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<font size="3">The strength of fluorescence is correlated to the strength of promoter. The higher the strength of promoters, the brighter the colonies. Among the colonies from devices 4-6, the colonies from device 4 show the highest fluorescence while those from device 6 show the lowest. | <font size="3">The strength of fluorescence is correlated to the strength of promoter. The higher the strength of promoters, the brighter the colonies. Among the colonies from devices 4-6, the colonies from device 4 show the highest fluorescence while those from device 6 show the lowest. | ||
Among the colonies from devices 1-3, the colonies from device 3 show the lowest fluorescence but no observable difference in fluorescence between the colonies from devices 1 and 2.</font> </p> | Among the colonies from devices 1-3, the colonies from device 3 show the lowest fluorescence but no observable difference in fluorescence between the colonies from devices 1 and 2.</font> </p> | ||
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<p><img src="https://static.igem.org/mediawiki/2017/e/e5/Screen_Shot_2017-10-03_at_2.05.53_AM.png" style="width:700px;height:400px;" ></p> | <p><img src="https://static.igem.org/mediawiki/2017/e/e5/Screen_Shot_2017-10-03_at_2.05.53_AM.png" style="width:700px;height:400px;" ></p> | ||
+ | <p> | ||
+ | <font size="3"> | ||
+ | We process the data by averaging the values of two colonies picked from each device. For example, the OD600 value of the device 1 in the first graph is the average of the two colonies from device 1. | ||
+ | </font> | ||
+ | </p> | ||
+ | <p> | ||
+ | <font size="3"> | ||
+ | The increasing trends of OD600 and fluorescence in all devices are shown in the first and the second graph. | ||
+ | In the third graph, device 4 has the highest fluorescence per cell and device 6 has the lowest among devices 4-6. Device 4 with the strongest promoter(J23101), thus it has a highest GFP production and give the highest fluorescence. Device 6 have the same ribosome binding site but a weakest promoter(J23117) , lowest fluorescence due to lowest GFP production is observed. Among devices 1-3, device 1 has the highest fluorescence per cell and device 3 has the lowest. Device 1 with the strongest promoter(J23101), thus it has a highest GFP production and give the highest fluorescence. Device 3 have the same ribosome binding site but a weakest promoter(J23117), lowest fluorescence due to lowest GFP production is observed. | ||
+ | </font> </p> | ||
<p> | <p> | ||
− | <font size="3"> | + | <font size="3"> |
+ | For these three pairs [device 1 and 4, device 2 and 5, device 3 and 6], the fluorescence per cell of devices containing bicistronic device (BCD2) [Devices 4,5 and 6] is lower than that of the devices containing B0034 ribosome binding site [Devices 1,2 and 3]. | ||
+ | </font> | ||
+ | </p> | ||
<p> | <p> | ||
<font size="3">Interestingly, the fluorescence per cell of device 6 and negative control are very close. The fluorescence per cell of device 6 is even lower than the negative control at some time points. | <font size="3">Interestingly, the fluorescence per cell of device 6 and negative control are very close. The fluorescence per cell of device 6 is even lower than the negative control at some time points. | ||
This shows that device 6 produces very little amount of GFP and the result may also be affected by the measurement error in plate reader.</font> </p> | This shows that device 6 produces very little amount of GFP and the result may also be affected by the measurement error in plate reader.</font> </p> | ||
<p> | <p> | ||
− | <font size="3">Another interesting point is the fluorescence per cell of all devices peaked at (t= | + | <font size="3">Another interesting point is the fluorescence per cell of all devices peaked at (t=2 hours). One of the possible reasons is the cells spend more energy on cell division but not on producing GFP. Therefore, the cell number increased faster than the GFP production, causing a lower value of fluorescein per cell after (t>2 hours). </font> |
</p> | </p> | ||
Revision as of 10:05, 3 October 2017