Difference between revisions of "Team:ZJU-China/Parts"

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{{ZJU-China}}
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<h1>Parts</h1>
 
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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                  <!-- <h1><a class="navbar-brand" href="index.html">MuMei Lab</a></h1> -->
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            <!-- <h1><a class="navbar-brand" href="index.html">My Design</a></h1> -->
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              <li class="m_nav_item dropdown">
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                <a href="#" class="dropdown-toggle link " data-toggle="dropdown">Overview<b class="caret"></b></a>
 +
                  <ul class="dropdown-menu ">
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/overview">Project Description</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/overview/achievements">Achievements</a></li>
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                <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a>
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                  <ul class="dropdown-menu ">
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Project">Project Home</a></li>
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                  <!-- <li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Project/st">Signal Transduction</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Project/ms">Mat Synthesis</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/Project/conclusion">Conclusion</a></li> -->
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Project/improvement">Improvement</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">Interlab</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li>
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                  </ul>
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              <li class="m_nav_item dropdown">
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                <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Modelling<b class="caret"></b></a>
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                  <ul class="dropdown-menu ">
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>
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                  <li><a href="https://2017.igem.org/Team:ZJU-China/Model/coculture">Coculture</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/Model/voc">VOC analysis</a></li>
 +
                  </ul>
 +
              </li>
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 +
              <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Hardware">Hardware</a></li>
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 +
             
 +
              <li class="m_nav_item dropdown">
 +
                <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Safety<b class="caret"></b></a>
 +
                  <ul class="dropdown-menu ">
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/Safety">Environment</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/Safety/lab">Laboratory</a></li>
 +
                  </ul>
 +
              </li>
 +
 
 +
              <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Collaborations">Collaborations</a></li>
 +
 
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              <li class="m_nav_item dropdown">
 +
                <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Human Practice<b class="caret"></b></a>
 +
                  <ul class="dropdown-menu ">
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/HP">Summary</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold</a></li>
 +
                  </ul>
 +
              </li>
 +
 
 +
              <li class="m_nav_item dropdown">
 +
                <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Team<b class="caret"></b></a>
 +
                  <ul class="dropdown-menu ">
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/team">Teammates</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:ZJU-China/team/attribution">Attribution</a></li>
 +
                  </ul>
 +
              </li>
 +
 
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            </ul>
 +
          </div>
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      </nav> 
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    </div> 
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    </div>
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<p></p>
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<div style="margin-top:0;padding-top:0">
 +
  <img src="https://static.igem.org/mediawiki/2017/b/be/ZJUChina_interlab_banner.jpg" width="100%">
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  <!-- <img src="images/HP_temp/banner.jpg" width="100%"> -->
 
</div>
 
</div>
  
  
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<div style="background-color: #FFFAF0;width: 100%" class="container zjuContent">
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    <div class="col-md-9" role="main">
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      <div class="bs-docs-section">
  
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  <h1 id="introduction" class="page-header ArticleHead GreenAH">Parts<hr></h1>
 +
  <p  class="PP">Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.</p>
 +
  <p class="PP">We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.</p>
  
  
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<div class="panel panel-default">
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<p>The information needed to initially create a part on the Registry is:</p>
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  <!-- Default panel contents -->
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<ul>
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  <div class="panel-heading">Parts</div>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
  <!-- Table -->
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
  <table id="partTable" class="table">
 +
  <tr>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Designer</th>
 +
<th>Length(bp)</th>
 +
<th>Submitted</th>
  
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207000</th>
 +
    <td>Device</td>
 +
    <td>H3-RP27 expression system</td>
 +
    <td>Qianjin Jiang</td>
 +
    <td>2644</td>
 +
    <td> </td>
 +
</tr>
 +
      <th>BBa_K2207001</th>
 +
      <td></td>
 +
      <td>Hex promoter</td>
 +
      <td>Junbo Yang</td>
 +
      <td></td>
 +
      <td></td>
 +
 +
<tr>
 +
    <th>BBa_K2207002</th>
 +
    <td></td>
 +
    <td>Ech42 promoter</td>
 +
    <td>Junbo Yang</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207003</th>
 +
    <td></td>
 +
    <td>Trichoderma HR System I SOD-Tubulin dobule promoter</td>
 +
    <td>Yihe Zhang</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207004</th>
 +
    <td></td>
 +
    <td>Trichoderma HR System II Ech42-H3 double promoter</td>
 +
    <td>Yihe Zhang</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207005</th>
 +
    <td></td>
 +
    <td>Trichoderma HR System III L1-ADH1-RP27-L2</td>
 +
    <td>Yihe Zhang</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207006</th>
 +
    <td></td>
 +
    <td>Trichoderma HR System IV L3-ADH1-RP27-L4</td>
 +
    <td>Yihe Zhang</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207007</th>
 +
    <td></td>
 +
    <td>Trichoderma HR System V Homologous Binding SiteA</td>
 +
    <td>Yihe Zhang</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207008</th>
 +
    <td></td>
 +
    <td>Trichoderma HR System VI Homologous Binding SiteB</td>
 +
    <td>Yihe Zhang</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207009</th>
 +
    <td>Coding Sequences</td>
 +
    <td>PhlF transcription factor</td>
 +
    <td>Qianjin Jiang</td>
 +
    <td>627</td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207010</th>
 +
    <td></td>
 +
    <td>Phl Operator</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>823</td>
 +
    <td></td>
 +
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207011</th>
 +
    <td></td>
 +
    <td>Phl Operator(BBa_K2207010) mRFP</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207012</th>
 +
    <td>Composite Parts</td>
 +
    <td>DAPG sensor(PhlOF)</td>
 +
    <td> Qianjin Jiang</td>
 +
    <td>1450</td>
 +
    <td></td>
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207013</th>
 +
    <td></td>
 +
    <td>2,4-DAPG PhlABCD Cluster</td>
 +
    <td>Junmin Qian</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207014</th>
 +
    <td></td>
 +
    <td>PhlAC enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
 +
<tr>
 +
    <th>BBa_K2207015</th>
 +
    <td></td>
 +
    <td>PhlBD enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207016</th>
 +
    <td></td>
 +
    <td>PhlAB enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207017</th>
 +
    <td></td>
 +
    <td>PhlCD enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207018</th>
 +
    <td></td>
 +
    <td>GPD-CYC1 expression system</td>
 +
    <td>Shisheng Li</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207019</th>
 +
    <td></td>
 +
    <td>TRPV1-Ferritin</td>
 +
    <td>Cheng Shen</td>
 +
    <td></td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207020</th>
 +
    <td></td>
 +
    <td>Ferritin</td>
 +
    <td>Cheng Shen</td>
 +
    <td></td>
 +
    <td></td>
 +
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207021</th>
 +
    <td></td>
 +
    <td>Calcineurin-dependent response element (CDRE)</td>
 +
    <td>Shisheng Li</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207022</th>
 +
    <td></td>
 +
    <td>CDRE(BBa_K2207021) mrfp</td>
 +
    <td> Shisheng Li</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207023</th>
 +
    <td></td>
 +
    <td>Mature serine protein from Paecilomyces lilacinus</td>
 +
    <td>Zifan Xie</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207024</th>
 +
    <td></td>
 +
    <td>T7-mutant1</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207025</th>
 +
    <td></td>
 +
    <td>T7-mutant2</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207026</th>
 +
    <td></td>
 +
    <td>T7-mutant3</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207027</th>
 +
    <td></td>
 +
    <td>T7-mutant4</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207028</th>
 +
    <td></td>
 +
    <td>T7-mutant5</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207029</th>
 +
    <td></td>
 +
    <td>T7-mutant6</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th>BBa_K2207030</th>
 +
    <td></td>
 +
    <td>T7-mutant7</td>
 +
    <td>Yuxing Chen</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
</tr>
 +
 +
  </table>
 
</div>
 
</div>
  
 +
  <h2 id="favouriteParts" class="H2Head">Favorite Parts!</h2>
 +
  <p class="PP">Our new Basic Parts in Trichoderma spp.</p>
 +
  <p class="PP">BBa_K2207003 ~ BBa_K2207008</p>
 +
  <p class="PP">We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker higB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate  these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.</p>
  
−
<div class="column half_size">
+
  <h2 id="BestCompositeParts" class="H2Head">Best Composite Parts</h2>
 +
  <p class="PP">BBa_K2207013</p>
 +
  <p class="PP">We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.</p>
 +
  <!--配DAPG基因簇图-->
  
−
<h5>Inspiration</h5>
+
  <h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
  <p class="PP">BBa_K2207024 ~ BBa_K2207030
 +
  <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.
 +
      <!--配IP结果处理图-->
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
 
</div>
 
</div>
 +
       
  
−
<div class="column full_size">
 
−
<h5>Part Table </h5>
 
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
<!-- 右侧监听开始 -->
 +
<div class="col-md-3 disappear-on-top" role="complementary">
 +
  <nav  style="position: fixed; top: 100px ; left:50px;" class="bs-docs-sidebar hidden-print hidden-xs hidden-sm">
 +
    <ul class="nav bs-docs-sidenav">
  
−
<div class="highlight">
+
      <li><a href="#introduction">Introduction</a></li>
 +
        <li><a href="#partTable">Parts Table</a></li>
 +
      <li><a href="#favouriteParts">Favourite Parts</a></li>
 +
        <li><a href="#BestCompositeParts">Best Composite Parts</a></li>
 +
        <li><a href="#ImproveT7promoters">Improve T7 promoters</a></li>
  
  
−
</html>
+
    </ul>
−
<groupparts>iGEM17 ZJU-China</groupparts>
+
  
−
<html>
+
  <a class="back-to-top" href="#top">
 +
  <!--  <i class="fa fa-arrow-up nav_icon"></i> -->
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 +
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 +
       
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 +
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 +
<!-- footer开始 -->
 +
<div class="footer"  style="background-color: #000000">
 +
  <div class="copyright container text-center">
 +
    <p class="copyright">Copyright &copy; 2017 ZJU-IGEM | <a href="http://www.zju.edu.cn/">Zhejiang University</a> |</p>
 +
    <p class="copyright">Contact us:<a class="copyright" href="">WeChat</a></p>
 +
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Revision as of 06:52, 13 October 2017

Parts

Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.

We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.

Parts
Name Type Description Designer Length(bp) Submitted
BBa_K2207000 Device H3-RP27 expression system Qianjin Jiang 2644
BBa_K2207001 Hex promoter Junbo Yang
BBa_K2207002 Ech42 promoter Junbo Yang
BBa_K2207003 Trichoderma HR System I SOD-Tubulin dobule promoter Yihe Zhang ✔
BBa_K2207004 Trichoderma HR System II Ech42-H3 double promoter Yihe Zhang ✔
BBa_K2207005 Trichoderma HR System III L1-ADH1-RP27-L2 Yihe Zhang ✔
BBa_K2207006 Trichoderma HR System IV L3-ADH1-RP27-L4 Yihe Zhang ✔
BBa_K2207007 Trichoderma HR System V Homologous Binding SiteA Yihe Zhang ✔
BBa_K2207008 Trichoderma HR System VI Homologous Binding SiteB Yihe Zhang ✔
BBa_K2207009 Coding Sequences PhlF transcription factor Qianjin Jiang 627 ✔
BBa_K2207010 Phl Operator Yuxing Chen 823
BBa_K2207011 Phl Operator(BBa_K2207010) mRFP Yuxing Chen ✔
BBa_K2207012 Composite Parts DAPG sensor(PhlOF) Qianjin Jiang 1450
BBa_K2207013 2,4-DAPG PhlABCD Cluster Junmin Qian ✔
BBa_K2207014 PhlAC enzyme Junmin Qian
BBa_K2207015 PhlBD enzyme Junmin Qian
BBa_K2207016 PhlAB enzyme Junmin Qian
BBa_K2207017 PhlCD enzyme Junmin Qian
BBa_K2207018 GPD-CYC1 expression system Shisheng Li
BBa_K2207019 TRPV1-Ferritin Cheng Shen
BBa_K2207020 Ferritin Cheng Shen
BBa_K2207021 Calcineurin-dependent response element (CDRE) Shisheng Li ✔
BBa_K2207022 CDRE(BBa_K2207021) mrfp Shisheng Li ✔
BBa_K2207023 Mature serine protein from Paecilomyces lilacinus Zifan Xie ✔
BBa_K2207024 T7-mutant1 Yuxing Chen ✔
BBa_K2207025 T7-mutant2 Yuxing Chen ✔
BBa_K2207026 T7-mutant3 Yuxing Chen ✔
BBa_K2207027 T7-mutant4 Yuxing Chen ✔
BBa_K2207028 T7-mutant5 Yuxing Chen ✔
BBa_K2207029 T7-mutant6 Yuxing Chen ✔
BBa_K2207030 T7-mutant7 Yuxing Chen ✔

Favorite Parts!

Our new Basic Parts in Trichoderma spp.

BBa_K2207003 ~ BBa_K2207008

We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker higB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.

Best Composite Parts

BBa_K2207013

We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.

Improve T7 promoters

BBa_K2207024 ~ BBa_K2207030

T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.

Copyright © 2017 ZJU-IGEM | Zhejiang University |

Contact us:WeChat