Difference between revisions of "Team:Florida Atlantic/InterLab"

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<h3>★  ALERT! </h3>
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<center><h1>Florida Atlantic Interlap Report</h1></center>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
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<h4>Member Participations</h4>
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<h5>Calibrations:</h5>
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- Completed by Douglas + Valentina on September 21st
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<h5>Transformations:</h5>
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- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th
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<h5>Cell Measurement:</h5>
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- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 29th
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<h4>Methodology</h4>
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<h5>Materials</h5>
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Competent cells (Escherichia coli strain DH5α)
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LB (Luria Bertani) media
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<br/>
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Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)
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<br/>
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50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)
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<br/>
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Incubator at 37°C
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<br/>
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1.5 ml eppendorf tubes for sample storage
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<br/>
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Ice bucket with ice
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Pipettes
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96 well plate, black with flat, transparent/clear bottom preferred (provided by team)
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<h6>Devices (from InterLab Measurement Kit): </h6>
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● Positive control
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● Negative control
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● Test Device 1: J23101+I13504
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<br/>
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● Test Device 2: J23106+I13504
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● Test Device 3: J23117+I13504
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● Test Device 4: J23101.BCD2.E0040.B0015
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● Test Device 5: J23106.BCD2.E0040.B0015
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● Test Device 6: J23117.BCD2.E0040.B0015
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<h5>Calibration Procedure</h5>
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<h5>Transformation Procedure</h5>
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<h5>Cell Measurement Procedure</h5>
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Revision as of 20:36, 29 September 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
InterLab
Model
Results
Attributions
Pictures
HUMAN PRACTICES
Silver HP
Gold HP
AWARDS
Model
Software
SAFETY
JUDGING FORM

Florida_Atlantic

Florida Atlantic Interlap Report


Member Participations


Calibrations:
- Completed by Douglas + Valentina on September 21st
Transformations:
- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th
Cell Measurement:
- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 29th

Methodology


Materials

Competent cells (Escherichia coli strain DH5α)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)
Incubator at 37°C
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate, black with flat, transparent/clear bottom preferred (provided by team)
Devices (from InterLab Measurement Kit):
● Positive control
● Negative control
● Test Device 1: J23101+I13504
● Test Device 2: J23106+I13504
● Test Device 3: J23117+I13504
● Test Device 4: J23101.BCD2.E0040.B0015
● Test Device 5: J23106.BCD2.E0040.B0015
● Test Device 6: J23117.BCD2.E0040.B0015

Calibration Procedure

Transformation Procedure

Cell Measurement Procedure