Difference between revisions of "Team:Florida Atlantic/InterLab"

Line 23: Line 23:
 
<br/>
 
<br/>
 
<h5>Materials</h5>
 
<h5>Materials</h5>
−
<br/>
 
 
Competent cells (Escherichia coli strain DH5α)  
 
Competent cells (Escherichia coli strain DH5α)  
 
<br/>
 
<br/>

Revision as of 20:37, 29 September 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
InterLab
Model
Results
Attributions
Pictures
HUMAN PRACTICES
Silver HP
Gold HP
AWARDS
Model
Software
SAFETY
JUDGING FORM

Florida_Atlantic

Florida Atlantic Interlap Report


Member Participations


Calibrations:
- Completed by Douglas + Valentina on September 21st
Transformations:
- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th
Cell Measurement:
- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 29th

Methodology


Materials
Competent cells (Escherichia coli strain DH5α)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)
Incubator at 37°C
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate, black with flat, transparent/clear bottom preferred (provided by team)
Devices (from InterLab Measurement Kit):
● Positive control
● Negative control
● Test Device 1: J23101+I13504
● Test Device 2: J23106+I13504
● Test Device 3: J23117+I13504
● Test Device 4: J23101.BCD2.E0040.B0015
● Test Device 5: J23106.BCD2.E0040.B0015
● Test Device 6: J23117.BCD2.E0040.B0015

Calibration Procedure

Transformation Procedure

Cell Measurement Procedure