Difference between revisions of "Team:BNU-China/Description"

Line 60: Line 60:
 
         </ul>
 
         </ul>
 
       </div>
 
       </div>
 +
        <div id="w_text">
 +
        <h1 id="title1">Background</h1>
 +
        <p style="text-indent:0px;">
 +
          This year our project focuses on improving the yeast surface display system.Cell-surface display systems have been successfully developed in various microorganisms, such as Escherichia coli. While, yeast is one of the most suitable host strains for this arming technology, because of its rigid cell walls (around 110–200 nm wild) and useful platform for protein production which allows the folding and glycosylation of expressed heterologous eukaryotic proteins.  The rigid yeast cell wall was mainly constituted by cross-linked β-1, 3/1, 6-glucans, mannoproteins, and chitin. Among these components, the β-1,6-glucan，though takes up a little quantities of the system, plays an essential role in anchoring cell-wall proteins. Another important part in the display system is glucanase-extractable mannoproteins which contains glycosylphosphatidylinositol (GPI) anchors, such as agglutinin (Aga1 and Aga2). These GPI-anchored proteins can be utilized in anchoring foreign proteins by genetic engineering. The specific function of GPI-anchored proteins is described in later section.
 +
        </p>
 +
        <img src="images/HP/图1.JPG" alt="Sorry, the image is not spupported by your browser.">
 +
        <h4>(Figure 1 Architecture of the yeast cell wall. SMP, glucanase-extractable<br>
 +
              surface-layer mannoprotein; PP, SDS-extractable periplasmic protein. <br>
 +
                (Seiji SHIBASAKI, et al. Analytical Science, 2009.)<br>
 +
            )</h4>
 +
 +
 +
 +
 +
 +
 
   </div>
 
   </div>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 03:12, 7 October 2017

BNU-China ">

Background

This year our project focuses on improving the yeast surface display system.Cell-surface display systems have been successfully developed in various microorganisms, such as Escherichia coli. While, yeast is one of the most suitable host strains for this arming technology, because of its rigid cell walls (around 110–200 nm wild) and useful platform for protein production which allows the folding and glycosylation of expressed heterologous eukaryotic proteins. The rigid yeast cell wall was mainly constituted by cross-linked β-1, 3/1, 6-glucans, mannoproteins, and chitin. Among these components, the β-1,6-glucan，though takes up a little quantities of the system, plays an essential role in anchoring cell-wall proteins. Another important part in the display system is glucanase-extractable mannoproteins which contains glycosylphosphatidylinositol (GPI) anchors, such as agglutinin (Aga1 and Aga2). These GPI-anchored proteins can be utilized in anchoring foreign proteins by genetic engineering. The specific function of GPI-anchored proteins is described in later section.

Sorry, the image is not spupported by your browser.

(Figure 1 Architecture of the yeast cell wall. SMP, glucanase-extractable
surface-layer mannoprotein; PP, SDS-extractable periplasmic protein.
(Seiji SHIBASAKI, et al. Analytical Science, 2009.)
)