Difference between revisions of "Team:NPU-China"

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                     <p>All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.<br>
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Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.</p>
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                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam viverra euismod odio, gravida pellentesque
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                     <p>All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.<br>
                        urna varius vitae.</p>
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Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.</p>
 
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Revision as of 15:16, 18 October 2017

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution, high energy consumption and it is unsustainable.
This year, we aim to use a green and environmentally friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.


Core Part

We use CEAS2 enzyme as the core part, but acrylic acid is a byproduct of CEAS2 enzyme, the wild type 's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic effect of CEAS2 enzyme.
We designed Ceas2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for better-worked Ceas2 mutants by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

Project Three

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam viverra euismod odio, gravida pellentesque urna varius vitae.

Project Four

All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.
Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.


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