Difference between revisions of "Team:Munich/Gold Integrated/KeithPardee"

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<b>We read your paper from last year titled “Rapid, Low-Cost Detection of Zika Virus Using
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<b class="interviewQuestion">We read your paper from last year titled “Rapid, Low-Cost Detection of Zika Virus Using
 
Programmable Biomolecular Components” and we wanted to know, what led you to
 
Programmable Biomolecular Components” and we wanted to know, what led you to
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use β-galactosidase as a colored readout?</b> <br>
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use β-galactosidase as a colored readout?</b> <br><br>
 
Dr. Pardee: At that time, we hadn’t considered using C2C2 (Cas13a). So, one of the reasons was
 
Dr. Pardee: At that time, we hadn’t considered using C2C2 (Cas13a). So, one of the reasons was
 
because of the enzyme activity. Single molecule of reporter could serve amplification, rather than
 
because of the enzyme activity. Single molecule of reporter could serve amplification, rather than

Revision as of 17:58, 17 October 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Interview with Dr. Keith Pardee

We read your paper from last year titled “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components” and we wanted to know, what led you to use β-galactosidase as a colored readout?

Dr. Pardee: At that time, we hadn’t considered using C2C2 (Cas13a). So, one of the reasons was because of the enzyme activity. Single molecule of reporter could serve amplification, rather than just fluorescence. The other (reason) was just for practical use. No need (to use) UV lights, electronics, camera, and could be done in a simple piece of paper. Tried 10 different enzyme options with other based reporters.
Diagram for Cas13a's function

iGEM BOKU Vienna

Michael and Julian from the iGEM BOKU Vienna came to do some experiments in our lab on 4th and 5th of October. Their iGEM project is called D.I.V.E.R.T. (Directed in vivo evolution via reverse transcription) and they are trying out new strategies for in vivo evolution which shows potential advantages over classical in vitro methods. For this they use yeast and E.coli to demonstrate their concept. They used the flow cytometer in our lab in Garching to better characterize their constructs from E.coli and S. cerevisiae. For the use one of our lab member explained them how to use the flow cytometer in the lab and also provided them the necessary help. It was a long day work for them but they got convincing results by the end. We also had a small gathering in the evening before they left together with the old igemers who came to meet us. We were very happy to have them in our lab and to get to know each other's team.