(Prototype team page) |
Rstclair2012 (Talk | contribs) |
||
Line 1: | Line 1: | ||
{{Florida_Atlantic}} | {{Florida_Atlantic}} | ||
+ | |||
<html> | <html> | ||
− | < | + | <form><fieldset> |
− | < | + | <center><h1>Florida Atlantic Interlap Report</h1></center> |
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | <h1> | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </ | + | |
+ | <br/> | ||
+ | <h4>Member Participations</h4> | ||
+ | <br/> | ||
+ | <h5>Calibrations:</h5> | ||
+ | - Completed by Douglas + Valentina on September 21st | ||
+ | <br/> | ||
+ | <h5>Transformations:</h5> | ||
+ | - Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th | ||
+ | <br/> | ||
+ | <h5>Cell Measurement:</h5> | ||
+ | - Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 29th | ||
+ | <br/> | ||
+ | <br/> | ||
+ | <h4>Methodology</h4> | ||
+ | <br/> | ||
+ | <h5>Materials</h5> | ||
+ | <br/> | ||
+ | Competent cells (Escherichia coli strain DH5α) | ||
+ | <br/> | ||
+ | LB (Luria Bertani) media | ||
+ | <br/> | ||
+ | Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL) | ||
+ | <br/> | ||
+ | 50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light) | ||
+ | <br/> | ||
+ | Incubator at 37°C | ||
+ | <br/> | ||
+ | 1.5 ml eppendorf tubes for sample storage | ||
+ | <br/> | ||
+ | Ice bucket with ice | ||
+ | <br/> | ||
+ | Pipettes | ||
+ | <br/> | ||
+ | 96 well plate, black with flat, transparent/clear bottom preferred (provided by team) | ||
+ | <br/> | ||
+ | <h6>Devices (from InterLab Measurement Kit): </h6> | ||
+ | ● Positive control | ||
+ | <br/> | ||
+ | ● Negative control | ||
+ | <br/> | ||
+ | ● Test Device 1: J23101+I13504 | ||
+ | <br/> | ||
+ | ● Test Device 2: J23106+I13504 | ||
+ | <br/> | ||
+ | ● Test Device 3: J23117+I13504 | ||
+ | <br/> | ||
+ | ● Test Device 4: J23101.BCD2.E0040.B0015 | ||
+ | <br/> | ||
+ | ● Test Device 5: J23106.BCD2.E0040.B0015 | ||
+ | <br/> | ||
+ | ● Test Device 6: J23117.BCD2.E0040.B0015 | ||
+ | <br/> | ||
+ | <br/> | ||
+ | <h5>Calibration Procedure</h5> | ||
+ | <br/> | ||
+ | <h5>Transformation Procedure</h5> | ||
+ | <br/> | ||
+ | <h5>Cell Measurement Procedure</h5> | ||
+ | <br/> | ||
+ | </div> | ||
</html> | </html> |
Revision as of 20:36, 29 September 2017
Florida_Atlantic