Difference between revisions of "Team:Florida Atlantic/InterLab"

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<h1>Florida Atlantic Interlap Report</h1>
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<h1>Interlap Report</h1>
  
 
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<center><h4>Member Participations</h4></center>
 
<center><h4>Member Participations</h4></center>
<h5><center>Calibrations:</h5>
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<h5>Calibrations:</h5>
 
- Completed by Douglas + Valentina on September 21st
 
- Completed by Douglas + Valentina on September 21st
 
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Revision as of 20:41, 29 September 2017

Florida_Atlantic

Interlap Report


Member Participations

Calibrations:
- Completed by Douglas + Valentina on September 21st
Transformations:
- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th
Cell Measurement:
- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 29th

Methodology

Materials
Competent cells (Escherichia coli strain DH5α)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)
Incubator at 37°C
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate, black with flat, transparent/clear bottom preferred (provided by team)
Devices (from InterLab Measurement Kit):
● Positive control
● Negative control
● Test Device 1: J23101+I13504
● Test Device 2: J23106+I13504
● Test Device 3: J23117+I13504
● Test Device 4: J23101.BCD2.E0040.B0015
● Test Device 5: J23106.BCD2.E0040.B0015
● Test Device 6: J23117.BCD2.E0040.B0015

Calibration Procedure

Transformation Procedure

Cell Measurement Procedure