-100">
 +
            <a href=""><img src="https://static.igem.org/mediawiki/2017/a/ab/T--INSA-UPS_France--Logo_fsi.png"  class="logo-sponsor" alt="" style="width:350px !important;"></a>
 +
            <p>
 +
              <b>The Science and Engineering Faculty (Facult&eacute; de Sciences et d'Ing&eacute;nierie, FSI)</b> is a Training and Research Unit of Paul Sabatier University. Composed of eight academic departments, an engineering school (Upssitech) and a department devoted to teaching methods for science (IRES), the FSI is supported by more than sixty research laboratories of international dimension. Through its teaching and laboratories, as well as outreach partnerships with local industries, the FSI offers training that integrates the most recent scientific and technological advances.
 +
            </p>
 +
          </td>
 +
        </tr>
 +
       
 +
        <tr>
 +
          <td colspan="2" class="section-100">
 +
            <a href="https://www.kbs-frb.be/"><img src="https://static.igem.org/mediawiki/2017/c/cf/T--INSA-UPS_France--Logo_ernestsolvay.png"  class="logo-sponsor" alt="" style="width:350px !important;"></a>
 +
            <p>
 +
              Project achieved with the support of the <b>Ernest Solvay Fund</b>, managed by the <b>King Baudouin Foundation</b>.
 +
            </p>
 +
          </td>
 +
        </tr>
  
    <div class="article_offset" id="a3"></div>
+
        <tr>
    <section>
+
          <td class="section-left-70">
      <h1>A microbial consortium against cholera</h1>
+
            <a href="http://www.insa-toulouse.fr/fr/institution/fondation-insa-toulouse.html#"><img src="https://static.igem.org/mediawiki/2017/6/62/T--INSA-UPS_France--Logo_fondationinsat.png"  class="logo-sponsor" alt="" style="width:200px !important;"></a>
      <p>
+
            <p>
        Recently, research teams started to focus on synthetic biology in order to find a way to deal with Vibrio cholerae<sup>5,6</sup>. Additionally, some iGEM teams also took the challenge of detecting <i>V. cholerae</i>, using <i>E. coli</i> as a host for the quorum sensing system, but it seems that this strategy did not succeed7 or showed mixed results<sup>8</sup>. Most of the iGEM teams intended to prevent the cholera infection thanks to gene targeting such as cleaving toxicity genes<sup>9</sup> or inhibiting specific genes on the pathogen<sup>10</sup>. With this lack of successful stories in iGEM on mono-microorganism and the need to have a whole specialised lab in order to build a complex system of synthetic biology, this statement could be perfect to test our core in real conditions and with a useful application.  
+
              <b>INSA</b> chose to create its foundation, with Hubert Reeves renowned astrophysician as its patron, to assert its development policy and strengthenits cooperation with its socio-economic environment.One of the Foundation&rsquo;s first actions was to provide scholarships based on social criteria and commitment to studies, complementing the support already provided in the school. It also funds measures promoting accessibility on campus fordisabled students.
      </p>
+
            </p>
    </section>
+
          </td>
 +
          <td class="img-right-30">
 +
            <img src="https://static.igem.org/mediawiki/2017/7/71/T--INSA-UPS_France--Sponsors_photo_fondation_insat.jpg" class="photo-sponsor" alt="">
 +
          </td>
 +
        </tr>
  
    <div class="article_offset" id="a4"></div>
+
        <tr>
    <section>
+
          <td class="section-100" colspan="2">
      <h1>Finding a sensor</h1>
+
            <a href="http://www.adisseo.com/en/"><img src="https://static.igem.org/mediawiki/2017/9/96/T--INSA-UPS_France--Logo_adisseo.png"  class="logo-sponsor" alt=""></a>
      <p class="left-p">
+
            <p>
        First we had to detect the initial state of the system in order to activate the core. An interesting property of <i>Vibrio cholerae</i> is its quorum sensing system that depends on the CAI-1 molecule. CAI-1 directly reflects the amount of <i>V. cholerae</i> on water, and thus its virulence. Once CAI-1 is at high concentration, it can be detected and then  provides us a good way of detecting <i>V. cholerae</i> once it becomes pathogenic<sup>19</sup>. <i>Vibrio harveyi</i>, a non-pathogenic strain of <i>Vibrio</i>, showed itself as a perfect sensor of the CAI-1 molecule and thus of the quantity of <i>V. cholerae</i>. This strain fulfilled the requirements we wanted as a good synthetic biology chassis: easy to engineer, already has part of our final system such as the CqsS/CAI-1 pathway<sup>19,20</sup>. We only had to mutate the receptor and integrate the als gene to trigger diacetyl production in presence of CAI-1.
+
              <b>Adisseo</b> is a global leader in animal nutrition with more than 2,500 clients. Their mission is to provide products and services for animal feed additives with the best guarantee of safety for people and the environment.
 
+
            </p>
      </p>
+
          </td>
      <figure>
+
        </tr>
        <img src="https://static.igem.org/mediawiki/2017/e/eb/T--INSA-UPS_France--Description-sense-quorum_2.png" alt="" class="right-img">
+
        <tr>
        <figcaption><i>Quorum sensing mechanism in</i> V. cholerae</figcaption>
+
          <td class="section-100" colspan="2">
      </figure>
+
            <a href="https://www.veolia.com/en"><img src="https://static.igem.org/mediawiki/2017/9/91/T--INSA-UPS_France--Logo_veolia.png" class="logo-sponsor" alt=""></a>
      <p>
+
            <p>
        For safety reasons, we were not able to manipulate <i>V. cholerae</i> in our lab. This is why we had to engineer <i>E. coli</i> to mimick <i>V. cholerae</i> in order to further test our system. The idea was to make <i>E. coli</i> produce the specific quorum sensing molecules of <i>V. cholerae</i>: CAI-1.
+
              With over 163,000 employees worldwide, <b>Veolia</b> group designs and provides water, waste and energy management solutions that contribute to the sustainable development of communities and industries. Through its three complementary business activities, Veolia helps to develop access to resources, preserve available resources, and to replenish them.
      </p>
+
            </p>          
    </section>
+
          </td>
 
+
        </tr>
    <div class="article_offset" id="a5"></div>
+
        <tr>
    <section>
+
          <td class="section-100" colspan="2">
      <h1>Finding an effector</h1>
+
            <a href="https://www.france-science.org/-Homepage-English-.html"><img src="https://static.igem.org/mediawiki/2017/1/1a/T--INSA-UPS_France--Logo_ambassade.jpg" alt="" class="logo-sponsor" style="width:200px !important;"></a>
      <p>
+
            <h2><a href="https://www.france-science.org/-Homepage-English-.html">Office for Science &amp; Technology at the Embassy of France in the United States</a></h2>
        Because of our will to kill gram-negative bacteria, we assessed that a prokaryotic chassis could not be adequate to achieve the desired effect without drawbacks. So the information of <i>V. cholerae</i> detection must be transmitted to an eukaryotic protein expressor : <i>Pichia pastoris</i>. Once we had both the sensor, and the core, we had to act on the system. In our application, we aim to reduce the amount of <i>V. cholerae</i>, or even better, to totally kill it. We found the solution thanks to an unorthogonal approach: we looked in the environment of <i>V. cholerae</i> in order to find out a competitor, or an organism not affected by it. It appears quite fast that crocodiles have an impressive immune system, that allows them to resist against lots of  diseases vectors infections<sup>22,23,24</sup>. We used from their amazing immune system promising molecules: antimicrobial peptides that have proven to have a good killing efficiency against <i>V. cholerae</i><sup>13,14</sup>. Moreover, this effector, with a good efficiency against bacteria and the need to be secreted, was a perfect effector to implement in our multi organisms system with specialised tasks.
+
            <p>
 
+
              The <b>Office for Science and Technology (OST)</b>, a team of 24 staff members including professors, senior researchers and engineers located in the Embassy (Washington, DC) and 6 consular offices (Atlanta - Boston - Chicago - Houston - Los Angeles - San Francisco) is dedicated to bilateral FR-US collaborations in Science and Technology.
      </p>
+
            </p>          
      <p>
+
          </td>
         Those peptides are cationic molecules and can target bacterium membranes, to create pores in it, leading to the lysis of the cells<sup>24</sup>.
+
         </tr>
      </p>
+
        <tr>
      <img src="https://static.igem.org/mediawiki/2017/8/80/T--INSA-UPS_France--Description-kill.png" alt="">
+
          <td class="section-100" colspan="2">          
      <p class="left-p">
+
          <a href="http://www.univ-tlse3.fr/associations-+/do-you-have-a-project--378066.kjsp?RH=1238417866394"> <img src="https://static.igem.org/mediawiki/2017/5/5b/T--INSA-UPS_France--Logo_fsdie.png" alt="" class="logo-sponsor"></a>
        The fact that literature described the robustness of <i>Pichia pastoris</i><sup>16,17</sup> for the production of antimicrobial peptide made us definitively enthousiast about using this yeast as part of the core.  
+
          <h2><a href="http://www.univ-tlse3.fr/associations-+/do-you-have-a-project--378066.kjsp?RH=1238417866394">FSDIE (Fonds de Soutient de la Division de Vie Etudiante)</a></h2>
      </p>
+
            <p>
      <img src="https://static.igem.org/mediawiki/2017/0/0b/T--INSA-UPS_France--Description-kill-MICAMP.png" alt="" class="right-img">
+
              The <b>Paul Sabatier University</b> encourages and supports initiative and commitment through the The Student Initiatives Solidarity and Development Fund. It helps realise projects and can provide funding for initiatives in various area such as humanitarian action, campus life and scientific culture.
    </section>
+
            </p>          
 
+
          </td>
    <div class="article_offset" id="a6"></div>
+
        </tr>
    <section style="background: none;">
+
        <tr>
      <h1 style="text-align:left;">Our system</h1>    
+
          <td class="section-100" colspan="2">           
      <!--<p>
+
            <a href="http://en.univ-toulouse.fr/our-strengths"><img src="https://static.igem.org/mediawiki/2017/9/93/T--INSA-UPS_France--Logo_fsie.jpg" alt="" class="logo-sponsor"></a>
        See design for more informations of the genetic engineering we used!
+
            <h2><a href="http://en.univ-toulouse.fr/our-strengths">Higher Education and Research in Toulouse Midi-Pyr&eacute;n&eacute;es</a></h2>
      </p>-->
+
            <p>
      <img src="https://static.igem.org/mediawiki/2017/b/b8/T--INSA-UPS_France--description_loop.png" alt="">    
+
              <b>Toulouse University</b> brings together most public institutions of higher education and research in Toulouse and the Midi-Pyr&eacute;n&eacute;es region. Composed of 4 universities and 17 schools of engineering or other specialized subjects, the University&rsquo;s missions are to encourage inter-institutional actions and initiatives in various areas: teaching, student life, science communication, or communication technology.
    </section>
+
            </p>          
 
+
          </td>
    </div>
+
        </tr>
    <!-- fin section -->  
+
        <tr>
 +
          <td class="section-100" colspan="2">
 +
            <a href="https://www.crous-toulouse.fr/english/"><img src="https://static.igem.org/mediawiki/2017/3/37/T--INSA-UPS_France--Logo_crous.png" alt="" class="logo-sponsor"  style="width:150px !important;"></a>
 +
            <p>
 +
              <b>The Centre R&eacute;gional des Oeuvres Universitaires et Scolaires (CROUS)</b> is a public facility controlled by the Ministery of Higher Education and Research. The main goal is to promote the good living conditions of students. The CROUS can provide help in many different actions: direct help through financial fundings, undirect help with housing and catering.
 +
            </p>           
 +
          </td>
 +
        </tr>
 +
    </table>
 +
     
  
 
   </div>
 
   </div>
 
   </div>
 
   </div>
    <style>
+
  <style>
  
 
footer{
 
footer{
Line 389: Line 503:
 
   </main>
 
   </main>
  
<!--
 
  -->
 
  
 
</div>
 
</div>
  
  
 
 
<!-- C O N T E N T -->
 
 
<script type="text/javascript">
 
<script type="text/javascript">
 
   $('.js-sticky').addClass('is-sticky');
 
   $('.js-sticky').addClass('is-sticky');
 
</script>
 
</script>
  
 
<script type="text/javascript">
 
  $('.main_content').scroll(function(){
 
    console.log($('.main_content').scrollTop());
 
    console.log("position 1 :");
 
    console.log($("#a1").position().top);
 
    console.log("position 2 :");
 
    console.log($("#a2").position().top);
 
    var pos = $('.main_content').scrollTop();
 
    // BOLD ASIDE NAV
 
    if(pos>$("#a1").position().top-10){
 
      $('*[data-number="1"]').css("font-weight", "bold");
 
      $('*[data-number="1"]').parent().addClass("selected-item");
 
      $('*[data-number="2"]').css("font-weight", "normal");
 
      $('*[data-number="2"]').parent().removeClass("selected-item");
 
      $('*[data-number="3"]').css("font-weight", "normal");
 
      $('*[data-number="3"]').parent().removeClass("selected-item");
 
      $('*[data-number="4"]').css("font-weight", "normal");
 
      $('*[data-number="4"]').parent().removeClass("selected-item");
 
      $('*[data-number="5"]').css("font-weight", "normal");
 
      $('*[data-number="5"]').parent().removeClass("selected-item");
 
      $('*[data-number="6"]').css("font-weight", "normal");
 
      $('*[data-number="6"]').parent().removeClass("selected-item");
 
      if(pos>$("#a2").position().top-10){
 
        $('*[data-number="1"]').css("font-weight", "normal");
 
        $('*[data-number="1"]').parent().removeClass("selected-item");
 
        $('*[data-number="2"]').css("font-weight", "bold");
 
        $('*[data-number="2"]').parent().addClass("selected-item");
 
        $('*[data-number="3"]').css("font-weight", "normal");
 
        $('*[data-number="3"]').parent().removeClass("selected-item");
 
        $('*[data-number="4"]').css("font-weight", "normal");
 
        $('*[data-number="4"]').parent().removeClass("selected-item");
 
        $('*[data-number="5"]').css("font-weight", "normal");
 
        $('*[data-number="5"]').parent().removeClass("selected-item");
 
        $('*[data-number="6"]').css("font-weight", "normal");
 
        $('*[data-number="6"]').parent().removeClass("selected-item");
 
        if(pos>$("#a3").position().top-10){
 
          $('*[data-number="1"]').css("font-weight", "normal");
 
          $('*[data-number="1"]').parent().removeClass("selected-item");
 
          $('*[data-number="2"]').css("font-weight", "normal");
 
          $('*[data-number="2"]').parent().removeClass("selected-item");
 
          $('*[data-number="3"]').css("font-weight", "bold");
 
          $('*[data-number="3"]').parent().addClass("selected-item");
 
          $('*[data-number="4"]').css("font-weight", "normal");
 
          $('*[data-number="4"]').parent().removeClass("selected-item");
 
          $('*[data-number="5"]').css("font-weight", "normal");
 
          $('*[data-number="5"]').parent().removeClass("selected-item");
 
          $('*[data-number="6"]').css("font-weight", "normal");
 
          $('*[data-number="6"]').parent().removeClass("selected-item");
 
          if(pos>$("#a4").position().top-10){
 
            $('*[data-number="1"]').css("font-weight", "normal");
 
            $('*[data-number="1"]').parent().removeClass("selected-item");
 
            $('*[data-number="2"]').css("font-weight", "normal");
 
            $('*[data-number="2"]').parent().removeClass("selected-item");
 
            $('*[data-number="3"]').css("font-weight", "normal");
 
            $('*[data-number="3"]').parent().removeClass("selected-item");
 
            $('*[data-number="4"]').css("font-weight", "bold");
 
            $('*[data-number="4"]').parent().addClass("selected-item");
 
            $('*[data-number="5"]').css("font-weight", "normal");
 
            $('*[data-number="5"]').parent().removeClass("selected-item");
 
            $('*[data-number="6"]').css("font-weight", "normal");
 
            $('*[data-number="6"]').parent().removeClass("selected-item");
 
            if(pos>$("#a5").position().top-10){
 
              $('*[data-number="1"]').css("font-weight", "normal");
 
              $('*[data-number="1"]').parent().removeClass("selected-item");
 
              $('*[data-number="2"]').css("font-weight", "normal");
 
              $('*[data-number="2"]').parent().removeClass("selected-item");
 
              $('*[data-number="3"]').css("font-weight", "normal");
 
              $('*[data-number="3"]').parent().removeClass("selected-item");
 
              $('*[data-number="4"]').css("font-weight", "normal");
 
              $('*[data-number="4"]').parent().removeClass("selected-item");
 
              $('*[data-number="5"]').css("font-weight", "bold");
 
              $('*[data-number="5"]').parent().addClass("selected-item");
 
              $('*[data-number="6"]').css("font-weight", "normal");
 
              $('*[data-number="6"]').parent().removeClass("selected-item");
 
              if(pos>$("#a6").position().top-10){
 
                $('*[data-number="1"]').css("font-weight", "normal");
 
                $('*[data-number="1"]').parent().removeClass("selected-item");
 
                $('*[data-number="2"]').css("font-weight", "normal");
 
                $('*[data-number="2"]').parent().removeClass("selected-item");
 
                $('*[data-number="3"]').css("font-weight", "normal");
 
                $('*[data-number="3"]').parent().removeClass("selected-item");
 
                $('*[data-number="4"]').css("font-weight", "normal");
 
                $('*[data-number="4"]').parent().removeClass("selected-item");
 
                $('*[data-number="5"]').css("font-weight", "normal");
 
                $('*[data-number="5"]').parent().removeClass("selected-item");
 
                $('*[data-number="6"]').css("font-weight", "bold");
 
                $('*[data-number="6"]').parent().addClass("selected-item");
 
              }
 
            }
 
          }
 
        }
 
      }
 
    }
 
    // FIXED ASIDE NAV
 
    if(pos>371){
 
      $('.left_container').addClass("aside-fixed");
 
    }
 
    else{
 
      $('.left_container').removeClass("aside-fixed");
 
    }
 
  });
 
 
</script>
 
 
<script type="text/javascript">
 
 
    // NAV BAR SUBNAV CLICK
 
 
  $(document).on("click",".cat_name", function(){
 
    if($(this).parent().children('.subnav').css("max-height")=="0px"){
 
      $('.subnav').css("max-height","0px");
 
      $(this).parent().children('.subnav').css("max-height","1000px");
 
    }
 
    else{
 
      $(this).parent().children('.subnav').css("max-height","0px");
 
    }   
 
  });
 
</script>
 
 
<script type="text/javascript">
 
  var contentSections = $('.article_offset'),
 
  navigationItems = $('.left_container .aside-nav__item:after');
 
 
  updateNavigation();
 
  $('.main_content').on('scroll', function(){
 
    updateNavigation();
 
    });
 
 
 
  function updateNavigation() {
 
    contentSections.each(function(){
 
      $this = $(this);
 
 
      var activeSection = $('.left_container a[href="#'+$this.attr('id')+'"]').data('number') - 1;
 
    });
 
  }
 
 
  function smoothScroll(target) {
 
        $('body,html').animate(
 
          {'scrollTop':target.offset().top},
 
          600
 
        );
 
  }
 
</script>
 
 
</body>
 
 
</html>
 
</html>
 
{{INSA-UPS_France/General_script}}
 
{{INSA-UPS_France/General_script}}
 
{{INSA-UPS_France/Header_script}}
 
{{INSA-UPS_France/Header_script}}

Revision as of 08:06, 24 September 2017

Supports