Difference between revisions of "Team:Aix-Marseille/KXconcept"

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{{Aix-Marseille|title=KILL XYL|toc=__TOC__}}
 
  
At Aix-Marseille University we thought about a solution that enclose many aspect of the cure.
 
 
First, we wanted to improve the detection of the disease, to do so we wanted to use a NDVI camera that will help us to see if the plant do photosynthesis or not. If the plant is heavily affected by ''Xylella fastidiosa'', it support a hydric stress that stop the photosynthesis.
 
 
Secondly, we want to get rid of the bacterium. Phages are natural predators of bacteria. They can also be used to transfect DNA into a bacterial cell. Phages has also the advantage of being specific to a strain and to be modulable. As we wanted to be eco-friendly, we create phage-likes particles, that aren't able to spread. Thus we have nanobots specific to ''Xylella fastidiosa'', capable to inject toxic genes into the bacterium.
 
 
The main cause of the plants death, is the hydric stress induced by the accumulation of biofilm into the xylem vessels. To disrupt the biofilm we thought about different solutions. The first one is to stop the bacterium producing any extra poly-saccharide. This could be achieved by quenching the quorum sensing of the bacterium with the help of a little fatty acid called : 2-cis-decenoic acid. Secondly, we wanted to destroy the exo-polysaccharids. An enzyme coming from a bacteriophage could fulfill the use by the hydrolysis of polysaccharides.
 
 
Hence, '''KILL XYLL''' simply detects, disrupt and kill ''Xylella fastidiosa''.
 
 
===Module 1:  Detection of the disease===
 
 
===Module 2 : Engineering bacteriophages===
 
 
[[File:T--Aix-Marseille--Phage.png|400px|right|thumb|Phage-like particles process.]]
 
 
Bacteriophages play a special role in nanoscale cargo-delivery developments, because they can be regarded as naturally occurring nanomaterials. Viral nanoparticles (VNPs), in particular bacteriophages, are attractive options for cargo-delivery as they are biocompatible, biodegradable, and non-infectious to mammals.
 
 
Phage systems, like M13, have been employed in biotechnological applications, most prominently in the identification and maturation of medically-relevant binding molecules through phage display. The application of phages in materials and nanotechnology is mainly due to their nanoscale size and simple life cycles. We choose to use those application in our advantage in order to target ''Xylella fastidiosa'' and other pathogenic bacteria.
 
 
[[Team:Aix-Marseille/Bacteriophages|Read more…]]
 
 
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===Module 3 : Quorum sensing approach===
 
 
''Xylella fastidiosa'' uses quorum sensing, as an inter-bacterial communication system, to regulate its biofilm production. The quorum sensing is based on the emission of specific fatty acid, there are fatty acids antagonistic to its quorum sensing, this disturbance is called quorum quenching. 2-cis-decenoic acid is one of these fatty acids whom has quorum quenching effect to ''Xylella fastidiosa''. This action of quenching will prevent the formation of biofilm, dependent of the quorum sensing and therefore will have both action preventive and curative to the symptoms caused by the biofilm on the plants. We want to produce this fatty acids from ''E. coli'' to inoculated the infected trees. Thus saving the plants from ''Xylella fastidiosa''.
 
 
[[Team:Aix-Marseille/QS|Read more…]]
 
 
===Module 4 : Disrupting the biofilm===
 
 
Extracellular polysaccharide (EPS) is a major virulence factor of Xylella Fastidiosa, the causative agent of Olive Quick Decline (OQD) associated with binding of water, ions and nutrients; keeping them in close contact with the bacteria; and protection against the recognition by plant cell defense mechanisms.
 
Some bacteriophages carry coat proteins that can degrade bacterial polysaccharides. The Depolymerase binds to the capsular EPS and degrades the polymer until the phage reaches the cell surface, where it binds to an outer membrane receptor and injects nucleic acid to initiate the lytic cycle.
 
To disrupt the biofilm we centered our research on this enzyme, which we called deps to cleave the extracellular polysaccharides of Xylella Fastidiosa freeing up the tension cause by EPS in the xylem vessels. I hope that curing the plant from the hydric stress caused by the bacteria.
 
 
 
[[Team:Aix-Marseille/DEPS|Read more…]]
 

Latest revision as of 10:18, 28 September 2017