Rstclair2012 (Talk | contribs) |
Rstclair2012 (Talk | contribs) |
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<h5>Transformation Procedure</h5> | <h5>Transformation Procedure</h5> | ||
+ | add 5uL DNA | ||
+ | <br/> | ||
+ | let sit 30 min on ice | ||
+ | <br/> | ||
+ | heat shock at 42C for 30 sec | ||
+ | <br/> | ||
+ | place back on ice for 2 min | ||
+ | <br/> | ||
+ | add 450uL Luria Broth and incubate at 37c for 2 hours | ||
+ | <br/> | ||
+ | plate 100uL on LB/chloramphenicol plates | ||
+ | <br/> | ||
+ | grow overnight at 37C | ||
+ | <br/> | ||
+ | this was done for all 8 machines | ||
<br/> | <br/> | ||
Revision as of 21:53, 29 September 2017
Florida_Atlantic