Difference between revisions of "Team:Kyoto/Experiments"
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<h1>Material&Methods</h1> | <h1>Material&Methods</h1> | ||
| − | < | + | <ul> |
| − | <li><a href="#Parts">Parts</a></li> | + | <li><a href="#Parts">1.Parts</a></li> |
| − | <li><a href="#Primer">Primer list</a></li> | + | <li><a href="#Primer">2.Primer list</a></li> |
| − | <li><a href="#Materials">Materials</a></li> | + | <li><a href="#Materials">3.Materials</a></li> |
<ul> | <ul> | ||
| − | <li><a href="#Kit">3-1 Kit</a></li> | + | <li><a href="#Kit">3-1. Kit</a></li> |
| − | <li><a href="#Enzyme">3-2 Restricton Enzyme</a></li> | + | <li><a href="#Enzyme">3-2. Restricton Enzyme</a></li> |
| − | <li><a href="#Polymerase">3-3 Polymerase</a></li> | + | <li><a href="#Polymerase">3-3. Polymerase</a></li> |
| − | <li><a href="#Ligase">3-4 DNA ligase</a></li> | + | <li><a href="#Ligase">3-4. DNA ligase</a></li> |
| − | <li><a href="#Marker">3-5 Marker</a></li> | + | <li><a href="#Marker">3-5. Marker</a></li> |
| − | <li><a href="#Organism">3-6 Organism</a></li> | + | <li><a href="#Organism">3-6. Organism</a></li> |
| − | <li><a href="#Antibiotics">3-7 Antibiotics</a></li> | + | <li><a href="#Antibiotics">3-7. Antibiotics</a></li> |
| − | <li><a href="#Equipment">3-8 Equipment</a></li> | + | <li><a href="#Equipment">3-8. Equipment</a></li> |
| − | <li><a href="#backbones">3-9 Backborns</a></li> | + | <li><a href="#backbones">3-9. Backborns</a></li> |
| − | <li><a href="#Buffer">3-10 Buffer</a></li> | + | <li><a href="#Buffer">3-10. Buffer</a></li> |
| − | <li><a href="#SDSPAGE">3-11 SDSPAGE/Western blotting</a></li> | + | <li><a href="#SDSPAGE">3-11. SDSPAGE/Western blotting</a></li> |
</ul> | </ul> | ||
| − | <li><a href="#Methods">Methods</a></li> | + | <li><a href="#Methods">4.Methods</a></li> |
<ul> | <ul> | ||
| − | <li><a href="#Miniprep">4-1 Miniprep</a></li> | + | <li><a href="#Miniprep">4-1. Miniprep</a></li> |
| − | <li><a href="#Gel">4-2 Gel Extraction</a></li> | + | <li><a href="#Gel">4-2. Gel Extraction</a></li> |
| − | <li><a href="#Digestion">4-3 Restrict Enzyme Digestion</a></li> | + | <li><a href="#Digestion">4-3. Restrict Enzyme Digestion</a></li> |
| − | <li><a href="#Ligation">4-4 Ligation</a></li> | + | <li><a href="#Ligation">4-4. Ligation</a></li> |
| − | <li><a href="#Transformation">4-5 Transformation</a></li> | + | <li><a href="#Transformation">4-5. Transformation</a></li> |
| − | <li><a href="#PCR">4-6 PCR</a></li> | + | <li><a href="#PCR">4-6. PCR</a></li> |
| − | <li><a href="#Sequencing">4-7 Sequencing</a></li> | + | <li><a href="#Sequencing">4-7. Sequencing</a></li> |
| − | <li><a href="#RTPCR">4-8 RT-PCR</a></li> | + | <li><a href="#RTPCR">4-8. RT-PCR</a></li> |
| − | <li><a href="#Western>4-9 Basic protocol of Western blotting</a></li> | + | <li><a href="#Western>4-9. Basic protocol of Western blotting</a></li> |
| − | <li><a href="#Western2">4-10 Western blotting(Membrane fraction)</a></li> | + | <li><a href="#Western2">4-10. Western blotting(Membrane fraction)</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <h3><a name="#Parts">Parts</a></h3> | + | <h3><a name="#Parts">1.Parts</a></h3> |
| − | <h3><a name="#Primer">Primer list</a></h3> | + | <h3><a name="#Primer">2.Primer list</a></h3> |
<table align="center"> | <table align="center"> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
| − | <h3><a name="#Materials">Materials</a></h3> | + | <h3><a name="#Materials">3.Materials</a></h3> |
<table align="center"> | <table align="center"> | ||
| − | <h5><a name="#Kit">Kit</a></h5> | + | <h5><a name="#Kit">3-1.Kit</a></h5> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Enzyme">Restriction Enzyme</a></h4> | + | <h4><a name="#Enzyme">3-2.Restriction Enzyme</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Polymerase"></a></h4> | + | <h4><a name="#Polymerase">3-3.Polymerase</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Ligase">DNA Ligase</a></h4> | + | <h4><a name="#Ligase">3-4.DNA Ligase</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Marker">Marker</a></h4> | + | <h4><a name="#Marker">3-5.Marker</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Organism"></a></h4> | + | <h4><a name="#Organism">3-6.Organism</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Antibiotics">Antibiotics</a></h4> | + | <h4><a name="#Antibiotics">3-7.Antibiotics</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Equipment">Equipment</a></h4> | + | <h4><a name="#Equipment">3-8.Equipment</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Backbones">Backbones</a></h4> | + | <h4><a name="#Backbones">3-9.Backbones</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#Buffer">Buffer</a></h4> | + | <h4><a name="#Buffer">3-10.Buffer</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
<table align="center"> | <table align="center"> | ||
| − | <h4><a name="#SDSPAGE">SDSPAGE/Western blotting</a></h4> | + | <h4><a name="#SDSPAGE">3-11.SDSPAGE/Western blotting</a></h4> |
<thead> | <thead> | ||
<tr> | <tr> | ||
| Line 243: | Line 248: | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| − | <h3><a name="#Methods">Methods</a></h3> | + | <h3><a name="#Methods">4.Methods</a></h3> |
| − | <h4><a name="#Miniprep">Miniprep</a></h4> | + | <h4><a name="#Miniprep">4-1.Miniprep</a></h4> |
| − | <h4><a name="#Gel">Gel Extraction</a></h4> | + | <h4><a name="#Gel">4-2.Gel Extraction</a></h4> |
| − | <h4><a name="#Digestion">Restriction Enzyme Digestion</a></h4> | + | <h4><a name="#Digestion">4-3.Restriction Enzyme Digestion</a></h4> |
| − | <h4><a name="#Ligation">Ligation</a></h4> | + | <h4><a name="#Ligation">4-4.Ligation</a></h4> |
| − | <h4><a name="#Transformation">Transformation</a></h4> | + | <h4><a name="#Transformation">4-5.Transformation</a></h4> |
| − | <h4><a name="#PCR">PCR</a></h4> | + | <h4><a name="#PCR">4-6.PCR</a></h4> |
| − | <h4><a name="#Sequencing">Sequenicing</a></h4> | + | <h4><a name="#Sequencing">4-7.Sequenicing</a></h4> |
| − | <h4><a name="#RTPCR">RT-PCR</a></h4> | + | <h4><a name="#RTPCR">4-8.RT-PCR</a></h4> |
| − | <h4><a name="#Western">Basic protcol of Western blotting</a></h4> | + | <h4><a name="#Western">4-9.Basic protcol of Western blotting</a></h4> |
| − | <h4><a name="#western2">Western blotting(Membrane fraction)</a></h4> | + | <h4><a name="#western2">4-10.Western blotting(Membrane fraction)</a></h4> |
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 13:23, 18 October 2017
Material&Methods
- 1.Parts
- 2.Primer list
- 3.Materials
- 3-1. Kit
- 3-2. Restricton Enzyme
- 3-3. Polymerase
- 3-4. DNA ligase
- 3-5. Marker
- 3-6. Organism
- 3-7. Antibiotics
- 3-8. Equipment
- 3-9. Backborns
- 3-10. Buffer
- 3-11. SDSPAGE/Western blotting
- 4.Methods
- 4-1. Miniprep
- 4-2. Gel Extraction
- 4-3. Restrict Enzyme Digestion
- 4-4. Ligation
- 4-5. Transformation
- 4-6. PCR
- 4-7. Sequencing
- 4-8. RT-PCR
- 4-10. Western blotting(Membrane fraction)
1.Parts
2.Primer list
| Primer name | Sequence | Length | Tm | GC% | Designer | Manufacturer |
|---|
3.Materials
| Name | Supplier |
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