Difference between revisions of "Team:Kyoto/Experiments"
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<h4><a name="#Western">Basic protcol of Western blotting</a></h4> | <h4><a name="#Western">Basic protcol of Western blotting</a></h4> | ||
<h4><a name="#western2">Western blotting(Membrane fraction)</a></h4> | <h4><a name="#western2">Western blotting(Membrane fraction)</a></h4> | ||
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Revision as of 12:29, 15 October 2017
Material&Methods
- Parts
- Primer list
- Materials
- 3-1 Kit
- 3-2 Restricton Enzyme
- 3-3 Polymerase
- 3-4 DNA ligase
- 3-5 Marker
- 3-6 Organism
- 3-7 Antibiotics
- 3-8 Equipment
- 3-9 Backborns
- 3-10 Buffer
- 3-11 SDSPAGE/Western blotting
- Methods
- 4-1 Miniprep
- 4-2 Gel Extraction
- 4-3 Restrict Enzyme Digestion
- 4-4 Ligation
- 4-5 Transformation
- 4-6 PCR
- 4-7 Sequencing
- 4-8 RT-PCR
- 4-10 Western blotting(Membrane fraction)
Parts
Primer list
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Materials
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