Difference between revisions of "Team:MSU-Michigan/Current"
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | <h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" width="366.5" height="300"> | + | <figure><img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" width="366.5" height="300"></figure> |
<p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p> | <p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p> | ||
<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | <h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" width="366.5" height="300"> | + | <figure><img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" width="366.5" height="300"></figure> |
<p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p> | <p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p> | ||
<h3>Multiple Strains</h3> | <h3>Multiple Strains</h3> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/d/d7/T--MSU-Michigan--current170714.png" width="366.5" height="300"> | + | <figure><img src="https://static.igem.org/mediawiki/2017/d/d7/T--MSU-Michigan--current170714.png" width="366.5" height="300"> |
| + | </figure> | ||
<p>Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.</p> | <p>Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.</p> | ||
<h3>Anaerobic Current</h3> | <h3>Anaerobic Current</h3> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/2/22/T--MSU-Michigan--currentanaerobic20170911.png" width="366.5" height="300"> | + | <figure><img src="https://static.igem.org/mediawiki/2017/2/22/T--MSU-Michigan--currentanaerobic20170911.png" width="366.5" height="300"></figure> |
<p>Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.</p> | <p>Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.</p> | ||
Revision as of 21:42, 14 October 2017
Contents
Current from Induction
deltamtrB_GFPmtrB Induced V Uninduced
<figure><img src="
" width="366.5" height="300"></figure>
Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.
deltamtrB_GFPmtrB Induced V Uninduced
<figure><img src="
" width="366.5" height="300"></figure>
The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.
Multiple Strains
<figure><img src="
" width="366.5" height="300">
</figure>
Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.
Anaerobic Current
<figure><img src="
" width="366.5" height="300"></figure>
Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.
