Difference between revisions of "Team:Cardiff Wales/compositeparts"

Line 14: Line 14:
 
     <th>Coding sequence</th>  
 
     <th>Coding sequence</th>  
 
     <th>Terminator</th>
 
     <th>Terminator</th>
−
     <th>Plasmid</th>
+
     <th>Plasmid backbone</th>
 
     <th>Summary</th>
 
     <th>Summary</th>
 
     <th>Approximate Length</th>
 
     <th>Approximate Length</th>
Line 23: Line 23:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A constitutive promoter with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A constitutive promoter with our TSH antagonist <b>without</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 31: Line 31:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A constitutive promoter with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A constitutive promoter with our TSH antagonist <b>with</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 39: Line 39:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A constitutive promoter with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A constitutive promoter with Luc+ as a coding sequence to analyse expression levels</td>
 
<td>~length</td>
 
<td>~length</td>
Line 47: Line 47:
 
     <td>Lucx5</td>
 
     <td>Lucx5</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
<td>A constitutive promoter with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant</td>
 
<td>A constitutive promoter with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant</td>
 
<td>~length</td>
 
<td>~length</td>
Line 55: Line 55:
 
     <td>XVE</td>
 
     <td>XVE</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
<td>A constitutive promoter with XVE as a coding sequence. This produces a protein product that activates the inducible promoter LexA</td>
 
<td>A constitutive promoter with XVE as a coding sequence. This produces a protein product that activates the inducible promoter LexA</td>
 
<td>~length</td>
 
<td>~length</td>
Line 63: Line 63:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter that is induced by XVE with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A promoter that is induced by XVE with our TSH antagonist <b>without</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 71: Line 71:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter that is induced by XVE  with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A promoter that is induced by XVE  with our TSH antagonist <b>with</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 79: Line 79:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter that is induced by XVE  with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A promoter that is induced by XVE  with Luc+ as a coding sequence to analyse expression levels</td>
 
<td>~length</td>
 
<td>~length</td>
Line 87: Line 87:
 
     <td>Lucx5</td>
 
     <td>Lucx5</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
<td>A promoter that is induced by XVE  with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant</td>
 
<td>A promoter that is induced by XVE  with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant</td>
 
<td>~length</td>
 
<td>~length</td>
Line 95: Line 95:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by jasmonic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A promoter induced by jasmonic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 104: Line 104:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by jasmonic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A promoter induced by jasmonic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 112: Line 112:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by jasmonic acid with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A promoter induced by jasmonic acid with Luc+ as a coding sequence to analyse expression levels</td>
 
<td>~length</td>
 
<td>~length</td>
Line 120: Line 120:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 128: Line 128:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 136: Line 136:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels</td>
 
<td>~length</td>
 
<td>~length</td>
Line 144: Line 144:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>without</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 152: Line 152:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A promoter induced by salicylic acid with our TSH antagonist <b>with</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 160: Line 160:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels</td>
 
<td>~length</td>
 
<td>~length</td>
Line 168: Line 168:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist <b>without</b> the his tags for purification</td>
 
     <td>A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist <b>without</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 176: Line 176:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist <b>with</b> the his tags for purification</td>
 
     <td>A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist <b>with</b> the his tags for purification</td>
 
<td>~length</td>
 
<td>~length</td>
Line 184: Line 184:
 
     <td>Luc+</td>
 
     <td>Luc+</td>
 
     <td>NosT</td>
 
     <td>NosT</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 
     <td>A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels</td>
 
     <td>A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels</td>
 
<td>~length</td>
 
<td>~length</td>

Revision as of 18:51, 29 September 2017




Composite Parts






Promotor Coding sequence Terminator Plasmid backbone Summary Approximate Length
35S TSH NosT pGB-A2 A constitutive promoter with our TSH antagonist without the his tags for purification ~length
35S TSHH NosT pGB-A2 A constitutive promoter with our TSH antagonist with the his tags for purification ~length
35S Luc+ NosT pGB-A2 A constitutive promoter with Luc+ as a coding sequence to analyse expression levels ~length
35S Lucx5 NosT pGB-A2 A constitutive promoter with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant ~length
35S XVE NosT pGB-A2 A constitutive promoter with XVE as a coding sequence. This produces a protein product that activates the inducible promoter LexA ~length
LexA TSH NosT pGB-A2 A promoter that is induced by XVE with our TSH antagonist without the his tags for purification ~length
LexA TSHH NosT pGB-A2 A promoter that is induced by XVE with our TSH antagonist with the his tags for purification ~length
LexA Luc+ NosT pGB-A2 A promoter that is induced by XVE with Luc+ as a coding sequence to analyse expression levels ~length
LexA Lucx5 NosT pGB-A2 A promoter that is induced by XVE with Lucx5 as a coding sequence to analyse expression levels. This variant is heat resistant ~length
PDF1.2 TSH NosT pGB-A2 A promoter induced by jasmonic acid with our TSH antagonist without the his tags for purification ~length
PDF1.2 TSHH NosT pGB-A2 A promoter induced by jasmonic acid with our TSH antagonist with the his tags for purification ~length
PDF1.2 Luc+ NosT pGB-A2 A promoter induced by jasmonic acid with Luc+ as a coding sequence to analyse expression levels ~length
PR2 TSH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist without the his tags for purification ~length
PR2 TSHH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist with the his tags for purification ~length
PR2 Luc+ NosT pGB-A2 A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels ~length
GST6 TSH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist without the his tags for purification ~length
GST6 TSHH NosT pGB-A2 A promoter induced by salicylic acid with our TSH antagonist with the his tags for purification ~length
GST6 Luc+ NosT pGB-A2 A promoter induced by salicylic acid with Luc+ as a coding sequence to analyse expression levels ~length
WRKY30 TSH NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist without the his tags for purification ~length
WRKY30 TSHH NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with our TSH antagonist with the his tags for purification ~length
WRKY30 Luc+ NosT pGB-A2 A promoter induced by the presence of cellulose (DAMP response) with Luc+ as a coding sequence to analyse expression levels ~length