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Revision as of 05:21, 17 October 2017
Model
Development of A Novel Blood-MicroRNA Handy Detection System with CRISPR
Abstract
This model is created to evaluate the effectiveness of initial design, and offers guidelines on how the system can (or must) be improved. (You can go to PROJECT. page to see more
Introduction
We create mathematical models of two aspects of our project, a RCA model and a signal detection model.
Assumption and Justification
About model
1. MiRNA is not degraded throughout the reaction process.
2. The two fusion proteins of dCas9 and split-HRP fragments have the same ability to combine with the stem-loop structure, and only when two different proteins next to each other, can they have the ability to catalyze substrate and produce signal.
3. The number of stem-loop structures in each RCA product is equal under a certain reaction time.
4. The enzymatic activity remains unchanged with time under the premise of excessive amount of enzymes or a short-time reaction.
About the data
1. The data we obtain from wet-lab experiment are reliable.
2. All the results are trustworthy in the process of statistical processing and data calculation.
Model
Notations
Figure 1. Schematic diagram
