Difference between revisions of "Team:Florida Atlantic/InterLab"

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<h5>Transformation Procedure</h5>
 
<h5>Transformation Procedure</h5>
 +
add 5uL DNA
 +
<br/>
 +
let sit 30 min on ice
 +
<br/>
 +
heat shock at 42C for 30 sec
 +
<br/>
 +
place back on ice for 2 min
 +
<br/>
 +
add 450uL Luria Broth and incubate at 37c for 2 hours
 +
<br/>
 +
plate 100uL on LB/chloramphenicol plates
 +
<br/>
 +
grow overnight at 37C
 +
<br/>
 +
this was done for all 8 machines
 
<br/>
 
<br/>
  

Revision as of 21:53, 29 September 2017

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Florida_Atlantic

Interlab Report


Member Participations

Calibrations:
- Completed by Douglas + Valentina on September 21st
Transformations:
- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th
Cell Measurement:
- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 29th

Methodology

Materials
Competent cells (Escherichia coli strain DH5α)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)
Incubator at 37°C
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate, black with flat, transparent/clear bottom preferred (provided by team)
Devices (from InterLab Measurement Kit):
● Positive control
● Negative control
● Test Device 1: J23101+I13504
● Test Device 2: J23106+I13504
● Test Device 3: J23117+I13504
● Test Device 4: J23101.BCD2.E0040.B0015
● Test Device 5: J23106.BCD2.E0040.B0015
● Test Device 6: J23117.BCD2.E0040.B0015

Calibration Procedure

Transformation Procedure
add 5uL DNA
let sit 30 min on ice
heat shock at 42C for 30 sec
place back on ice for 2 min
add 450uL Luria Broth and incubate at 37c for 2 hours
plate 100uL on LB/chloramphenicol plates
grow overnight at 37C
this was done for all 8 machines
Cell Measurement Procedure