Difference between revisions of "Team:Cardiff Wales/basicparts"

Line 24: Line 24:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>A protein coding sequence of a gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does not</b> have His-tags for purification </td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 31: Line 31:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td> A protein coding sequence of a gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does</b> have His-tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 38: Line 38:
 
     <td>PDF1.2</td>
 
     <td>PDF1.2</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>An inducible promoter that responds to the presence of jasmonic acid in the cell</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 45: Line 45:
 
     <td>PR2</td>
 
     <td>PR2</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 52: Line 52:
 
     <td>GST6</td>
 
     <td>GST6</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 59: Line 59:
 
     <td>WRKY30</td>
 
     <td>WRKY30</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>An inducible promoter that responds to the presence of cellulose-derived oligomers in the cell</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 66: Line 66:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate,
 +
luciferin, which can be used to quantify gene expression </td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 73: Line 74:
 
     <td>PR2-PDF1.2</td>
 
     <td>PR2-PDF1.2</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td> A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the jasmonic acid responsive PDF1.2 at the most 3' end</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 80: Line 81:
 
     <td>PR2-GST6</td>
 
     <td>PR2-GST6</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 87: Line 88:
 
     <td>PDF1.2-GST6</td>
 
     <td>PDF1.2-GST6</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>A composite promoter of both the jasmonic acid responsive PDF1.2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 94: Line 95:
 
     <td>NosT</td>
 
     <td>NosT</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>A terminator from the nopaline synthase gene which is native to the  <i> Agrobacterium </i> tumor-inducing plasmid pTiT37</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>
Line 101: Line 102:
 
     <td>LexA</td>
 
     <td>LexA</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>An inducible promoter from the LexA gene which is native to <i>Escherichia coli </i> </td>
−
<td>~length</td>
+
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 108: Line 108:
 
     <td>35S</td>
 
     <td>35S</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td></td>
+
     <td>A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus</td>
 
<td>~length</td>
 
<td>~length</td>
 
   </tr>
 
   </tr>

Revision as of 13:07, 26 September 2017




Basic Parts






Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.


Registry link Part Plasmid Summary Approximate Length
BBa_K2404000 TSH pSB1C3 A protein coding sequence of a gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification ~length
BBa_K2404001 TSHH pSB1C3 A protein coding sequence of a gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification ~length
BBa_K2404002 PDF1.2 pSB1C3 An inducible promoter that responds to the presence of jasmonic acid in the cell ~length
BBa_K2404003 PR2 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell ~length
BBa_K2404004 GST6 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell ~length
BBa_K2404005 WRKY30 pSB1C3 An inducible promoter that responds to the presence of cellulose-derived oligomers in the cell ~length
BBa_K2404006 Luc+ pSB1C3 A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression ~length
BBa_K2404007 PR2-PDF1.2 pSB1C3 A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the jasmonic acid responsive PDF1.2 at the most 3' end ~length
BBa_K2404008 PR2-GST6 pSB1C3 A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end ~length
BBa_K2404009 PDF1.2-GST6 pSB1C3 A composite promoter of both the jasmonic acid responsive PDF1.2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end ~length
BBa_K2404010 NosT pSB1C3 A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 ~length
BBa_K2404011 LexA pSB1C3 An inducible promoter from the LexA gene which is native to Escherichia coli
BBa_K2404012 35S pSB1C3 A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus ~length