Difference between revisions of "Team:Cardiff Wales/basicparts"

Line 25: Line 25:
 
     <td>TSH</td>
 
     <td>TSH</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td>A protein coding sequence of a gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does not</b> have His-tags for purification </td>
+
     <td>A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does not</b> have His-tags for purification </td>
−
<td>~length</td>
+
<td>780bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 32: Line 32:
 
     <td>TSHH</td>
 
     <td>TSHH</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td> A protein coding sequence of a gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does</b> have His-tags for purification</td>
+
     <td> A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does</b> have His-tags for purification</td>
−
<td>~length</td>
+
<td>813bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 40: Line 40:
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
 
     <td>An inducible promoter that responds to the presence of jasmonic acid in the cell</td>
 
     <td>An inducible promoter that responds to the presence of jasmonic acid in the cell</td>
−
<td>~length</td>
+
<td>500bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 47: Line 47:
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
 
     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
 
     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
−
<td>~length</td>
+
<td>497bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 54: Line 54:
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
 
     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
 
     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
−
<td>~length</td>
+
<td>506bp</td>
 
   </tr>
 
   </tr>
 
<tr>
 
<tr>
Line 60: Line 60:
 
     <td>WRKY30</td>
 
     <td>WRKY30</td>
 
     <td>pSB1C3</td>
 
     <td>pSB1C3</td>
−
     <td>An inducible promoter that responds to the presence of cellulose-derived oligomers in the cell</td>
+
     <td>An inducible promoter that responds to the presence of cellulose-derived oligomers</td>
−
<td>~length</td>
+
<td>485bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 69: Line 69:
 
     <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate,
 
     <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate,
 
  luciferin, which can be used to quantify gene expression </td>
 
  luciferin, which can be used to quantify gene expression </td>
−
<td>~length</td>
+
<td>1653bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>

Revision as of 10:07, 6 October 2017




Basic Parts






Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.




Registry link Part Plasmid Summary Approximate Length
BBa_K2404000 TSH pSB1C3 A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification 780bp
BBa_K2404001 TSHH pSB1C3 A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification 813bp
BBa_K2404002 PDF1.2 pSB1C3 An inducible promoter that responds to the presence of jasmonic acid in the cell 500bp
BBa_K2404003 PR2 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell 497bp
BBa_K2404004 GST6 pSB1C3 An inducible promoter that responds to the presence of salicylic acid in the cell 506bp
BBa_K2404005 WRKY30 pSB1C3 An inducible promoter that responds to the presence of cellulose-derived oligomers 485bp
BBa_K2404006 Luc+ pSB1C3 A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression 1653bp
BBa_K2404007 PR2-PDF1.2 pSB1C3 A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the jasmonic acid responsive PDF1.2 at the most 3' end ~length
BBa_K2404008 PR2-GST6 pSB1C3 A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end ~length
BBa_K2404009 PDF1.2-GST6 pSB1C3 A composite promoter of both the jasmonic acid responsive PDF1.2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end ~length
BBa_P10401 NosT pSB1C3 A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 ~length
BBa_P10000 LexA pSB1C3 An estradiol inducible promoter from the LexA gene which is native to Escherichia coli ~length
BBa_P10003 35S-OTMV pSB1C3 A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus ~length