Difference between revisions of "Team:Amazonas Brazil/Software"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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/***************************************************** DEFAULT WIKI SETTINGS ****************************************************/
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<h1>Software</h1>
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<h3>Best Software Tool Special Prize</h3>
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#home_logo, #sideMenu { display:none; } #sideMenu, #top_title, .patrollink {display:none;} #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;margin-right: 0;} body {background-color:white; } #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
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<p>Regardless of the topic, iGEM projects often create or adapt computational tools to move the project forward. Because they are born out of a direct practical need, these software tools (or new computational methods) can be surprisingly useful for other teams. Without necessarily being big or complex, they can make the crucial difference to a project's success. This award tries to find and honor such "nuggets" of computational work.
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS?action=raw&ctype=text/css">
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Software Tool prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS:icon?action=raw&ctype=text/css">
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You must also delete the message box on the top of this page to be eligible for this prize.
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<title>Wiki_iGEM_Amazonas</title>
  
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<h5> Inspiration </h5>
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<p>
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Here are a few examples from previous teams:
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<ul>
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<li><a href="https://2016.igem.org/Team:BostonU_HW">2016 BostonU HW</a></li>
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<li><a href="https://2016.igem.org/Team:Valencia_UPV">2016 Valencia UPV</a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Software">2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#Software">2014 Aachen</a></li>
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        <li><a class="Link-pg" style="padding: 0 1em;" href="#home"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png" width="104.04" height="90"style="margin-top: 0.3em;"></a></li>
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              <a href="https://twitter.com/IGEM_Amazonas" class="fa-icon1" title="" style="padding-right: 0;color: #fff;">
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                  <i class="fa fa-twitter" aria-hidden="true"></i>
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                <h1 align="middle" style="font-size: 4em;">Software</h1>
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                <h2 align="middle" style="font-size: 2.5em;">A platform to expand the iGEM Experience</h2>
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<h1>CRISPeasy</h1>
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            <h3>Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
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              </h3>
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              <h4 style="padding: 20px 0 0 0">What's the boost?</h4>
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              <div style="padding: 20px 0 0 0;  text-align: justify;
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              text-justify: inter-word;">
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              <p>Scientific community have been achieved great results using CRISPR-Cas9 mediated
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              genome editing techniques. While these methods are moving forward, some methodological
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                aspects related to this technique could be further improved to bring up this science
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                revolution even more.</p>
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              <p>In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite
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                laborious days of work in lab due to the multi-plasmid based edition. The system
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                não é padronizado e, em determinadas metodologias, demands more than one plasmid,
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                each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consist
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              in the addition of a linear DNA carrying homologous arms to provide a donor
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                DNA to insert information into the genome during the HDR repair via. However,
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              the linear donor DNA system has low edition efficiency due the action of DNA restriction
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                systems present in wild type and E. coli strains, which can degrade the linear DNAs
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              before integration into genome. All this demands plenty of time and a lot of wet lab
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                hours of work. To verify this information, we got in touch with other iGEM's teams who
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                had work with CRISPR/Cas9 in the previous years, you can check this outreach part with
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                more details here.</p>
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Revision as of 20:03, 8 October 2017

Wiki_iGEM_Amazonas

Software

A platform to expand the iGEM Experience

CRISPeasy

Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.

What's the boost?

Scientific community have been achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some methodological aspects related to this technique could be further improved to bring up this science revolution even more.

In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite laborious days of work in lab due to the multi-plasmid based edition. The system não é padronizado e, em determinadas metodologias, demands more than one plasmid, each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consist in the addition of a linear DNA carrying homologous arms to provide a donor DNA to insert information into the genome during the HDR repair via. However, the linear donor DNA system has low edition efficiency due the action of DNA restriction systems present in wild type and E. coli strains, which can degrade the linear DNAs before integration into genome. All this demands plenty of time and a lot of wet lab hours of work. To verify this information, we got in touch with other iGEM's teams who had work with CRISPR/Cas9 in the previous years, you can check this outreach part with more details here.