Difference between revisions of "Team:Amazonas Brazil/Software"

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                <h1 align="middle" style="font-size: 3em; margin: -1em 0 0;padding: 0;">Software</h1>
 
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                <h2 align="middle" style="font-size: 1.5em; margin: 0;padding: 0;">A platform to expand the iGEM Experience</h2>
 
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<h1>CRISPeasy</h1>
 
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            <h3>Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
 
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              <h4 style="padding: 20px 0 0 0">What's the boost?</h4>
 
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              text-justify: inter-word;">
 
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              <p>Scientific community have been achieved great results using CRISPR-Cas9 mediated
 
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              genome editing techniques. While these methods are moving forward, some methodological
 
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                aspects related to this technique could be further improved to bring up this science
 
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                revolution even more.</p>
 
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              <p>In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite
 
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                laborious days of work in lab due to the multi-plasmid based edition. The system
 
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                não é padronizado e, em determinadas metodologias, demands more than one plasmid,
 
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                each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consist
 
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              in the addition of a linear DNA carrying homologous arms to provide a donor
 
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                DNA to insert information into the genome during the HDR repair via. However,
 
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              the linear donor DNA system has low edition efficiency due the action of DNA restriction
 
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                systems present in wild type and E. coli strains, which can degrade the linear DNAs
 
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              before integration into genome. All this demands plenty of time and a lot of wet lab
 
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                hours of work. To verify this information, we got in touch with other iGEM's teams who
 
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                had work with CRISPR/Cas9 in the previous years, you can check this outreach part with
 
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                more details here.</p>
 
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Revision as of 06:12, 11 October 2017

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