Difference between revisions of "Team:IISER-Mohali-INDIA/InterLab"

Line 36: Line 36:
 
}
 
}
 
],
 
],
 +
"guides": [],
 +
"valueAxes": [
 +
{
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"axisTitleOffset": 15,
 +
"id": "ValueAxis-1",
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"autoRotateCount": 0,
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"axisAlpha": 0,
 +
"minHorizontalGap": 73,
 +
"offset": -2
 +
},
 +
{
 +
"axisTitleOffset": 15,
 +
"id": "ValueAxis-2",
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"position": "bottom",
 +
"axisAlpha": 0
 +
},
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{
 +
"id": "ValueAxis-3"
 +
}
 +
],
 +
"allLabels": [
 +
{
 +
"id": "Label-1",
 +
"rotation": -90,
 +
"text": "Fluorescence",
 +
"x": "1%",
 +
"y": "55%"
 +
},
 +
{
 +
"align": "center",
 +
"id": "Label-2",
 +
"tabIndex": 1,
 +
"text": "Fluorescein Concentration(uM)",
 +
"x": "-7%",
 +
"y": "93%"
 +
}
 +
],
 +
"balloon": {},
 +
"titles": [
 +
{
 +
"bold": false,
 +
"id": "Title-1",
 +
"text": "Fluorescein Standard Curve "
 +
}
 +
],
 +
"dataProvider": [
 +
{
 +
"Fluorescence": "63471.5",
 +
"Conc.(uM)": "50"
 +
},
 +
{
 +
"Fluorescence": "45389.75",
 +
"Conc.(uM)": "25"
 +
},
 +
{
 +
"Fluorescence": "30206.75",
 +
"Conc.(uM)": "12.5"
 +
},
 +
{
 +
"Fluorescence": "18120.75",
 +
"Conc.(uM)": "6.25"
 +
},
 +
{
 +
"Fluorescence": "9627.75",
 +
"Conc.(uM)": "3.125"
 +
},
 +
{
 +
"Fluorescence": "4863",
 +
"Conc.(uM)": "1.5625"
 +
},
 +
{
 +
"Fluorescence": "2540.5",
 +
"Conc.(uM)": "0.78125"
 +
},
 +
{
 +
"Fluorescence": "1354",
 +
"Conc.(uM)": "0.390625"
 +
},
 +
{
 +
"Fluorescence": "692",
 +
"Conc.(uM)": "0.195313"
 +
},
 +
{
 +
"Fluorescence": "342.75",
 +
"Conc.(uM)": "0.097656"
 +
},
 +
{
 +
"Fluorescence": "175.25",
 +
"Conc.(uM)": "0.048828"
 +
},
 +
{
 +
"Fluorescence": "5.75",
 +
"Conc.(uM)": "0"
 +
}
 +
]
 +
}
 +
);
 +
AmCharts.makeChart1("chartdiv1",
 +
{
 +
"type": "xy",
 +
"minValue": -4,
 +
"autoMarginOffset": 20,
 +
"startDuration": 1.5,
 +
"processTimeout": -2,
 +
"theme": "chalk",
 +
"trendLines": [],
 +
"graphs": [
 +
{
 +
"balloonText": "x:<b>[[x]]</b> y:<b>[[y]]</b><br>value:<b>[[value]]</b>",
 +
"bullet": "diamond",
 +
"id": "AmGraph-1",
 +
"lineColor": "#b0de09",
 +
"valueField": "value",
 +
"xField": "Conc.(uM)",
 +
"yField": "Fluorescence"
 +
}
 +
],
 +
                                          yaxis:[ {
 +
    type: 'log',
 +
    autorange: true
 +
  }
 +
],
 
"guides": [],
 
"guides": [],
 
"valueAxes": [
 
"valueAxes": [
Line 441: Line 563:
  
 
</div>
 
</div>
−
<div id="chartdiv" style="width: 50%; height: 400px; background-color: #282828;" ></div>
+
<div id="chartdiv" style="width: 50%; height: 400px; background-color: #282828;" >
 +
<div id="chartdiv1" style="width: 50%; height: 400px; background-color: #282828;" ></div>
 +
</div>
 
</html>
 
</html>

Revision as of 21:28, 28 September 2017

<!DOCTYPE html>



OD600 Reference Point

  LUDOX-HS40 H2O
Replicate 1 0.0932 0.0973
Replicate 2 0.0927 0.0777
Replicate 3 0.0961 0.0796
Replicate 4 0.0937 0.0803
Arith. Mean 0.093925 0.083725
Corrected Abs600 0.0102
Reference OD600 0.0425
OD600/Abs600 4.166666667

Fluorescence Standard Curves


uM Fluorescein

uM Fluorescein 50.00 25 12.5 6.25 3.125 1.5625 0.78125 0.390625 0.1953125 0.09765625 0.048828125 0
Replicate 1 60105 43268 29004 17671 9656 4867 2466 1328 658 324 160 6
Replicate 2 64328 45105 30375 18411 9892 4742 2569 1361 698 339 172 6
Replicate 3 64879 46230 30542 18336 9472 4772 2540 1353 703 349 179 5
Replicate 4 64574 46956 30906 18065 9491 5071 2587 1374 709 359 190 6
Arith. Mean 63471.5 45389.75 30206.75 18120.75 9627.75 4863 2540.5 1354 692 342.75 175.25 5.75
Arith. Std.Dev. 2255.620905 1606.452069 831.9172134 334.6444631 194.5805317 148.550777 53.30728531 19.37352145 23.10844002 14.93039406 12.57974563 0.5

uM Fluorescein/a.u.


uM Fluorescein/a.u. 50.00 25 12.5 6.25 3.125 1.5625 0.78125 0.390625 0.1953125 0.09765625 0.048828125
Mean um Fluorescein/a.u. 0.000787755 0.000550785 0.000413815 0.000344908 0.000324583 0.000321304 0.000307518 0.000288497 0.000282243 0.00028492 0.00027862
Mean of med-high levels: 0.000391079