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Revision as of 22:18, 28 September 2017

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OD600 Reference Point

  LUDOX-HS40 H2O
Replicate 1 0.0932 0.0973
Replicate 2 0.0927 0.0777
Replicate 3 0.0961 0.0796
Replicate 4 0.0937 0.0803
Arith. Mean 0.093925 0.083725
Corrected Abs600 0.0102
Reference OD600 0.0425
OD600/Abs600 4.166666667

Fluorescence Standard Curves


uM Fluorescein

uM Fluorescein 50.00 25 12.5 6.25 3.125 1.5625 0.78125 0.390625 0.1953125 0.09765625 0.048828125 0
Replicate 1 60105 43268 29004 17671 9656 4867 2466 1328 658 324 160 6
Replicate 2 64328 45105 30375 18411 9892 4742 2569 1361 698 339 172 6
Replicate 3 64879 46230 30542 18336 9472 4772 2540 1353 703 349 179 5
Replicate 4 64574 46956 30906 18065 9491 5071 2587 1374 709 359 190 6
Arith. Mean 63471.5 45389.75 30206.75 18120.75 9627.75 4863 2540.5 1354 692 342.75 175.25 5.75
Arith. Std.Dev. 2255.620905 1606.452069 831.9172134 334.6444631 194.5805317 148.550777 53.30728531 19.37352145 23.10844002 14.93039406 12.57974563 0.5

uM Fluorescein/a.u.


uM Fluorescein/a.u. 50.00 25 12.5 6.25 3.125 1.5625 0.78125 0.390625 0.1953125 0.09765625 0.048828125
Mean um Fluorescein/a.u. 0.000787755 0.000550785 0.000413815 0.000344908 0.000324583 0.000321304 0.000307518 0.000288497 0.000282243 0.00028492 0.00027862
Mean of med-high levels: 0.000391079