Difference between revisions of "Team:BostonU/Contribution"

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  <p class="wide-heading-type mainwrap align-center">CONTRIBUTION TO THE INTERLAB STUDY</p>
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</div></div>
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  <p class="body-type mainwrap indented">Fluorescence measurements are difficult to standardize across labs because different methods and units are used to measure fluorescence. In an effort to standardize fluorescence measurements from Green Fluorescent Protein (GFP), one of the most commonly used marker proteins, iGEM has implemented the InterLab Study in order to provide protocols for users to generate absolute units for measuring GFP. This is the fourth year iGEM has done the InterLab Study. </p>
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  <p class="body-type mainwrap indented">&nbsp;</p>
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  <p class="body-type mainwrap indented">This year, iGEM is addressing this question: How close can the numbers be when fluorescence is measured all around the world? Parts from the iGEM InterLab Kit were transformed into competent E. coli DH5-alpha cells. Eight plasmids in total were transformed, including a Negative Control, a Positive Control, and six test devices.Their iGEM IDs are listed below. All measurements were made in a SpectraMax plate reader from a Costar black clear bottom 96-well plate. </p>
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  <ul class="link-slideup">
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  <p class="body-type mainwrap ">Positive Control (<a>BBa_I20270</a>)</p>
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  <p class="body-type mainwrap">Negative Control (<a>BBa_R0040</a>)</p>
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  <p class="body-type mainwrap">Test Device 1 (<a>BBa_J364000</a>)</p>
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  <p class="body-type mainwrap">Test Device 2 (<a>BBa_J364001</a>)</p>
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  <p class="body-type mainwrap">Test Device 3 (<a>BBa_J364002</a>)</p>
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  <p class="body-type mainwrap">Test Device 4 (<a>BBa_J364003</a>)</p>
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  <p class="body-type mainwrap">Test Device 5 (<a>BBa_J364004</a>)</p>
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  <p class="body-type mainwrap">Test Device 6 (<a>BBa_J364005</a>) </p>
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  <p class="body-type mainwrap">&nbsp;</p>
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<h1>Contribution</h1>
 
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<h3>Bronze Medal Criterion #4</h3>
 
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
 
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
 
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Revision as of 15:44, 16 October 2017

CONTRIBUTION TO THE INTERLAB STUDY

Fluorescence measurements are difficult to standardize across labs because different methods and units are used to measure fluorescence. In an effort to standardize fluorescence measurements from Green Fluorescent Protein (GFP), one of the most commonly used marker proteins, iGEM has implemented the InterLab Study in order to provide protocols for users to generate absolute units for measuring GFP. This is the fourth year iGEM has done the InterLab Study.

 

This year, iGEM is addressing this question: How close can the numbers be when fluorescence is measured all around the world? Parts from the iGEM InterLab Kit were transformed into competent E. coli DH5-alpha cells. Eight plasmids in total were transformed, including a Negative Control, a Positive Control, and six test devices.Their iGEM IDs are listed below. All measurements were made in a SpectraMax plate reader from a Costar black clear bottom 96-well plate.