This year, teams participating in the Interlab Study had to transform eight DNA test devices (see Table 1 below) provided in the distribution kit into E. coli DH5α cells and measure GFP fluorescence of the cells at four different time points. The purpose of the Interlab Study was to assess the reproducibility of fluorescence measurements across different labs and identify possible sources of variation in results obtained when following a common protocol. To determine possible sources of variation, the six test devices include all of the combinations of 3 related constitutive promoters with either the RBS B0034 or the bicistronic design element J364100.

Standard Curves Generation

The Interlab Study protocol required two standard curves to be generated: Ludox OD600 reference point and fluorescein fluorescence standard curve. To generate the OD600 curve, we added each LUDOX and water to 4 wells of the 96 well plate and measured OD600 with a plate reader. The results are shown in table 2.

To produce a fluorescein fluorescence standard curve, we made 4 sets of fluorescein serial dilutions in a 96 well plate by preparing 1x fluorescein solution in PBS and adding the appropriate amounts of fluorescein and PBS into 48 plate wells (see our notebook for more details). The curve generated is shown in figure 1 and figure 2.