Difference between revisions of "Team:INSA-UPS France/Notebook"

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       border:none;
 
       border:none;
 
     }
 
     }
 +
 
     </style>
 
     </style>
 
     <div class="calendar">
 
     <div class="calendar">
Line 148: Line 149:
 
       <h3>January</h3>
 
       <h3>January</h3>
 
       <table>
 
       <table>
−
         <tr data-target="1" class="current-week">
+
         <tr data-target="1">
 
           <td>1</td>
 
           <td>1</td>
 
           <td>2</td>
 
           <td>2</td>
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           <td>21</td>
 
           <td>21</td>
 
         </tr>
 
         </tr>
−
         <tr data-target="4">
+
         <tr data-target="4" class="current-week">
 
           <td>22</td>
 
           <td>22</td>
 
           <td>23</td>
 
           <td>23</td>
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         <style>
 
         <style>
 
     section img{
 
     section img{
−
       width:100%;
+
       width:80%;
 
     }
 
     }
 
     section h1{
 
     section h1{
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     <style>
 
     <style>
 +
 
       .wet-lab-notebook{
 
       .wet-lab-notebook{
 
         display:table;
 
         display:table;
 
       }
 
       }
 +
      .wet-lab-notebook > div{
 +
        display: table-row;
 +
        text-align: center;
 +
      }
 +
 
       .wet-lab-notebook > div > div{
 
       .wet-lab-notebook > div > div{
 
         display:table-cell;
 
         display:table-cell;
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         padding:10px;
 
         padding:10px;
 
       }
 
       }
−
 
+
      .wet-lab-notebook > div > div > img{
 +
        max-width:380px;
 +
      }
 +
      .wet-lab-notebook h2{
 +
        margin-top: 0px;
 +
        font-size: 20pt;
 +
      }
 
       .week.visible-week{
 
       .week.visible-week{
 
         display:block;
 
         display:block;
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       .week{
 
       .week{
 
         display: none;
 
         display: none;
 +
      }
 +
      .next-week, .last-week{
 +
        color:#3377A8;
 +
        cursor: pointer;
 
       }
 
       }
 
     </style>
 
     </style>
  
  
−
     <section class="week visible-week" id="week-1">
+
     <section class="week" id="week-1">
−
       <h1>Week 1</h1>
+
       <h1>Week 1 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
        <p>
 +
          Our team was being recruited. Nothing happened this week!
 +
        </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-2">
 
     <section class="week" id="week-2">
−
       <h1>Week 2</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 2 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
        <p>
 +
          Our team was being recruited. Nothing happened this week!
 +
        </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-3">
 
     <section class="week" id="week-3">
−
       <h1>Week 3</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 3 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
        <p>
 +
          Our team was being recruited. Nothing happened this week!
 +
        </p>
 
     </section>
 
     </section>
−
     <section class="week" id="week-4">
+
     <section class="week visible-week" id="week-4">
−
       <h1>Week 4</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 4 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>01-25: Kick Off Meeting</h2>
 +
      <p>
 +
        The adventure begins!
 +
      </p>
 +
      <img src="https://static.igem.org/mediawiki/2017/5/57/T--INSA-UPS_France--Notebook_Week-4.jpg" alt="">
 
     </section>
 
     </section>
 
     <section class="week" id="week-5">
 
     <section class="week" id="week-5">
−
       <h1>Week 5</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 5 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>02-02: 1st brainstorming meeting</h2>
 +
      <p>
 +
        <i>on the track of antimicrobial peptides...</i>
 +
      </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-6">
 
     <section class="week" id="week-6">
−
       <h1>Week 6</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 6 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-7">
 
     <section class="week" id="week-7">
−
       <h1>Week 7</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 7 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>02-16: 4th brainstorming meeting</h2>
 +
      <p>
 +
        <i>Peptides safety and first device</i>
 +
      </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-8">
 
     <section class="week" id="week-8">
−
       <h1>Week 8</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 8 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-9">
 
     <section class="week" id="week-9">
−
       <h1>Week 9</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 9 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>03-02: 6th brainstorming meeting</h2>
 +
      <p>
 +
        <i>Technical issues</i>
 +
      </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-10">
 
     <section class="week" id="week-10">
−
       <h1>Week 10</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 10 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>03-09: 7th brainstorming meeting</h2>
 +
      <p><i>
 +
        Dropping of first device
 +
      </i></p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-11">
 
     <section class="week" id="week-11">
−
       <h1>Week 11</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 11 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2></h2>
 
     </section>
 
     </section>
 
     <section class="week" id="week-12">
 
     <section class="week" id="week-12">
−
       <h1>Week 12</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 12 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>03-25: Team Building</h2>
 +
      <p>
 +
        Our team met for the first time outside the INSA buildings and shared a delicious korean meal after brainstorming the whole afternoon !
 +
      </p>
 +
      <img src="https://static.igem.org/mediawiki/2017/9/9f/T--INSA-UPS_France--Notebook_Week-12_1.jpg" alt="">
 +
      <img src="https://static.igem.org/mediawiki/2017/5/54/T--INSA-UPS_France--Notebook_Week-12_2.jpg" alt="">
 
     </section>
 
     </section>
 
     <section class="week" id="week-13">
 
     <section class="week" id="week-13">
−
       <h1>Week 13</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 13 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>03-30: 10th brainstorming meeting</h2>
 +
      <p><i>
 +
        Technical solutions
 +
      </i></p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-14">
 
     <section class="week" id="week-14">
−
       <h1>Week 14</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 14 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>04-06: 12th brainstorming meeting</h2>
 +
      <p><i>
 +
        Second device proposition
 +
      </i></p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-15">
 
     <section class="week" id="week-15">
−
       <h1>Week 15</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 15 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-16">
 
     <section class="week" id="week-16">
−
       <h1>Week 16</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 16 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>04-19: Meeting with Marc Lemonnier, CEO of Antabio</h2>
 +
      <p>
 +
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a1">his testimony here.</a>
 +
      </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-17">
 
     <section class="week" id="week-17">
−
       <h1>Week 17</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 17 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-18">
 
     <section class="week" id="week-18">
−
       <h1>Week 18</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 18 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-19">
 
     <section class="week" id="week-19">
−
       <h1>Week 19</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 19 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>05-13: Paul &amp; Margaux joined our team</h2>
 +
      <img src="https://static.igem.org/mediawiki/2017/6/6a/T--INSA-UPS_France--Notebook_Week-19_1.jpg" alt="">
 
     </section>
 
     </section>
 
     <section class="week" id="week-20">
 
     <section class="week" id="week-20">
−
       <h1>Week 20</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 20 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>05-18: iGEM distribution kit received</h2>
 +
      <img src="https://static.igem.org/mediawiki/2017/7/70/T--INSA-UPS_France--Notebook_Week-20.jpg" alt="">
 
     </section>
 
     </section>
 
     <section class="week" id="week-21">
 
     <section class="week" id="week-21">
−
       <h1>Week 21</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 21 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>05-25: Parts design day</h2>
 +
      <!-- text -->
 +
      <img src="https://static.igem.org/mediawiki/2017/f/f0/T--INSA-UPS_France--Notebook_Week-21_2.jpg" alt="">
 
     </section>
 
     </section>
 
     <section class="week" id="week-22">
 
     <section class="week" id="week-22">
−
       <h1>Week 22</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 22 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>05-28: Official announcement of our subject</h2>
 +
      <p>
 +
        We officialy the subject of our project on social media!
 +
      </p>
 +
      <h2>05-31: Exposciences</h2>
 +
      <p>
 +
        We took part to the Exposciences event.
 +
        <!-- LINK -->
 +
      </p>
 
     </section>
 
     </section>
 
     <section class="week" id="week-23">
 
     <section class="week" id="week-23">
−
       <h1>Week 23</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 23 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>06-06: Meeting with the CEO of Sunwaterlife</h2>
 +
      <p>
 +
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
 +
      </p>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Taking care of <i>V. harveyi</i></h2>
 +
            <ul>
 +
              <li>Reception and storage of <i>V. harveyi</i> BB120 (WT) and <i>V. harveyi</i> JMH626 (&Delta;cqsA &Delta;luxQ &Delta;luxN) CmR at -80 &deg;C.</li>
 +
              <li>Investigation of <i>V. harveyi</i> antibiotics resistances.</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-24">
 
     <section class="week" id="week-24">
−
       <h1>Week 24</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 24 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>06-15: Scope statement start</h2>
 +
      <p><i>
 +
        Third device proposition
 +
      </i></p>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Taking charge of the lab and of <i>V. harveyi</i></h2>
 +
            <ul>
 +
              <li>Growth kinetic assay in order to determine the adequate NaCl concentration for <i>V. harveyi</i> growth. </li>
 +
              <li>Reception of IDT gBlocks and storage</li>
 +
              <li>Transformation of DH5&alpha; competent cells with pYFP, pDsRed, pPIZ&alpha;, pBR322 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-25">
 
     <section class="week" id="week-25">
−
       <h1>Week 25</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 25 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>06-19: Meeting with Doctors Without Borders, regional manager</h2>
 +
      <p>
 +
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a5">her testimony here.</a>
 +
      </p>
 +
      <h2>06-20: Cholera survey</h2>
 +
      <p>
 +
        We shared our survey about cholera on the social media.
 +
        <!-- lINK -->
 +
      </p>
 +
      <h2>06-22: Meeting with all the team members and supervisors</h2>
 +
      <p><i>
 +
        Final choice of the global device purpose
 +
      </i></p>
 +
      <h2>06-23: First appearance of Sobki, our mascott!</h2>
 +
      <img src="https://static.igem.org/mediawiki/2017/2/2a/T--INSA-UPS_France--Logo.png" alt="">
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Plasmids preparation</h2>
 +
            <ul>
 +
              <li>Transformation of DH5&alpha; competent cells with pGAP, pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
 +
              <li>Scale up of the preparative digestion, in order to have more DNA matrix.</li>
 +
              <li>Reception of the PCR primer.</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-26">
 
     <section class="week" id="week-26">
−
       <h1>Week 26</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 26 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>06-28: Meeting with the CEO of Sunwaterlife, building a collaboration</h2>
 +
      <p>
 +
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
 +
      </p>
 +
      <h2>06-30: First steps of our ethical matrix</h2>
 +
      <!-- img éthique -->
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Assembly of <i>V. harveyi</i> gene circuit : starting the sub-cloning work</h2>
 +
            <ul>
 +
              <li>Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part</li>
 +
              <li>Digestion of the PCR amplicons and ligation with the appropriate cloning vector. </li>
 +
              <li>Cloning attempt #1 and #2 to sub-clone &nbsp;Vh1, Vh2 and &nbsp;Vh3 in their respective vector.  </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-27">
 
     <section class="week" id="week-27">
−
       <h1>Week 27</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 27 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>07-07: Delft European iGEM Meetup</h2>
 +
      <!-- link meetups -->
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Assembly of <i>V. harveyi</i> gene circuit : continuing the sub-cloning work</h2>
 +
            <ul>
 +
              <li>Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map. </li>
 +
              <li>New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </section>
 +
    <section class="week" id="week-29">
 +
      <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 29 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
            <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Assembly of <i>V. harveyi</i> gene circuit : still no chance for the sub-cloning work</h2>
 +
            <ul>
 +
              <li>PCR amplification of a GFP gene for the characterization of <i>V. harveyi</i> sensing circuit.</li>
 +
              <li>Attempt #4 to sub-clone Vh1, Vh2 and  Vh3 in their respective vector but starting with fresh prepared PCR amplicons and plasmids and an alternative protocol. </li>
 +
              <li>Attempt #5 to #7  for <i>harveyi</i>’s  sub-cloning work and checking of the restriction map. </li>
 +
              <li>Meeting with the advisors to elaborate a new strategy  for the sub cloning work.</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-28">
 
     <section class="week" id="week-28">
−
       <h1>Week 28</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 28 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>07-14: Meeting with Doctors Without Borders, cholera specialist</h2>
 +
      <h2>07-21: Parisian meetup</h2>
 +
      <!-- link meetups -->
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Assembly of <i>V. harveyi</i> gene circuit : continuing the sub-cloning work</h2>
 +
            <ul>
 +
              <li>Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map. </li>
 +
              <li>New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-29">
 
     <section class="week" id="week-29">
−
       <h1>Week 29</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 29 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
            <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Assembly of <i>V. harveyi</i> gene circuit : A new strategy</h2>
 +
            <ul>
 +
              <li>Amplification of the IDT gblocks, digestion of the PCR amplicons. </li>
 +
              <li>Amplification and digestion of the sub-cloning vectors. </li>
 +
              <li>Ligation and transformation in <i>E. coli</i> STELLAR competent cells (attempt #8). </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>     
 
     </section>
 
     </section>
 
     <section class="week" id="week-30">
 
     <section class="week" id="week-30">
−
       <h1>Week 30</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 30 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>07-27: Meeting at TWB</h2>
 +
      <p>
 +
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a2">their testimony here.</a>
 +
      </p>
 +
      <h2>Experiments</h2>
 +
            <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Assembly of <i>V. harveyi</i> gene circuit : The first transformant</h2>
 +
            <ul>
 +
              <li>TA cloning (attempt #9): amplification of the IDT gblocks with KAPA2G polymerase , digestion of the PCR amplicons. Ligation with pGEM vector TA cloning vector. </li>
 +
              <li>Analysis of the transformants (attempt #8 and #9)  by checking the restriction map. </li>
 +
              <li>The part 1 and 2 were successfully cloned in pBR322 and pGEM.  </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 +
   
 
     <section class="week" id="week-31">
 
     <section class="week" id="week-31">
−
       <h1>Week 31</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 31 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
            <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Step 2 : reconstruction of CqsS Rc by ligating Vh2 to Vh1 in pBR322. </h2>
 +
            <ul>
 +
              <li>Storage of the transformants with Vh1 and Vh2 in glycerol at -80&deg;C.</li>
 +
              <li>Amplification, digestion of pBR322-Vh1,  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt A)</li>
 +
              <li>Transformation of Vh3 (attempt #10) in <i>E. coli</i> DH5&alpha; competent cells </li>
 +
              <li>Analysis of the transformants (attempt #A and #10)  by checking the restriction map.</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 +
     
 
     </section>
 
     </section>
 +
 
     <section class="week" id="week-32">
 
     <section class="week" id="week-32">
−
       <h1>Week 32</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 32 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Step 2 CqsS Rc assembly in progress…still no success for Vh3</h2>
 +
            <ul>
 +
              <li>Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt B and C)</li>
 +
              <li>Transformation of Vh3 (attempt #11 to #13) in <i>E. coli</i> TOP10 competent cells using an alternative restriction enzyme :  PstI-HF restriction enzyme is remplaced  by SpeI.</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-33">
 
     <section class="week" id="week-33">
−
       <h1>Week 33</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 33 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <h2>Step 2 CqsS Rc assembly in progress, preparation of the conjugation</h2>
 +
            <ul>
 +
              <li>Analysis of the transformants (attempt #13) &nbsp;by checking the restriction map : the part 3 is successfully cloned in pSB1C3. The transformant is stored &nbsp;in glycerol at -80&deg;C.</li>
 +
              <li>Amplification of pBBR1MCS-4 and pBBR1MCS-5 for cloning of Vh3 and for a conjugation assay. </li>
 +
              <li>Ligation of Vh3 in pBBR1MCS-4 and transformation in <i>E. coli</i> TOP10 (attempt &alpha;). </li>
 +
              <li>Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt D to F) </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-34">
 
     <section class="week" id="week-34">
−
       <h1>Week 34</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 34 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
           
 +
            <ul>
 +
              <li>Analysis of the transformants (attempt #F) &nbsp;by checking the restriction map : the part Vh1+Vh2 is successfully cloned in pBR322. The transformant is stored &nbsp;in glycerol at -80&deg;C.</li>
 +
              <li>Amplification, digestion of pBR322-Vh1+Vh2, pSB1C3-Vh3. Ligation and transformation (attempt #1 and #2) in <i>E. coli</i> TOP10 competent cells. </li>
 +
              <li>Ligation of Vh3 in pBBR1MCS-4 and transformation in <i>E. coli</i> TOP10 (attempt &beta; and &gamma;)</li>
 +
              <li>Vh3 part validation : diacetyl production assay by NMR detection with <i>E. coli</i> BL21. </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-35">
 
     <section class="week" id="week-35">
−
       <h1>Week 35</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 35 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <ul>
 +
              <li>2nd  diacetyl production assay by NMR detection with <i>E. coli</i> BL21.</li>
 +
              <li>Success of the conjugation assay of pBBR1MCS-4. </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-36">
 
     <section class="week" id="week-36">
−
       <h1>Week 36</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 36 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>09-04: Start of our Ulule campaign</h2>
 +
      <p>
 +
        We started a crowdfunding campaign on Ulule, a french platform. <!-- link -->
 +
      </p>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <ul>
 +
              <li>3rd  Diacetyl production assay by NMR detection with <i>E. coli</i> MG1655. </li>
 +
              <li>Amplification of pBBR1MCS-4 and pBBR1MCS-5. </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-37">
 
     <section class="week" id="week-37">
−
       <h1>Week 37</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 37 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <ul>
 +
              <li>4th diacetyl production assay by NMR detection with <i>E. coli</i> MG1655 in M9 media supplemented with xylose and pyruvate. </li>
 +
              <li>Transformation of <i>E. coli</i> TOP10 competent cells with GFP from iGEM kit</li>
 +
              <li>Ligation and transformation of pBBR1MCS-4 to RFP from iGEM kit in <i>E. coli</i> TOP10 competent cells for conjugation to <i>V. harveyi</i>. </li>
 +
              <li>Preparation of the clones for sequencing. </li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-38">
 
     <section class="week" id="week-38">
−
       <h1>Week 38</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 38 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>09-22: Meeting with UNICEF</h2>
 +
      <p>
 +
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a6">Alama Keita's testimony here.</a>
 +
      </p>
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <ul>
 +
              <li>Results of the 4th Diacetyl production assay by NMR detection: a small peak of diacetyl is visible. </li>
 +
              <li>5th diacetyl production assay by NMR detection with <i>E. coli</i> MG1655 in M9 media supplemented with xylose and pyruvate. </li>
 +
              <li>Results of ligation of RFP in conjugative plasmid: all transformants are red. Two transformants are stored at -80&deg;C in order to be used for conjugation assay. </li>
 +
              <li>Preparative work for the ligation of Vh3 in pBBR1MCS-4 with fresh material</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-39">
 
     <section class="week" id="week-39">
−
       <h1>Week 39</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 39 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      <h2>09-29: European Researcher's Night</h2>
 +
      <!-- link -->
 +
      <h2>Experiments</h2>
 +
      <div class="wet-lab-notebook">
 +
        <div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 +
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 +
        </div>
 +
        <div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
 +
            </p>-->
 +
          </div>
 +
          <div>
 +
            <ul>
 +
              <li>Observation by microscopy of <i>V. harveyi</i> (rfp) transformant</li>
 +
              <li>Ligation of pBBR1MCS-4 and Vh3 at 16&deg;C overnight, and transformation in competent <i>E. coli</i> (Stellars)</li>
 +
            </ul>
 +
          </div>
 +
          <div>
 +
            <!--<h2>Titre</h2>
 +
            <p>
 +
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
            </p>-->
 +
          </div>
 +
        </div>
 +
      </div>
 
     </section>
 
     </section>
 
     <section class="week" id="week-40">
 
     <section class="week" id="week-40">
−
       <h1>Week 40</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 40 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-41">
 
     <section class="week" id="week-41">
−
       <h1>Week 41</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 41 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
     <section class="week" id="week-42">
 
     <section class="week" id="week-42">
−
       <h1>Week 42</h1>
+
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 42 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
     </section>
 
     </section>
 
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Revision as of 16:51, 3 October 2017

Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Notebook

Here is a summary of what we did for our project, week by week. You can go on our Human Practices labbook page to know more about how each event affected us to take a decision about the future of our project.

January

1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31 1 2 3 4

February

29 30 31 1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 1 2 3 4

March

26 27 28 1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31 1

April

26 27 28 29 30 31 1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 1 2 3 4 5 6

May

30 1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31 1 2 3

June

28 29 30 31 1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 1

July

25 26 27 28 29 30 1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31 1 2 3 4 5

August

30 31 1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31 1 2

September

27 28 29 30 31 1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

October

1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31 1 2 3 4

Week 1

Our team was being recruited. Nothing happened this week!

Week 2

Our team was being recruited. Nothing happened this week!

Week 3

Our team was being recruited. Nothing happened this week!

Week 4

01-25: Kick Off Meeting

The adventure begins!

Week 5

02-02: 1st brainstorming meeting

on the track of antimicrobial peptides...

Week 6

Week 7

02-16: 4th brainstorming meeting

Peptides safety and first device

Week 8

Week 9

03-02: 6th brainstorming meeting

Technical issues

Week 10

03-09: 7th brainstorming meeting

Dropping of first device

Week 11

Week 12

03-25: Team Building

Our team met for the first time outside the INSA buildings and shared a delicious korean meal after brainstorming the whole afternoon !

Week 13

03-30: 10th brainstorming meeting

Technical solutions

Week 14

04-06: 12th brainstorming meeting

Second device proposition

Week 15

Week 16

04-19: Meeting with Marc Lemonnier, CEO of Antabio

You can read the result of his testimony here.

Week 17

Week 18

Week 19

05-13: Paul & Margaux joined our team

Week 20

05-18: iGEM distribution kit received

Week 21

05-25: Parts design day

Week 22

05-28: Official announcement of our subject

We officialy the subject of our project on social media!

05-31: Exposciences

We took part to the Exposciences event.

Week 23

06-06: Meeting with the CEO of Sunwaterlife

You can read the result of his testimony here.

Experiments

Taking care of V. harveyi

  • Reception and storage of V. harveyi BB120 (WT) and V. harveyi JMH626 (ΔcqsA ΔluxQ ΔluxN) CmR at -80 °C.
  • Investigation of V. harveyi antibiotics resistances.

Week 24

06-15: Scope statement start

Third device proposition

Experiments

Taking charge of the lab and of V. harveyi

  • Growth kinetic assay in order to determine the adequate NaCl concentration for V. harveyi growth.
  • Reception of IDT gBlocks and storage
  • Transformation of DH5α competent cells with pYFP, pDsRed, pPIZα, pBR322 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.

Week 25

06-19: Meeting with Doctors Without Borders, regional manager

You can read the result of her testimony here.

06-20: Cholera survey

We shared our survey about cholera on the social media.

06-22: Meeting with all the team members and supervisors

Final choice of the global device purpose

06-23: First appearance of Sobki, our mascott!

Experiments

Plasmids preparation

  • Transformation of DH5α competent cells with pGAP, pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.
  • Scale up of the preparative digestion, in order to have more DNA matrix.
  • Reception of the PCR primer.

Week 26

06-28: Meeting with the CEO of Sunwaterlife, building a collaboration

You can read the result of his testimony here.

06-30: First steps of our ethical matrix

Experiments

Assembly of V. harveyi gene circuit : starting the sub-cloning work

  • Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part
  • Digestion of the PCR amplicons and ligation with the appropriate cloning vector.
  • Cloning attempt #1 and #2 to sub-clone  Vh1, Vh2 and  Vh3 in their respective vector.  

Week 27

07-07: Delft European iGEM Meetup

Experiments

Assembly of V. harveyi gene circuit : continuing the sub-cloning work

  • Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map.
  • New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  

Week 29

Experiments

Assembly of V. harveyi gene circuit : still no chance for the sub-cloning work

  • PCR amplification of a GFP gene for the characterization of V. harveyi sensing circuit.
  • Attempt #4 to sub-clone Vh1, Vh2 and  Vh3 in their respective vector but starting with fresh prepared PCR amplicons and plasmids and an alternative protocol.
  • Attempt #5 to #7  for harveyi’s  sub-cloning work and checking of the restriction map.
  • Meeting with the advisors to elaborate a new strategy  for the sub cloning work.

Week 28

07-14: Meeting with Doctors Without Borders, cholera specialist

07-21: Parisian meetup

Experiments

Assembly of V. harveyi gene circuit : continuing the sub-cloning work

  • Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map.
  • New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  

Week 29

Experiments

Assembly of V. harveyi gene circuit : A new strategy

  • Amplification of the IDT gblocks, digestion of the PCR amplicons.
  • Amplification and digestion of the sub-cloning vectors.
  • Ligation and transformation in E. coli STELLAR competent cells (attempt #8).

Week 30

07-27: Meeting at TWB

You can read the result of their testimony here.

Experiments

Assembly of V. harveyi gene circuit : The first transformant

  • TA cloning (attempt #9): amplification of the IDT gblocks with KAPA2G polymerase , digestion of the PCR amplicons. Ligation with pGEM vector TA cloning vector.
  • Analysis of the transformants (attempt #8 and #9)  by checking the restriction map.
  • The part 1 and 2 were successfully cloned in pBR322 and pGEM.  

Week 31

Experiments

Step 2 : reconstruction of CqsS Rc by ligating Vh2 to Vh1 in pBR322.

  • Storage of the transformants with Vh1 and Vh2 in glycerol at -80°C.
  • Amplification, digestion of pBR322-Vh1,  pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt A)
  • Transformation of Vh3 (attempt #10) in E. coli DH5α competent cells
  • Analysis of the transformants (attempt #A and #10)  by checking the restriction map.

Week 32

Experiments

Step 2 CqsS Rc assembly in progress…still no success for Vh3

  • Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt B and C)
  • Transformation of Vh3 (attempt #11 to #13) in E. coli TOP10 competent cells using an alternative restriction enzyme :  PstI-HF restriction enzyme is remplaced  by SpeI.

Week 33

Experiments

Step 2 CqsS Rc assembly in progress, preparation of the conjugation

  • Analysis of the transformants (attempt #13)  by checking the restriction map : the part 3 is successfully cloned in pSB1C3. The transformant is stored  in glycerol at -80°C.
  • Amplification of pBBR1MCS-4 and pBBR1MCS-5 for cloning of Vh3 and for a conjugation assay.
  • Ligation of Vh3 in pBBR1MCS-4 and transformation in E. coli TOP10 (attempt α).
  • Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt D to F)

Week 34

Experiments

  • Analysis of the transformants (attempt #F)  by checking the restriction map : the part Vh1+Vh2 is successfully cloned in pBR322. The transformant is stored  in glycerol at -80°C.
  • Amplification, digestion of pBR322-Vh1+Vh2, pSB1C3-Vh3. Ligation and transformation (attempt #1 and #2) in E. coli TOP10 competent cells.
  • Ligation of Vh3 in pBBR1MCS-4 and transformation in E. coli TOP10 (attempt β and γ)
  • Vh3 part validation : diacetyl production assay by NMR detection with E. coli BL21.

Week 35

Experiments

  • 2nd  diacetyl production assay by NMR detection with E. coli BL21.
  • Success of the conjugation assay of pBBR1MCS-4.

Week 36

09-04: Start of our Ulule campaign

We started a crowdfunding campaign on Ulule, a french platform.

Experiments

  • 3rd  Diacetyl production assay by NMR detection with E. coli MG1655.
  • Amplification of pBBR1MCS-4 and pBBR1MCS-5.

Week 37

Experiments

  • 4th diacetyl production assay by NMR detection with E. coli MG1655 in M9 media supplemented with xylose and pyruvate.
  • Transformation of E. coli TOP10 competent cells with GFP from iGEM kit
  • Ligation and transformation of pBBR1MCS-4 to RFP from iGEM kit in E. coli TOP10 competent cells for conjugation to V. harveyi.
  • Preparation of the clones for sequencing.

Week 38

09-22: Meeting with UNICEF

You can read the result of Alama Keita's testimony here.

Experiments

  • Results of the 4th Diacetyl production assay by NMR detection: a small peak of diacetyl is visible.
  • 5th diacetyl production assay by NMR detection with E. coli MG1655 in M9 media supplemented with xylose and pyruvate.
  • Results of ligation of RFP in conjugative plasmid: all transformants are red. Two transformants are stored at -80°C in order to be used for conjugation assay.
  • Preparative work for the ligation of Vh3 in pBBR1MCS-4 with fresh material

Week 39

09-29: European Researcher's Night

Experiments

  • Observation by microscopy of V. harveyi (rfp) transformant
  • Ligation of pBBR1MCS-4 and Vh3 at 16°C overnight, and transformation in competent E. coli (Stellars)

Week 40

Week 41

Week 42

Week 43

Week 44