Difference between revisions of "Team:Munich/Description"

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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<ul>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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</ul>
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<tr><td height="50" bgcolor=#ffffff></td><td class="no-padding" bgcolor=#ffffff height="32" width="960" align=center>
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<div class="dropdown">
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  <a href="/Team:Munich"><button class="dropbtnCascAID dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></button></a>
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</div><div class="dropdown">
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  <a href="project"><button class="dropbtnProject dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Project</b></p></button></a>  <div class="dropdown-contentDescription dropdown-content">
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    <a href="description"><p><b>Description</b></p></a>
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  </div>
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  <div class="dropdown-contentDesign dropdown-content">
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    <a href="design"><p><b>Design</b></p></a>
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  <div class="dropdown-contentProofofConcept dropdown-content">
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    <a href="proofofconcept"><p><b>Proof of Concept</b></p></a>
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  </div>
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  <div class="dropdown-contentDemonstration dropdown-content">
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    <a href="demonstration"><p><b>Demonstration</b></p></a>
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  <div class="dropdown-contentApplication dropdown-content">
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    <a href="application"><p><b>Application</b></p></a>
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  </div>
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  <div class="dropdown-contentFinalResults dropdown-content">
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    <a href="finalresults"><p><b>Final Results</b></p></a>
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  <div class="dropdown-contentEntrepreneurship dropdown-content">
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    <a href="entrepreneurship"><p><b>Entrepeneurship</b></p></a>
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  <div class="dropdown-contentAchievements dropdown-content">
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    <a href="achievements"><p><b>Achievements</b></p></a>
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</div><div class="dropdown" valign=center>
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<a href="wetlab"><button class="dropbtnWetlab dropbtn" valign=center><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Wetlab</b></p></button></a>
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  <div class="dropdown-contentProtocolsMethods dropdown-content">
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    <a href="protocolsmethods"><p><b>Protocols and<br> Methods</b></p></a>
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    <a href="materials"><p><b>Materials</b></p></a>
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    <a href="labjournal"><p><b>LabJournal</b></p></a>
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    <a href="safety"><p><b>Safety</b></p></a>
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  <div class="dropdown-contentParts dropdown-content">
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    <a href="parts"><p><b>Parts</b></p></a>
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  <div class="dropdown-contentInterlab dropdown-content">
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    <a href="interlab"><p><b>Interlab</b></p></a>
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</div><div class="dropdown">
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  <a href="model"><button class="dropbtnModel dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Modelling</b></p></button></a></div><div class="dropdown">
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  <a href="softhardware"><button class="dropbtnSoftHardware dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Software/Hardware</b></p></button></a>
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    <a href="software"><p><b>Software</b></p></a>
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    <a href="hardware"><p><b>Hardware</b></p></a>
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  <a href="hp"><button class="dropbtnHP dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Human Practices</b></p></button></a>
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    <a href="hpsilver"><p><b>Human Practices (Silver)</b></p></a>
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    <a href="ihpgold"><p><b>Human Practices (Gold)</b></p></a>
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    <a href="publicengagement"><p><b>Public Engagement</b></p></a>
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  <div class="dropdown-contentCollaborations dropdown-content">
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    <a href="collaborations"><p><b>Collaborations</b></p></a>
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  <a href="/Team:Munich/Team"><button class="dropbtnTeam dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Team</b></p></button></a>
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    <a href="/Team:Munich/Team"><p><b>Team members</b></p></a>
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    <a href="supervisors"><p><b>Supervisors</b></p></a>
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    <a href="supervisors"><p><b>PIs</b></p></a>
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    <a href="supervisors"><p><b>Institutions</b></p></a>
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    <a href="sponsors"><p><b>Sponsor
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s</b></p></a>
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  <div class="dropdown-contentAttributions dropdown-content">
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    <a href="/Team:Munich/Team"><p><b>Attributions</b></p></a>
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<p class="introduction">
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Thanks to advances in molecular biology and biochemistry, scientist have been able to consistently detect lower and lower concentration of molecules, to the point that, to date, single molecules can be reliably recognised with methods such as polymerase chain reaction (PCR) or fluorescence in situ hybridization (FISH). This has opened doors for better and more accurate diagnostic tests that biomarkers like nucleic acids and proteins as targets. Through such advances, the field of molecular diagnostics developed. Unfortunately, current standard methods require expensive equipment or trained personnel, which generally limits their usability to hospitals or laboratories. Only in recent times has there been a push to develop new tests that fuse the reliability of standard methods with affordable platforms such as gene chips or paper strips. We wanted to help close this gap and set out to engineer a diagnosis principle for the detection of a wide array of targets that could be used without difficult technical requirements.
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<h5>Advice on writing your Project Description</h5>
 
  
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<p>
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<tr><td colspan=3 align=center valign=center>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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<h3>Molecular cloning</h3>
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<p>
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Our project, which we dubbed Cas13a controlled assay for infectious diseases (CascAID) features the recently identified CRISPR/Cas effector Cas13a. Unlike other proteins in the familiy, Cas13a has the unique ability to bind and cleave specific RNA targets rather than DNA ones. Moreover after cleaving its target, Cas13a presents collateral activity i.e. it can unspecifically cleave RNA molecules. By using Cas13a, our system can detect virtually detect any RNA target. This is done by changing the crRNA in the protein, that is a short RNA sequence that determines what is recognized as target.  
 
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<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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<h5>Inspiration</h5>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<ul>
 
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
 
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
 
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</ul>
 
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Revision as of 14:13, 4 October 2017

Description

Design

Proof of Concept

Demonstration

Application

Final Results

Entrepeneurship

Achievements

Protocols and
Methods

Materials

LabJournal

Safety

Parts

Interlab

Software

Hardware

Human Practices (Silver)

Human Practices (Gold)

Public Engagement

Collaborations

Team members

Supervisors

PIs

Institutions

Sponsor s

Attributions

Attributions

Thanks to advances in molecular biology and biochemistry, scientist have been able to consistently detect lower and lower concentration of molecules, to the point that, to date, single molecules can be reliably recognised with methods such as polymerase chain reaction (PCR) or fluorescence in situ hybridization (FISH). This has opened doors for better and more accurate diagnostic tests that biomarkers like nucleic acids and proteins as targets. Through such advances, the field of molecular diagnostics developed. Unfortunately, current standard methods require expensive equipment or trained personnel, which generally limits their usability to hospitals or laboratories. Only in recent times has there been a push to develop new tests that fuse the reliability of standard methods with affordable platforms such as gene chips or paper strips. We wanted to help close this gap and set out to engineer a diagnosis principle for the detection of a wide array of targets that could be used without difficult technical requirements.

Molecular cloning

Our project, which we dubbed Cas13a controlled assay for infectious diseases (CascAID) features the recently identified CRISPR/Cas effector Cas13a. Unlike other proteins in the familiy, Cas13a has the unique ability to bind and cleave specific RNA targets rather than DNA ones. Moreover after cleaving its target, Cas13a presents collateral activity i.e. it can unspecifically cleave RNA molecules. By using Cas13a, our system can detect virtually detect any RNA target. This is done by changing the crRNA in the protein, that is a short RNA sequence that determines what is recognized as target.