Difference between revisions of "Team:XMU-China/Safety"

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<span class="subtitle" id="subtitleone">Safe&nbsp;Project&nbsp;Design</span>
 
<span class="subtitle" id="subtitleone">Safe&nbsp;Project&nbsp;Design</span>
 
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<p>Firstly, E.coli BL21 and E.coli DH5α were the only chassis when we performed the molecular cloning and expression. Our bacteria will be engineered to detect metal ions, e.g., cobalt and mercury. We propose to construct an intermediate to normalize the response level of various ions. An induced expression system will be used to amplify the signal response to those ions. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. Besides, the main part of our product is reusable. </p>
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<p>Firstly, E.coli BL21 and E.coli DH5α were the only chassis when we performed the molecular cloning and expression. Our bacteria will be engineered to detect metal ions, e.g., cobalt and mercury. We propose to construct an intermediate to normalize the response level of various ions. An induced expression system will be used to amplify the signal response to those ions. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. Besides, the main part of our product is reusable. </p><span class="blank"></span>
 
<span class="safetyimg"><img class="safetyimgone" src="https://static.igem.org/mediawiki/2017/8/83/T--XMU-China--Safety-picture1.jpeg"></span>
 
<span class="safetyimg"><img class="safetyimgone" src="https://static.igem.org/mediawiki/2017/8/83/T--XMU-China--Safety-picture1.jpeg"></span>
 
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<span class="subtitle"id="subtitletwo">Safe&nbsp;Lab&nbsp;Work</span>
 
<span class="subtitle"id="subtitletwo">Safe&nbsp;Lab&nbsp;Work</span>
 
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<p>The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment , first aid kit and so on. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. Turn off the water and electricity, close the door, window and door when we leave the lab.</p>
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<p>The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment , first aid kit and so on. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. Turn off the water and electricity, close the door, window and door when we leave the lab.</p><span class="blank"></span>
 
<span class="safetyimg"><img class="safetyimgone" src="https://static.igem.org/mediawiki/2017/5/57/T--XMU-China--Safety-picture2.jpeg"></span>
 
<span class="safetyimg"><img class="safetyimgone" src="https://static.igem.org/mediawiki/2017/5/57/T--XMU-China--Safety-picture2.jpeg"></span>
 
<span class="blank"></span>
 
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Revision as of 06:56, 15 October 2017

2017.igem.org/Team:XMU-China/Safety

Safety
Safe Project Design

Firstly, E.coli BL21 and E.coli DH5α were the only chassis when we performed the molecular cloning and expression. Our bacteria will be engineered to detect metal ions, e.g., cobalt and mercury. We propose to construct an intermediate to normalize the response level of various ions. An induced expression system will be used to amplify the signal response to those ions. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. Besides, the main part of our product is reusable.

Safe Lab Work

The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment , first aid kit and so on. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. Turn off the water and electricity, close the door, window and door when we leave the lab.

Safe Shipment

We sent our BioBricks through the standard shipping process required by iGEM.